In: NEOPLASMA, vol. 53, no. 1
Y.-L. Sun - G.-Y. Zhou - K.-N. Li - P. Gao - Q.-H. Zhang - J.-H. Zhen - Y.-H. Bai - X.-F. Zhang
Detaily:
Rok, strany: 2006, 1 - 8
O článku:
Glucosylceramide synthase (GCS), the enzyme that converts ceramide
to glucosylceramide, induce multidrug resistance
(MDR) in cancer cells. Recently, RNA interference (RNAi) is a
powerful strategy for gene therapy by introducing double-stranded RNA and leading to the sequence-specific destruction. We
have designed two different short hairpin RNAs
(shRNAs) targeting GCS and introduced them into adriamycin-
resistant human breast cancer cells (MCF-7/AdrR cells) to inhibit
GCS expression. The results demonstrated that the shRNAs targeting
GCS decreased GCS mRNA, abolished GCS protein
levels and restored the sensitivity of MCF-7/AdrR cells to several
antineoplastic drugs. This study revealed that this approach
can reverse MDR effectively and it may be applicable to cancer
patients as a specific means to restore the sensitivity
to chemotherapy.
Ako citovať:
ISO 690:
Sun, Y., Zhou, G., Li, K., Gao, P., Zhang, Q., Zhen, J., Bai, Y., Zhang, X. 2006. Suppression of glucosylceramide synthase by RNA interference reverses multidrug resistance in human breast cancer cells. In NEOPLASMA, vol. 53, no.1, pp. 1-8. 0028-2685.
APA:
Sun, Y., Zhou, G., Li, K., Gao, P., Zhang, Q., Zhen, J., Bai, Y., Zhang, X. (2006). Suppression of glucosylceramide synthase by RNA interference reverses multidrug resistance in human breast cancer cells. NEOPLASMA, 53(1), 1-8. 0028-2685.