In: NEOPLASMA, vol. 53, no. 3
B. Štabuc - Ž. Mrevlje - J. Markovič - M. Štabuc-Šilih
Detaily:
Rok, strany: 2006, 259 - 262
O článku:
Cathepsins are papain-like lysosome cystein proteases involved in
tumor growth, invasiveness and spread, angiogenesis
and alteration in immune and inflammatory responses. We
investigated the differences in cathepsin L (CatL) concentrations
in primary cutaneous malignant melanoma stage I and normal skin
and correlated these values with well-established malignant
melanoma prognostic factors.
The study was performed on 36 patients (17 men and 19 women; mean
age 54 years; range 21-84 years) with histological
confirmed primary malignant melanomas less than 1.5 mm thick. The
CatL concentrations were measured in 36 pairs of triton
extracts of cytosols prepared from the tumor and adjacent normal
tissue samples (matched pairs). The CatL concentrations
were determined by commercially available enzyme-liked
immunosorbent (ELISA) assay from KRKA (Novo Mesto,
Slovenia).
Significantly higher concentrations of CatL were detected in
malignant melanomas than in normal surrounding skin (6.73
vs. 1.42 ng/mg total protein (mgp), p<0.001). Significant
correlations between malignant melanoma and normal skin
concentrations
for CatL were found. The malignant melanoma CatL concentrations
correlated significantly with normal skin
(r=0.38; p=0.021).
CatL concentrations were significantly lower (p<0.01) in the
malignant melanomas of Breslow thickness <=0.75 mm,
Clark invasion <II, without microscopic ulceration and without
vascular invasion (4.14, 4.73, 6.15, 5.29 ng/mgp, respectively)
than in the malignant melanomas of Breslow thickness >0.75 mm,
Clark invasion of >=II and <=III, with microscopic
ulceration and with vascular invasion (7.67, 7.41, 9.15, 10.35
ng/mgp, respectively).
Higher CatL concentrations in early primary malignant melanomas
indicate its possible involvement in the processes of
early metastatic spread and its association with poor prognosis.
Ako citovať:
ISO 690:
Štabuc, B., Mrevlje, Ž., Markovič, J., Štabuc-Šilih, M. 2006. Expression and prognostic significance of Cathepsin L in early cutaneous malignant melanoma. In NEOPLASMA, vol. 53, no.3, pp. 259-262. 0028-2685.
APA:
Štabuc, B., Mrevlje, Ž., Markovič, J., Štabuc-Šilih, M. (2006). Expression and prognostic significance of Cathepsin L in early cutaneous malignant melanoma. NEOPLASMA, 53(3), 259-262. 0028-2685.