In: NEOPLASMA, vol. 51, no. 5
K. Horváthová - L. Novotný - D. Tóthová - A Vachálková
Detaily:
Rok, strany: 2004, 395 - 399
O článku:
We investigated protective effects of four flavonoids against H2O2-
induced DNA damage in human myelogenous leukemia cells (K562)
using the comet assay. The structural difference of studied
flavonoids - quercetin, rutin, luteolin and apigenin - are
characterized by the number of hydroxyl groups on the B ring. The
presence of an o-dihydroxy structure on the B-ring confers a
higher degree of stability to the flavonoid phenoxyl radicals by
participating in electron delocalization and is, therefore, an
important determinant for antioxidative potential. The results
correlate with earlier published data obtained in murine leukemia
cell line L1210. Hydrogen peroxide induced in human K562 cells a
concentration-dependent increase of single cell DNA strand breaks.
The strongest inhibition against H2O2-induced DNA damage (44%,
42%) was found in a range of luteolin and quercetin concentrations
of 20-100 micromol/l. Protective effect of rutin (100 and 1000
micromol/l) was only marginal (8-10%). Apigenin had no protective
effect on DNA single strand breaks induced by H2O2. Luteolin and
quercetin are therefore effective in the protection of human
single cell DNA from oxidative attack.
Ako citovať:
ISO 690:
Horváthová, K., Novotný, L., Tóthová, D., Vachálková, A. 2004. Determination of free radical scavenging activity of quercetin,
rutin, luteolin and apigenin in H2O2-treated human ML cells K562. In NEOPLASMA, vol. 51, no.5, pp. 395-399. 0028-2685.
APA:
Horváthová, K., Novotný, L., Tóthová, D., Vachálková, A. (2004). Determination of free radical scavenging activity of quercetin,
rutin, luteolin and apigenin in H2O2-treated human ML cells K562. NEOPLASMA, 51(5), 395-399. 0028-2685.