Facebook Instagram Twitter RSS Feed PodBean Back to top on side

Long-Term BRCA1 Down-Regulation by Small Hairpin RNAs Targeting the 3’ Untranslated Region

In: NEOPLASMA, vol. 55, no. 2
E. Vondruskova - R. Malik - J. Sevcik - P. Kleiblova - Z. Kleibl
Detaily:
Rok, strany: 2008, 130 - 137
O článku:
Mutations in the BRCA1 gene are responsible for the majority of hereditary breast/ovarian cancers. The functional significance of many mutations/splicing variants identified during the screening of high-risk individuals is difficult to predict due to the lack of in vitro functional tests correlating sequence variants with a risk of cancer development. RNA interference is a promising tool in analyzing functional properties of BRCA1 mutations. Here we designed and functionally analyzed shRNAs directed to 3’-UTR of BRCA1 mRNA that may be used to knock-down expression of endogenous BRCA1. Using retroviral infection, we achieved long-term down-regulation of BRCA1 in a cell-type specific manner. We propose that 3’-UTR-directed shRNAs, coupled with up-regulation of exogenous mutated BRCA1 variants, may constitute a versatile system for the functional analysis of BRCA1 gene alterations. Key words: BRCA1, Retroviral infection, RNAi, shRNA, UTR
Ako citovať:
ISO 690:
Vondruskova, E., Malik, R., Sevcik, J., Kleiblova, P., Kleibl, Z. 2008. Long-Term BRCA1 Down-Regulation by Small Hairpin RNAs Targeting the 3’ Untranslated Region. In NEOPLASMA, vol. 55, no.2, pp. 130-137. 0028-2685.

APA:
Vondruskova, E., Malik, R., Sevcik, J., Kleiblova, P., Kleibl, Z. (2008). Long-Term BRCA1 Down-Regulation by Small Hairpin RNAs Targeting the 3’ Untranslated Region. NEOPLASMA, 55(2), 130-137. 0028-2685.