In: NEOPLASMA, vol. 54, no. 5
S. Sufliarska - G. Minarik - J. Horáková - I. Bodova - E. Bojtarova - B. Czako - M. Mistrík - L. Drgona - M. J. - M. Krivosikova - L. Kovacs
Detaily:
Rok, strany: 2007, 424 - 430
O článku:
We describe the implementation, optimization, sensitivity determination and first clinical results of polymerase chain
reaction (PCR) amplification of polymorphic short tandem repeat (STR) markers and Amelogenin locus coupled with
fluorescent detection and capillary electrophoresis in chimerism monitoring of patients transplanted at three different transplant
centers using a commercially available multiplex microsatellite assay. The chimerism analysis was performed with genomic
DNA extracted from unselected peripheral blood leukocytes of one hundred pediatric and adult patients, who underwent
allogeneic stem cell transplantation (SCT) from human leukocyte antigen (HLA) matched or one antigen mismatched
related or unrelated donors for malignant (70 patients) and non-malignant (30 patients) diseases. Tested were 79 donor
recipient pairs for 15 STR systems and identified an informative marker in all but one of them (98,7%), using 6 selected
systems out of these fifteen, that appeared highly informative in our patients’ population. In 21 sex-mismatched donor
recipient pairs we used the Amelogenin locus to distinguish the X and Y chromosome. In sixty-three out of these 100
patients chimerism was regularly analyzed from blood samples taken at various time points after SCT with the median
follow up of 17 months. Complete chimerism (CC), maintained over the whole follow-up period, was detected in 24 (38,
1%), stable and decreasing mixed chimerism (MC) in 28 (44, 4%) and increasing MC in 11 patients (17, 5%). Patients with
CC, stable and decreasing MC showed a significantly better (p 0,005) overall survival rate (0, 81), compared to those with
increasing MC (0, 24). These results demonstrate that STR-based chimerism monitoring with sensitivity above 1% and high
informativity (98, 7% of donor recipient pairs) is necessary in establishing the origin of engrafted cells after an allogeneic
SCT, in detecting graft rejection and that it may contribute in identifying patients with imminent leukemia relapse.
Key words: allogeneic stem cell transplantation, chimerism monitoring, polymerase chain reaction, short tandem repeat
markers, complete chimerism, decreasing mixed chimerism, increasing mixed chimerism
Ako citovať:
ISO 690:
Sufliarska, S., Minarik, G., Horáková, J., Bodova, I., Bojtarova, E., Czako, B., Mistrík, M., Drgona, L., J., M., Krivosikova, M., Kovacs, L. 2007. Establishing the method of chimerism monitoring after allogeneic stem
cell transplantation using multiplex polymerase chain reaction
amplification of short tandem repeat markers and Amelogenin. In NEOPLASMA, vol. 54, no.5, pp. 424-430. 0028-2685.
APA:
Sufliarska, S., Minarik, G., Horáková, J., Bodova, I., Bojtarova, E., Czako, B., Mistrík, M., Drgona, L., J., M., Krivosikova, M., Kovacs, L. (2007). Establishing the method of chimerism monitoring after allogeneic stem
cell transplantation using multiplex polymerase chain reaction
amplification of short tandem repeat markers and Amelogenin. NEOPLASMA, 54(5), 424-430. 0028-2685.