Facebook Instagram Twitter RSS Feed Back to top

The list of national projects SAS

Centre of Biosciences SAS
Alternative parasite control of small ruminant
Alternatívne terapie parazitóz malých prežúvavcov
Program: SRDA
Project leader: MVDr. Váradyová Zora PhD
Annotation:Diseases caused by helminths of farm animals are among the most serious infectious diseases. They account for over 55% of all livestock diseases in Europe and cause significant losses in animal production figures. The spread of anthelmintic resistance in livestock parasites has led to the failure of therapy and the need to develop new strategies and recommendations for effective control of the antihelminthic parasite resistance. The project offers an alternative strategies to currently used chemotherapy solutions, namely the concept of herbal nutraceuticals as feed for ruminants with an effect against gastrointestinal parasitoses.
Duration: 1.7.2019 - 30.6.2023
Analyses of allele-specific regulation of CD33 expression
Analýza alelovo-špecifickej regulácie expresie CD33
Program: VEGA
Project leader: Mgr. Královičová Jana PhD.
Duration: 1.1.2018 - 31.12.2021
Bacteriocins and their use to reduce spoilage microbiota in animal husbandry
Bakteriocíny a ich využitie na redukciu nežiaducej mikrobioty v chove zvierat
Program: VEGA
Project leader: MVDr. Pogány Simonová Monika PhD
Annotation:Methicillin resistance in staphylococci has had problem occuring also in animal production. Reduction and/or elimination of methicillin resistant (MetR) staphylococci in healthy breed is problem due to their multiresistance. However, utilization of antimicrobial proteinaceous substances –enterocins with a broad inhibition spectrum (produced by some strains of the genus Enterococcus) could be a promising way. In this basic research project will be therefore investigated inhibition activity of enterocins (characterized at our workplace) against a terget of faecal microbiota with impact on MetR and biofilm-forming staphylococci from various animals. Testing will be provided under in vitro as well as in vivo conditions; effect of enterocins against selected MetR strains will be verified in a model experiment by using experimental (mice) or farming (broiler rabbit) animals.
Duration: 1.1.2021 - 31.12.2024
Behavioural and physiological indicators of poultry welfare
Behaviorálne a fyziologické indikátory welfaru hydiny
Program: SRDA
Project leader: RNDr. Košťál Ľubor CSc.
Annotation:Factors influencing animal welfare include physical environment and resources available to the animal (resource-based indicators) as well as farm management practices (management-based indicators). Depending on its characteristics (breed, sex, age, etc.), the animal responds to these inputs and its responses are evaluated using animal-based welfare indicators. The EU funded project Welfare Quality®, as well as the EFSA's scientific opinions, have provided the toolbox of animal-based welfare indicators for various livestock species, including poultry. Nevertheless, there is still need to develop more specific indicators and new methods for measuring the established indicators. The aim of the project is to help with solving the problem of damaging behaviour in group housed laying hens without the need of painful beak-trimming, to contribute to the understanding of the relation between cognition and emotions in poultry, to assess the possibility of affecting welfare status by epigenetic effects of maternal androgens using Japanese quail lines selected for testosterone content in eggs as a model, to study the epigenetic effects of light quality during prenatal and early postnatal period on welfare, to analyse the possibility of using the immunological indicators of welfare status and to validate laboratory procedures for the analysis of corticosterone and androgen metabolites in poultry droppings, that could serve as a non-invasive welfare indicator.
Duration: 1.8.2018 - 30.6.2022
Bioactive oils in the therapy of microbial infections on avian chorioallantoic membrane
Bioaktívne oleje v terapii mikrobiálnych infekcií na modeli aviárnej chorioalantoickej membrány
Program: VEGA
Project leader: Mgr. Máčajová Mariana PhD.
