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MicroRNA-300 targets hypoxia inducible factor-3 alpha to inhibit tumorigenesis of human non-small cell lung cancer

In: NEOPLASMA, vol. 64, no. 4
Y. Zhang - Y. Guo - C. Yang - S. Zhang - X. Zhu - L. Cao - W. Nie - H. Yu
Detaily:
Rok, strany: 2017, 554 - 562
O článku:
Non-small cell lung cancer (NSCLC) is one of the most deadly human cancers. MicroRNA-300 acts as both tumor promoter and suppressor in different types of cancer. Here, we try to identify the function of microRNA-300 in human NSCLC. We compared MicroRNA-300 levels between tumor tissues versus paired adjacent non-tumor lung tissues from NSCLC patients, and in NSCLC versus normal lung cell lines. Effects of microRNA-300 on cell proliferation, invasion and migration were examined in vitro, and on tumor growth in vivo using a xenograft mouse model. Potential mRNA targets of microRNA-300 were predicted and underlying mechanism was explored. MicroRNA-300 expression was lower in both NSCLC tissues and cell lines. Overexpression of microRNA-300 inhibited proliferation, invasion and migration of NSCLC cells in vitro, and tumor growth in vivo. MicroRNA-300 could directly bind to the 3’-UTR of hypoxia inducible factor-3 alpha (HIF3α) mRNA, and inhibit both its mRNA and protein expressions. Restoring HIF3α expression could rescue the inhibitory effects of microRNA-300 on tumorigenesis of NSCLC both in vitro and in vivo. MicroRNA-300 is a tumor suppressor microRNA in NSCLC by downregulating HIF3α expression. Both microRNA-300 and HIF3α may serve as potential therapeutic targets in NSCLC treatment. Keywords: Non-small cell lung cancer, microRNA-300, tumor, xenograft, hypoxia inducible factor-3 alpha
Ako citovať:
ISO 690:
Zhang, Y., Guo, Y., Yang, C., Zhang, S., Zhu, X., Cao, L., Nie, W., Yu, H. 2017. MicroRNA-300 targets hypoxia inducible factor-3 alpha to inhibit tumorigenesis of human non-small cell lung cancer. In NEOPLASMA, vol. 64, no.4, pp. 554-562. 0028-2685.

APA:
Zhang, Y., Guo, Y., Yang, C., Zhang, S., Zhu, X., Cao, L., Nie, W., Yu, H. (2017). MicroRNA-300 targets hypoxia inducible factor-3 alpha to inhibit tumorigenesis of human non-small cell lung cancer. NEOPLASMA, 64(4), 554-562. 0028-2685.