In vitro and in vivo antitumor activity of a novel chlorin derivative for photodynamic therapy

Rok, strany: 2016, 37 - 43

In presented paper, a new chlorin derivative 5,10,15,20-tetrakis[(5-N-morpholino)pentyl] chlorin (TMC) was investigated as a photosensitizer in photodynamic therapy (PDT). Cellular uptake, cytotoxicity, intracellular location, biodistribution and antitumor effects were studied using human esophageal cancer cells (Eca-109) and human cervical cancer cells (Hela) in vitro and an esophageal cancer model in BALB/c nude mice. Cellular uptake and biodistribution of TMC were measured by fluorescence spectrophotometer. Cytotoxicity of TMC against Eca-109 and Hela cells was determined by MTT assay. The intracellular location of TMC was detected with a confocal microscopy. It was showed that TMC could rapidly accumulate in tumor cells and localize in cytoplasm. TMC was found to be low-toxic in dark but extensively photosensitive in vitro. A fast clearance rate of TMC was observed in Eca-109-bearing mice. In particular, TMC could significantly inhibit the tumor growth and exhibit a notable antitumor efficacy for PDT in vivo. Keywords: photodynamic therapy, photosensitizer, tumor, chlorin

ISO 690:

Zhang, C., Zhang, L., Li, J., Wu, Z., Chen, N., Yan, Y., Chen, Z. 2016. In vitro and in vivo antitumor activity of a novel chlorin derivative for photodynamic therapy. In NEOPLASMA, vol. 63, no.1, pp. 37-43. 0028-2685.

APA:

Zhang, C., Zhang, L., Li, J., Wu, Z., Chen, N., Yan, Y., Chen, Z. (2016). In vitro and in vivo antitumor activity of a novel chlorin derivative for photodynamic therapy. NEOPLASMA, 63(1), 37-43. 0028-2685.