Annotation:The treatment of various microbial and yeast infections is currently a major challenge in veterinary medicine. For breeders, some diseases such as mastitis, trichophytia or onychomycosis are often eradicating. Because of frequent antibiotic resistance, other treatments are emerging. In our project we will develop an in vivo model for the research of yeast and bacterial infections. We will use quail and turkey chorioallantoic membrane (CAM) to test the effects of various drugs and treatments. After validation and optimization of the model, we will topically apply bioactive substances in various concentrations and monitor their antimicrobial and biological effect. We will also monitor the additive effect of PDT using curcumin and hypericin. Angiogenic changes, expression of selected genes, histological and immunohistochemical changes after application of the substances will be examined. The obtained results will be useful in the treatment of yeast and microbial infections in animals and humans.
Duration: 1.1.2021 - 31.12.2024
Nutritional and health biomarkers in farm animals
Biomarkery nutričného a zdravotného statusu hospodárskych zvierat
Program: VEGA
Project leader: MVDr. Grešáková Ľubomíra PhD.
Annotation:Proposal project is focused on the investigation of appropriate dietary and health biomarkers reflecting the dietary intake, nutritional status and biological effects of feed additives used in animal nutrition. Nutritional biomarkers are biochemical, functional, or clinical indices of animal status that reflect nutrient exposure (recent or long term), nutritional status depending on digestibility, absorption and utilisation of dietary nutrient, and nutrient metabolism/effect in animal body. Effective gastrointestinal functionality is crucial in determining animal health, welfare and performance, therefore we will focus on the biomarker determination of gastrointestinal functionality. The suitability of selected biomarkers will be tested in experiments by animal feed supplementation. The experiments will be aimed to improve the mineral status and optimize digestive processes in poultry and sheep to minimize the environmental pollution by the reduction of methanogenesis and excretion of heavy metals.
Duration: 1.1.2021 - 31.12.2024
Bioavailability of zinc in animals - the possibilities and solutions
Biovyužiteľnosť zinku u hospodárskych zvierat - možnosti a riešenia
Program: SRDA
Project leader: RNDr. Čobanová Klaudia PhD.
Annotation:The objectives of the presented project proposal are aimed at obtaining a new knowledge about the bioavailability of zinc from various prevalently organic sources currently used as feed additives in animal nutrition. We will study the impact of plant secondary metabolites, fiber type and phytase activity in the diet on zinc absorption, retention and tissue deposition in ruminants and monogastric animals. At the same time, we will examine the effect of organic zinc and herbal nutraceuticals on the physiological processes of the gastrointestinal tract in in vitro and in vivo conditions and the antioxidant status of animals. Our principal intention will be to enhance the potentials of an efficient use of mineral additives in animal nutrition and thus contribute to the reduction of the negative impact of animal husbandry on the environment.
Duration: 1.8.2018 - 30.6.2022
-
Diagnostika onkologických ochorení pomocou aptasenzorov: vývoj a validácia
Program: VEGA
Project leader: Ing. Poturnayová Alexandra PhD.
Duration: 1.1.2021 - 31.12.2024
Etiology of early preimplantation development disorders
Etiológia porúch skorého preimplantačného vývinu
Program: SRDA
Project leader: MVDr. Fabian Dušan DrSc.
Annotation:Decreasing rates of early embryo survival after sucessfull fertilization cause economic losses in animal production. Embryo survival depends on the extent of induced disorders and ability of embryo to repair them. Using mouse model, the project will study molecular mechanisms by which selected exogenous (environmental burden) and endogenous factors (disordered maternal milieux) cause preimplantation development failure. Signaling pathways activated by specific ligands originating from environment and from maternal organism will be analyzed. Outcomes will elucidate mechanisms of developmental disorders induced by insecticides with embryotoxic potential and by maternal obesity. Collaterally, project will assess the effectivity of two basic reparatory mechanisms in preimplantation embryos: elimination of damaged cells and repair of damaged DNA. Results will bring new information on the physiological abilities of early embryonic cells to cope with externally induced disorders. Findings should help with solving problem of gradually increasing incidence of post-conceptional infertility.
Duration: 1.7.2019 - 30.6.2023
Functional analysis of new proteins required for meiotic recombination.
