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Induction of apoptosis by capsaicin in hepatocellular cancer cell line SMMC-7721 is mediated through ROS generation and activation of JNK and p38 MAPK pathways

In: NEOPLASMA, vol. 62, no. 4
H. Bu - K. Cai - F. Shen - X. Bao - Y. Xu - F. Yu - H. Pan - C. Chen - Z. Du - J. Cui
Detaily:
Rok, strany: 2015, 582 - 591
O článku:
Capsaicin, one of the major pungent ingredients found in red peppers, has been shown to have anti-carcinogenic effect on various cancer cells through multiple mechanisms. In this study, we investigated the apoptotic effect of capsaicin on human hepatocellular cancer cell line SMMC-7721, as well as the possible mechanisms involved. Treatment of SMMC-7721 cells with capsaicin resulted in a dose-dependent inhibition of cell-viability and induction of apoptosis which was associated with the generation of ROS and persistent disruption of mitochondrial membrane potential. These effects were significantly blocked when cells were pretreated with a general antioxidant N-acetyl cysteine (NAC). We also found that capsaicin induced JNK and p38 MAPK phosphorylation. JNK and p38 MAPK inhibitor effectively blocked capsaicin-induced SMMC-7721 cell apoptosis. In addition, NAC completely blocked phosphorylation of JNK and p38 MAPK induced by capsaicin. Our results indicate that capsaicin induced in SMMC-7721 cell apoptosis through generation of intracellular ROS and activation of JNK and p38 MAPK pathways. Keywords: capsaicin, hepatocellular cancer, apoptosis, ROS, JNK and p38
Ako citovať:
ISO 690:
Bu, H., Cai, K., Shen, F., Bao, X., Xu, Y., Yu, F., Pan, H., Chen, C., Du, Z., Cui, J. 2015. Induction of apoptosis by capsaicin in hepatocellular cancer cell line SMMC-7721 is mediated through ROS generation and activation of JNK and p38 MAPK pathways. In NEOPLASMA, vol. 62, no.4, pp. 582-591. 0028-2685.

APA:
Bu, H., Cai, K., Shen, F., Bao, X., Xu, Y., Yu, F., Pan, H., Chen, C., Du, Z., Cui, J. (2015). Induction of apoptosis by capsaicin in hepatocellular cancer cell line SMMC-7721 is mediated through ROS generation and activation of JNK and p38 MAPK pathways. NEOPLASMA, 62(4), 582-591. 0028-2685.