In: NEOPLASMA, vol. 53, no. 6
R. Voboril - J. Weberova-Voborilova
Detaily:
Rok, strany: 2006, 518 - 523
O článku:
Constitutive NF-kappaB activity has been found in many cancer cells of different origin. In our study we focused on constitutive
NF-kappaB activity and its impact on radiation-induced NF-kappaB activity, intrinsic radiosensitivity, and apoptosis. Using
colorectal cancer cell lines (Caco-2, SW480, SW620) we demonstrated that each cell line expresses different level of constitutive
NF-kappaB activity. Moreover, irradiation caused secondary NF-kappaB activation which differed in each cell line. The cell
lines tested displayed also different intrinsic radiosensitivities as was determined by clonogenic assay, and different spontaneous
and radiation-induced apoptosis determined by activity of caspase 3. Complex analysis of our results revealed that
there was a strong correlation between constitutive NF-kappaB activity and radiation-induced NF-kappaB activity (r=0.835), the
level of constitutive NF-kappaB activity predicted the level of secondary, radiation-induced NF-kappaB activity. Furthermore,
SW620 cells with the highest level of constitutive NF-kappaB activity displayed the lowest radiosensitivity and the lowest level
of spontaneous apoptosis; Caco-2 cells with almost undetectable level of constitutive NF-kappaB activity displayed the highest
radiosensitivity and even highest level of spontaneous apoptosis. SW480 cells showed intermediate level of constitutive
NF-kappaB activity, intermediate radiosensitivity and intermediate level of spontaneous apoptosis. Our data suggest that the
level of constitutive NF-kappaB activity may predict radiosensitivity of colorectal cancer cells. Such prediction may allow the
individualization of patient treatment by radiotherapy.
Ako citovať:
ISO 690:
Voboril, R., Weberova-Voborilova, J. 2006. Constitutive NF-kappaB activity in colorectal cancer cells: impact on radiation-induced NF-kappaB activity, radiosensitivity, and apoptosis. In NEOPLASMA, vol. 53, no.6, pp. 518-523. 0028-2685.
APA:
Voboril, R., Weberova-Voborilova, J. (2006). Constitutive NF-kappaB activity in colorectal cancer cells: impact on radiation-induced NF-kappaB activity, radiosensitivity, and apoptosis. NEOPLASMA, 53(6), 518-523. 0028-2685.