Funkčná analýza nových proteínov potrebných pre meiotickú rekombináciu
Program: VEGA
Project leader: Mgr. Bágeľová Poláková Silvia PhD.
Annotation:Sexual reproduction is primarily dependent on meiosis, a specialized cell division that generates haploid cells from diploid progenitors. It is absolutely essential that the halving of chromosome number is achieved accurately, because errors in this process can lead to genetic diseases such as Down Syndrome. The aim of our research project is to understand the molecular function of new proteins required for meiotic chromosome segregation in the yeast Schizosaccharomyces pombe. To identify new proteins required for meiotic chromosome segregation, we screened S. pombe deletion mutant library. We have identified 25 new proteins, which have not yet been implicated in meiosis. The functional analysis of three of these proteins Dbl2, Mal3 and Mto1 has been recently published (Polakova et al., 2016; 2014). The results of this screening demonstrate that many of the key regulators of meiotic chromosome segregation have not yet been described, and thus open exciting possibilities for future research projects.
Duration: 1.1.2019 - 31.12.2021
Functional food based on goats milk and its benefit on health
Funkčné potraviny na báze kozieho mlieka a ich prospešnosť pre zdravie
Program: SRDA
Project leader: MVDr. Lauková Andrea CSc.
Annotation:Functional food, can be characterized as common and/or traditional food enriched with special component;component will allott to that that food term functional meaning immunity stimulation, tracy elements addition to the body following the principal aim to have beneficial effect on health. Relating to dairy industry or agriculture, nowadays is prefering goats milk and/or products made up from it. In the framework of functional food is very popular and acceptable involving of probiotic bacteria; after their colonization in host organism, they can form ideal environment which with their metabolites or with their involving in the receptors influencing immune system can beneficially influence host organism.These bacteria can reach host organism/consumer by two ways: per os or via food. The project is focused on isolation, identification, general and technological characterization of autochtonnous lactic acid bacteria from goats milk and cheese of different Slovak producers; suitable, selected strain will be applied into product made up of goats milk in semi-processing (laboratory) conditions as well as in dairy-farming – customer of project application output condition; then the application form and effect of that one functional food will be tested using volonters (via consumption of cheese or the other products made up of goats milk). Application output will be the authochtonnous culture which could be used for technological processing of goats milk and/or products made up of it; these together with their final benefit on cunsumer health could allottthat product to functional food.
Duration: 1.8.2018 - 31.7.2022
-
Charakterizácia biosyntetickej dráhy kardiolipínu kvasinky Schizosaccharomyces pombe ako modelovej eukaryotickej bunky
Program: VEGA
Project leader: RNDr. Griač Peter CSc.
Annotation:Fission yeast Schizosaccharomyces pombe represents an important unicellular model organism to study molecular and cell biology of eukaryotes. Our knowledge of mitochondrial lipid biogenesis of this yeast is only very limited. That prevents the use of S. pombe as a model to study mitochondrial disease mechanisms and as a tool to discover therapies. Goal of the proposed project is characterization of the biosynthetic pathway of a unique mitochondrial phospholipid, cardiolipin (CL). We will analyze enzymes of this pathway with the focus on CL synthase, phosphatidylglycerolphosphate (PGP) synthase, PGP phosphatase as well as enzymes responsible for CL remodeling. We will study regulation of CL biosynthetic pathway on transcriptional and posttranscriptional levels. We will compare CL biogenesis in S. pombe, Saccharomyces cerevisiae and higher eukaryotes to evaluate the suitability of S. pombe as a unicellular eukaryotic model to study mitochondrial processes.
Duration: 1.1.2019 - 31.12.2022
Characterization of new yeast model of Barth syndrome.
Charakterizácia nového kvasinkového modelu pre štúdium Barthovho syndrómu.
Program: VEGA
Project leader: Mgr. Balážová Mária PhD.
