Dental pulp mesenchymal stem/stromal cells labeled with iron sucrose release exosomes and cells applied intra-nasally migrate to intracerebral glioblastoma

In: NEOPLASMA, vol. 63, no. 6

U. Altanerova - K. Benejova - V. Altanerová - S. Tyciakova - B. Rychlý - P. Szomolanyi - F. Ciampor - M. Cihova - V. Repiska - K. Ondicova - B. Mravec - Č. Altaner

Detaily:

Rok, strany: 2016, 925 - 933

O článku:

We report on a simple iron oxide (Venofer) labeling procedure of dental pulp mesenchymal stem cells (DP-MSCs) and DP-MSCs transduced with yeast cytosinedeaminase::uracilphosphoribosyltransferase (yCD::UPRT-DP-MSCs). Venofer is a drug approved for intravenous application to treat iron deficiency anemia in patients. Venofer labeling did not affect DP-MSCs or yCD::UPRT-DP-MSCs viability and growth kinetics. Electron microscopy of labeled cells showed internalized Venofer nanoparticles in endosomes. MRI relativity measurement of Venofer labeled DP-MSCs in a phantom arrangement revealed that 100 cells per 0.1 ml were still detectable. DP-MSCs or yCD::UPRT-DP-MSCs and the corresponding Venofer labeled cells release exosomes into conditional medium (CM). CM from yCD::UPRT-DP-MSCs in the presence of a prodrug 5-fluorocytosine caused tumor cell death in a dose dependent manner. Iron labeled DP-MSCs or yCD::UPRT-DP-MSCs sustained their tumor tropism in vivo; intra-nasally applied cells migrated and specifically engrafted orthotopic glioblastoma xenografts in rats. Keywords: dental pulp MSCs, iron labeling, yCD::UPRT-exosomes, intranasal application, migration to intracerebral glioblastoma

Ako citovať:

ISO 690:

Altanerova, U., Benejova, K., Altanerová, V., Tyciakova, S., Rychlý, B., Szomolanyi, P., Ciampor, F., Cihova, M., Repiska, V., Ondicova, K., Mravec, B., Altaner, Č. 2016. Dental pulp mesenchymal stem/stromal cells labeled with iron sucrose release exosomes and cells applied intra-nasally migrate to intracerebral glioblastoma. In NEOPLASMA, vol. 63, no.6, pp. 925-933. 0028-2685.

APA:

Altanerova, U., Benejova, K., Altanerová, V., Tyciakova, S., Rychlý, B., Szomolanyi, P., Ciampor, F., Cihova, M., Repiska, V., Ondicova, K., Mravec, B., Altaner, Č. (2016). Dental pulp mesenchymal stem/stromal cells labeled with iron sucrose release exosomes and cells applied intra-nasally migrate to intracerebral glioblastoma. NEOPLASMA, 63(6), 925-933. 0028-2685.