In: NEOPLASMA, vol. 54, no. 4
P. Plevova - J. Bouchal - M. Fiuraskova - M. Papežová - A. Křepelová - R. Čuřík - L. Foretová - M. J. - T. Posolda - Z. Kolar
Detaily:
Rok, strany: 2007, 269 - 277
O článku:
The PML (promyelocytic leukemia) protein is concentrated in the PML nuclear bodies. In human cell lines and tumors
maintaining their telomeres by alternative lengthening (ALT), the PML protein is colocalized with TRF2 and several other
proteins in the so called ALT-associated PML bodies. The aim of this study was to determine if there is any difference in
PML protein expression between tumors with stable microsatellites (MSS) and those with high-frequency microsatellite
instability (MSI-H), if PML protein expression might be a prognostic factor and if MSI-H tumors more frequently use
alternative lengthening of telomeres measured by the presence of ALT-associated PML bodies. Eighty colorectal cancer
samples (32 MSI-H and 48 MSS) and 8 human tumor cell lines (Saos-2, U2OS, DU145, LNCaP, U87, HeLa, MCF7 and
T98G) were included into the study. Double-colour immunofluorescence staining was used. Downregulation of PML protein
expression was found in 7 of 32 (22%) MSI-H and 11 of 48 (23%) MSS tumors (p=0.520). There was no correlation
between PML expression and age, histological typing, localization of the tumor in colon, TNM classification, disease-free
and overall survival. The Saos-2 and U2OS (ALT using cell lines) and the MCF7 (active telomerase) cell line were characterized
by the presence of ALT-associated PML bodies; no such bodies were detected in the DU145, LNCaP, U87, HeLa and T98G
cell lines (active telomerase); accumulation of TRF2 was absent or much weaker in these cell lines compared to Saos-2 or
U2OS. Accumulation of the TRF2 protein was detected in 16 of 80 (20%) tumors and PML and TRF2 colocalization in 2
MSI-H tumors (6%). In conclusion, the PML protein was downregulated in approximately 20% of tumors; there was no
difference between MSS and MSI-H tumors. PML protein expression does not seem to be a prognostic factor.
Key words: PML; TRF2; alternative lengthening of telomeres; hMLH1 – gene; hMSH2 – gene; microsatellite instability
Ako citovať:
ISO 690:
Plevova, P., Bouchal, J., Fiuraskova, M., Papežová, M., Křepelová, A., Čuřík, R., Foretová, L., J., M., Posolda, T., Kolar, Z. 2007. PML and TRF2 protein expression in hereditary
and sporadic colon cancer. In NEOPLASMA, vol. 54, no.4, pp. 269-277. 0028-2685.
APA:
Plevova, P., Bouchal, J., Fiuraskova, M., Papežová, M., Křepelová, A., Čuřík, R., Foretová, L., J., M., Posolda, T., Kolar, Z. (2007). PML and TRF2 protein expression in hereditary
and sporadic colon cancer. NEOPLASMA, 54(4), 269-277. 0028-2685.