In: NEOPLASMA, vol. 52, no. 6
O. Babušíková - T. Železníková - A. Mlčáková - J. Kusenda - L. Števulová
Detaily:
Rok, strany: 2005, 502 - 509
O článku:
Bone marrow hematogones (benign B-lymphocyte precursors) may cause
diagnostic problems due to their morphologic
and immunophenotypic similarities with neoplastic lymphoblasts.
Hematogone populations in presented study containing
358 bone marrow specimens of 251 individuals always exhibited a
continuous and complete maturation spectrum of antigen
expression typical for normal evolution of B-lineage precursors;
lacking aberrant or asynchronous antigen expression. In
contrast lymphoblasts of 19 bone marrows of precursors B-ALL
patients showed maturation arrest and exhibited several
immunophenotypic aberrancies. Hematogones were identified by 4-color flow cytometry using optimal antibody combinations
in many bone marrow samples. They were more commonly found in
higher numbers in children, and there was found
a general decline in hematogones with increasing age. Bone marrow
hematogones were separately assessed as hematogones
1 population of early stage and hematogones 2 of mid-stage
precursor B-cells, respectively. In some (about 30%) of
hematogones a third type hematogones could be assessed in bone
marrow samples. This small B-cell subpopulation was defined
by CD10-positivity, coexpressing more mature markers
CD19,CD20,CD22 and CD45bright. These cells obviously
blended with those of mature B-lymphocytes (CD10-negative) on
CD45/SSC, and could be better recognized on
CD10-gating. Quantitative immunophenotyping of this study
completed the percent antigen expression data in two main
hematogone subtypes and lymphocytes in 16 bone marrow specimens
and precursor B-ALL lymphoblasts in some samples.
Increased information on benign B-lymphocyte precursors,
especially that of existence of ´the 3rd type hematogones´
could provide a basis for better discrimination of B-leukemia
cells even in a very small amounts.
Ako citovať:
ISO 690:
Babušíková, O., Železníková, T., Mlčáková, A., Kusenda, J., Števulová, L. 2005. The knowledge on the ´3rd type hematogones´ could contribute to more precise detection of small numbers of precursor B-acute lymphoblastic leukemia. In NEOPLASMA, vol. 52, no.6, pp. 502-509. 0028-2685.
APA:
Babušíková, O., Železníková, T., Mlčáková, A., Kusenda, J., Števulová, L. (2005). The knowledge on the ´3rd type hematogones´ could contribute to more precise detection of small numbers of precursor B-acute lymphoblastic leukemia. NEOPLASMA, 52(6), 502-509. 0028-2685.