In: NEOPLASMA, vol. 53, no. 5
J. Ugorčáková - T. Hlavatý - P. Babál - P. Janega - G. Bukovská - N. Halgašová - A. Hlavatá - J. Timko - I. Duriš
Detaily:
Rok, strany: 2006, 363 - 371
O článku:
The aim of our work was to develop a fast, reliable and sensitive
PCR method to detect K-ras mutations in various clinical
samples. There is a need for an unimpeachable method for early
diagnosis and/or screening of pancreatic cancer (PC).
We optimized and subsequently analyzed four methods based on
mutant-enriched PCR for the sensitivity, cost and time
expense. Using the selected optimal method we examined codon 12 K-
ras mutations in a study population of 59 patients
with upper GIT malignancies. Reliability of the genotyping was
confirmed by sequencing.
By using the best of our modified mutant-enriched PCR methods we
achieved sensitivity of 1:1x10(exp5). Further studies are
necessary to determine the optimal biological material sampling in
PC.
Ako citovať:
ISO 690:
Ugorčáková, J., Hlavatý, T., Babál, P., Janega, P., Bukovská, G., Halgašová, N., Hlavatá, A., Timko, J., Duriš, I. 2006. Assay design and optimization of mutant-enriched PCR based method
for detection of K-ras gene mutations in pancreatic carcinoma. In NEOPLASMA, vol. 53, no.5, pp. 363-371. 0028-2685.
APA:
Ugorčáková, J., Hlavatý, T., Babál, P., Janega, P., Bukovská, G., Halgašová, N., Hlavatá, A., Timko, J., Duriš, I. (2006). Assay design and optimization of mutant-enriched PCR based method
for detection of K-ras gene mutations in pancreatic carcinoma. NEOPLASMA, 53(5), 363-371. 0028-2685.