Regulation of HBV replication and gene expression by miR-501-3p via targeting ZEB2 in hepatocellular carcinoma

Rok, strany: 2020, 735 - 742

Hepatitis B virus (HBV) infection is a major public health issue with serious medical consequences. The roles of microRNAs (miRNAs) in HBV replication and expression have been generally recognized, and the abnormal expression of miR-501 has been reported in patients with HBV infection. However, the function and mechanism in HBV replication remain elusive. The expression patterns of miR-501-3p and ZEB2 in HBV-related hepatocellular carcinoma (HCC) tissues and HBV-expressing HCC cells were determined by qRT-PCR and western blot assays. HBV replication and expression were evaluated through detecting the copies of HBV DNA and the secretion of HBV surface antigens HBsAg and HBeAg by real-time PCR and ELISA analyses. Bioinformatics and dual-luciferase reporter analyses were employed to validate the functional interaction between miR-501-3p and ZEB2. miR-501-3p was significantly upregulated, while ZEB2 was downregulated in HBV-related HCC tissues and cells compared with relative controls without HBV infection. Knockdown of miR-501-3p hampered HBV replication and gene expression in HepG2.2.15 and HepAD38 cells. ZEB2 was identified as a functional target of miR-501-3p. The absence of ZEB2 abolished the inhibitory effects of anti-miR-501-3p on HBV replication and gene expression. Our data indicated that miR-501-3p participated in the regulation of HBV replication and gene expression partially via repressing ZEB2 in HepG2.2.15 and HepAD38 cells, providing a promising antiviral avenue for HBV infection.

ISO 690:

Zhong, Z., Xu, J., Li, Z., Zhou, W., Chen, X., Zhou, J., Li, Z. 2020. Regulation of HBV replication and gene expression by miR-501-3p via targeting ZEB2 in hepatocellular carcinoma. In NEOPLASMA, vol. 67, no.4, pp. 735-742. 0028-2685. DOI: https://doi.org/10.4149/neo_2020_190625N549

APA:

Zhong, Z., Xu, J., Li, Z., Zhou, W., Chen, X., Zhou, J., Li, Z. (2020). Regulation of HBV replication and gene expression by miR-501-3p via targeting ZEB2 in hepatocellular carcinoma. NEOPLASMA, 67(4), 735-742. 0028-2685. DOI: https://doi.org/10.4149/neo_2020_190625N549

Vydavateľ: AEPress, Ltd.