In: NEOPLASMA, vol. 52, no. 5
J. R. Chen - Y.j. Lee - T. Chen - K.l. Wang - C.w. Dang - S.c. Chang - H.f. Liu - Y. C. Yang
Detaily:
Rok, strany: 2005, 369 - 373
O článku:
Cervical cancer has been one of the most important gynecologic
cancer in Taiwan with incidence of 24/100,000 and mortality
of 8.7/100,000 annually. About 70-80% are squamous cell carcinoma;
the remainder are composed of various types of
adenocarcinoma, adenosquamous carcinoma and undifferentiated
carcinoma. The Major Histocompatibility Complex
(MHC) class I chain-related gene A (MICA) is expressed by
keratinocytes and epithelial cells and interacts with
gamma-delta T cells. Although MICA was not associated with
cervical cancer in the study of Northern Sweden, there are no
further studies about the association of MICA polymorphism and the
different histological types of cervical cancer.
We analyzed the MICA polymorphism in 110 cervical cancer cases (88
squamous cell carcinoma, 12 adenocarcinoma
and 10 adenosquamous carcinoma) and 82 randomly selected unrelated
controls from 1994 to 2000 in the Mackay Memorial
Hospital, Taipei, Taiwan. DNA was extracted part from leukocytes
of peripheral blood, part from tumor tissue and 5 polymorphic
microsatellite alleles (A4,A5,A5.1,A6,A9) of MICA were identified
by a polymerase chain reaction-based (PCR)
technique using ABI Prism 377-18 DNA sequencer (Applied
Biosystems, Foster City, CA, USA).
The phenotypes, alleles and genotypes of MICA gene were
calculated. There was no association with cervical cancer patients
and non-cervical cancer patients (p=0.337, 0.356 and 0.414). After
dividing the cervical cancer patients into 3 major
histological types (squamous cell carcinoma, adenocarcinoma and
adenosquamous carcinoma), the result was still the same
(p=0.598, 0.172 and 0.617) in our study.
We found no association between MICA gene polymorphism and
cervical cancer in Taiwan. Different histological types
of cervical cancer also have no significant correlation with MICA
gene polymorphism. It demonstrates that polymorphism
of MICA gene bears no relation to cervical cancer and the
different histological types of cervical cancer in Taiwan. We need
further studies for identifying the factors causing the
differentiation of cancer cells of the uterine cervix.
Ako citovať:
ISO 690:
Chen, J., Lee, Y., Chen, T., Wang, K., Dang, C., Chang, S., Liu, H., Yang, Y. 2005. MHC class I chain-related gene A (MICA) polymorphism and the
different
histological types of cervical cancer. In NEOPLASMA, vol. 52, no.5, pp. 369-373. 0028-2685.
APA:
Chen, J., Lee, Y., Chen, T., Wang, K., Dang, C., Chang, S., Liu, H., Yang, Y. (2005). MHC class I chain-related gene A (MICA) polymorphism and the
different
histological types of cervical cancer. NEOPLASMA, 52(5), 369-373. 0028-2685.