In: NEOPLASMA, vol. 61, no. 6
Q. Wang - H. Hui - H. Yang - H. Li - Y. Gao - Z. Li - Q. Guo - N. Lu
Detaily:
Rok, strany: 2014, 647 - 658
O článku:
Our previous study investigated the effects of differentiation inducted by flavonoids derived from a Chinese herb. In this study, we found that LW-218, a new synthesized flavonoid, inhibited proliferation and induced differentiation of acute myeloid leukemia cells. The IC50s of LW-218 in HL-60, U937, K562, and NB4 cell lines were all less than 5 μM, suggesting greater capacity than compounds we have reported. LW-218 induced differentiation effects including morphologic changes, NBT reduction, and both of CD11b and CD14 expression. Results of western blots and siRNA transfection revealed that LW-218 increased the LAP/LIP ratio of C/EBPβ which regulated monocytic differentiation of leukemia cells. Meanwhile, these differentiation effects could be attenuated by silencing PLSCR1 via siRNA transfection. In addition, regulation on LAP/LIP ratio, of C/EBPβ was properly mediated by PLSCR1 which was up-regulated by LW-218. All these results suggested that C/EBPβ was involved in regulation of PKCδ/PLSCR1 pathway during flavonoids-induced differentiation. LW-218 was a prospective differentiation inductor of AML cells and was requisite to proceed further investigation. Keywords: flavonoid, acute myeloid leukemia, differentiation, PLSCR1, C/EBPβ
Ako citovať:
ISO 690:
Wang, Q., Hui, H., Yang, H., Li, H., Gao, Y., Li, Z., Guo, Q., Lu, N. 2014. Involvement of C/EBPβ in monocytic differentiation of acute myeloid leukemia cells induced by LW-218, a new synthesized flavonoid. In NEOPLASMA, vol. 61, no.6, pp. 647-658. 0028-2685.
APA:
Wang, Q., Hui, H., Yang, H., Li, H., Gao, Y., Li, Z., Guo, Q., Lu, N. (2014). Involvement of C/EBPβ in monocytic differentiation of acute myeloid leukemia cells induced by LW-218, a new synthesized flavonoid. NEOPLASMA, 61(6), 647-658. 0028-2685.