Comparison of HLA-G and MMP transcription in human tumor cell lines

Rok, strany: 2018, 943 - 951

HLA-G antigens and matrix metalloproteinases (MMPs) expressed in various tumors are involved in tumor growth and metastasis. In this study, we investigated if correlation between HLA-G and MMP expression exists in different cell lines. We examined MMP transcription in two choriocarcinoma cell lines: JEG-3 (HLA-G positive) and JAR (HLA-G negative). We discovered that both cell lines express a similar panel of MMPs; except for MMP12. Transcript MMP12 was exclusively detected in HLA-G expressing JEG-3 cells but not in HLA-G deficient JAR cells. We observed HLA-G expression but no MMP12 transcription following 5-aza-2´-deoxycytidine (AZA) treatment of JAR cells. We then investigated HLA-G and MMP transcription in several human leukaemia cell lines. Leukaemia cells (lacking HLA-G expression) were converted to their HLA-G positive counterparts by AZA-treatment or by HLA-G transfection. It was found no correlation between HLA-G and MMP transcription in any examined leukaemia cell lines. The up-regulation of some MMPs and tissue inhibitors of matrix metalloproteinases (TIMPs) was observed following AZA-treatment.

ISO 690:

Polakova, K., Bandzuchova, E. 2018. Comparison of HLA-G and MMP transcription in human tumor cell lines. In NEOPLASMA, vol. 65, no.6, pp. 943-951. 0028-2685.

APA:

Polakova, K., Bandzuchova, E. (2018). Comparison of HLA-G and MMP transcription in human tumor cell lines. NEOPLASMA, 65(6), 943-951. 0028-2685.