In: NEOPLASMA, vol. 53, no. 1
J. Munoz - J.s. Castresana
Detaily:
Rok, strany: 2006, 15 - 18
O článku:
DMBT1 is one of the putative suppressor genes on 10q25-qter, which
frequently lacks expression in many different kind
of tumors, such as glioblastoma, and lung, esophageal and
colorectal cancer. However, little is known about the reasons for
this lack of expression in neoplasia. In a previous report, our
group demonstrated how MC-IXC, a neuroblastoma cell line
which lacked DMBT1 expression, restored it after a 5-Aza-2'-deoxycitidine treatment. So, we wondered whether DMBT1
aberrant promoter methylation could be responsible for DMBT1
silencing in several tumor cell lines, in spite of the fact that
there is no CpG island near the 5' end of the gene. We studied the
possibility that methylation in CCWGG sequences of the
DMBT1 promoter (where "W" means "A" or "T") is able to silence the
gene, as had previously been reported for TP53 in
leukemia. We digested genomic DNA by the methylation sensitive
restriction enzyme EcoR II (C|CWGG), and made two
PCRs to amplify the three CCWGG domains placed in the 1kb upstream
DMBT1 5' end. After the PCRs, we could not find
correlation between methylation in CCWGG domains and DMBT1 lack of
expression. Apositive regulator of DMBT1 might
be silenced by aberrant methylation.
Ako citovať:
ISO 690:
Munoz, J., Castresana, J. 2006. Silencing of DMBT1 in neuroblastoma cell lines is not due to methylation of CCWGG motifs on its promoter. In NEOPLASMA, vol. 53, no.1, pp. 15-18. 0028-2685.
APA:
Munoz, J., Castresana, J. (2006). Silencing of DMBT1 in neuroblastoma cell lines is not due to methylation of CCWGG motifs on its promoter. NEOPLASMA, 53(1), 15-18. 0028-2685.