In: NEOPLASMA, vol. 55, no. 1
M. Svachova - M. Tichý
Detaily:
Rok, strany: 2008, 36 - 41
O článku:
To confirm a diagnosis of malignant lymphomas it is imperative to distinguish between reactive and neoplastic proliferation.
The PCR (polymerase chain reaction) is a method that can be used for detection of clonal rearrangements of the
immunoglobulin heavy chain (IgH) and T-cell receptor (TCR) genes. This study summarizes the outcomes of PCR analysis
of IgH and TCR gene rearrangements in 91 bioptic cases of lymphoproliferative disorders. In the class of B lymphomas we
detected clonal IgH rearrangement in nearly 83% of cases and in class of T lymphomas in 81% of cases. We can affirm that
PCR analysis of B and T cell clonality on DNA extracted from the whole section of formalin-fixed, paraffin-embedded
tissue is very suitable for routinely elaborate this. Its influence on the diagnostics of morphological unclear cases in particular,
is crucial and is useful in establishing a diagnosis of lymphoid neoplasias in specimens in which histological and
immunophenotypic studies are inconclusive.
Key words: malignant lymphoma, clonality gene rearrangement, polymerase chain reaction
Ako citovať:
ISO 690:
Svachova, M., Tichý, M. 2008. PCR analysis of immunoglobulin heavy chain and TCR gene
rearrangements in diagnosis of lymphoproliferative disorders
on formalin-fixed, paraffin-embedded tissues. In NEOPLASMA, vol. 55, no.1, pp. 36-41. 0028-2685.
APA:
Svachova, M., Tichý, M. (2008). PCR analysis of immunoglobulin heavy chain and TCR gene
rearrangements in diagnosis of lymphoproliferative disorders
on formalin-fixed, paraffin-embedded tissues. NEOPLASMA, 55(1), 36-41. 0028-2685.