In: NEOPLASMA, vol. 52, no. 4
M. Wachulska - K. Kozlowska - M. Cichorek
Detaily:
Rok, strany: 2005, 280 - 286
O článku:
The study concerns DNA ploidy and proliferative activity of the
cells of two hamster transplantable melanoma lines - differing
in many biological features - in the light of possible changes in
their secretory activity. DNA ploidy was determined
from the index of propidium iodide (PI) stained DNA content (DI),
while the proliferative activity was defined as the percentage
of cells in S and G2/M cell cycle phases. The secretory activity
was described by determining total protein,
interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-alpha), oncostatin
M (OSM), interleukin 10 (IL-10) and nitric oxide (NO)
content in the supernatants after 1, 6, 24 and 48 hours in cell
culture.
Our results indicate that melanotic melanoma cells (Ma) have a
near-tetraploid DNAcontent and about 18% of proliferating
cells, while amelanotic melanoma cells (Ab) - have a near-triploid
DNA content and almost twice as many proliferating
cells. The Ab cells, in comparison with Ma cells, secreted in
vitro less total protein and most of the cytokines examined except
OSM, but a spontaneous alteration of transplantable melanoma was
accompanied by an increase of the quantity and dynamics
of NO secretion.
So, the cells of two melanoma lines have their own characteristic
pattern of secretory function. But, the aneuploidy which
accompanied the changes in cell differentiation of the studied
melanoma lines, although seemed to reflect their changes in
the proliferative activity, nevertheless did not reflect, in a
direct way, differences in the secretion of the substances studied.
Ako citovať:
ISO 690:
Wachulska, M., Kozlowska, K., Cichorek, M. 2005. The DNA ploidy and proliferative activity of transplantable
melanoma cells in regard to their secretory function. In NEOPLASMA, vol. 52, no.4, pp. 280-286. 0028-2685.
APA:
Wachulska, M., Kozlowska, K., Cichorek, M. (2005). The DNA ploidy and proliferative activity of transplantable
melanoma cells in regard to their secretory function. NEOPLASMA, 52(4), 280-286. 0028-2685.