Annotation:Barth syndrome (BTHS) is a serious hereditary mitochondrial disorder characterized by a decrease in total cardiolipin (CL) and in accumulation of its precursor monolyso-CL due to the loss of transacylase tafazzin. The molecular basis underlying the pathology of BTHS is still not well understood. The aim of this project is characterization of a new yeast model of BTHS (pgc1taz1) and its validation for the study of the role of different lipids, phospholipids and sphingolipids, on mitochondrial morphology, function, and reactive oxygen species production. Yeast model system will highly enhance the capacity to identify molecular mechanism how changes in membrane lipids lead to defects in mitochondrial functions and morphology not only in BTHS but also in other mitochondrial disorders.
Duration: 1.1.2018 - 31.12.2021
Interactions of mitochondrial genomes
Interakcie mitochondriálnych genómov
Program: VEGA
Project leader: Mgr. Bágeľová Poláková Silvia PhD.
Annotation:Evolution and the origin of new species can be studied experimentally in yeasts, where new species arise in the process of speciation from interspecific hybrids. Hereby the crucial role play “out of tune” nucleo-mitochondrial interactions. Our aim is to use a number of hybrids with recombined mitochondrial DNA (mtDNA) and known sequence to complete the aims from previous projects as well as to elucidate: (i) nucleo-mitochondrial interactions in interspecific hybrids; (ii) the nature of gain-of-function mutations that allow the cell to adapt to foreign mtDNA; (iii) whether mitochondrial protein synthesis is required to maintain complete mitochondrial genomes; (iv) to obtain evidence for the species-specific gene order in mtDNA and for the existence of recombined mtDNAs in nature; (v) to investigate the possibility of rearranging of the gene order in mtDNA; (vi) to investigate the possibility of communication in yeast mitochondria through small proteins encoded by the genes for mitochondrial ribosomal RNAs.
Duration: 1.1.2020 - 31.12.2023
-
Kontrola integrity DNA v preimplantačnom embryu
Program: VEGA
Project leader: MVDr. Baran Vladimír CSc.
Duration: 1.1.2019 - 31.12.2021
-
Kotranskripčné formovanie pre-mRNA štruktúry, model štrukturálnych motívov nevyhnutných pre definíciu exónu
Program: SRDA
Project leader: Mgr. Královičová Jana PhD.
Duration: 1.7.2019 - 30.6.2023
The skin microbiome in dogs under physiological and pathological conditions
Kožný mikrobióm psov za fyziologických a patologických podmienok
Program: VEGA
Project leader: MVDr. Strompfová Viola DrSc.
Annotation:The complex community of microorganisms involved in important physiological functions, such as the biosynthesis of vitamins and amino acids or modulation of immune responses, is present not only in the gastrointestinal tract but also on the surface of intergumentary system in both humans and animals. Seeing that it is organ continually exposed to environmental conditions, many factors can contribute to the development of dysbiosis leading to various dermatological problems very frequent in dogs nowdays. However, knowledge about the composition, diversity and functions of skin microbiota in dogs is negligible. The aim of this project will be the taxonomic characterization of skin microbiota detected using cultivation and next-generation sequencing, focusing on healthy individuals and individuals with various skin diseases predominantly of infectious and immune character. The possibilities of optimizing/stabilizing bacterial skin colonization for maintenance of physiological skin functions will also be tested.
Duration: 1.1.2020 - 31.12.2023
Cryopreservation of cattle gametes and embryos for gene banking
Kryouchovávanie gamét a embryí hovädzieho dobytka pre účely génovej banky
Program: SRDA
Project leader: RNDr. Antalíková Jana PhD.
Annotation:Cryopreservation and subsequent long-term preservation of gametes from genetically significant individuals of different livestock breeds play an important role in solving the issue of preserving animal gene resources. The methods of freezing of oocytes and ovarian tissues must ensure their good quality and viability after thawing. In our previous studies, we tested the in vitro cryopreservation procedure of mature bovine oocytes, which proved to be more successful compared to freezing of ovarian fragments containing follicles with immature oocytes. However, due to the high variability of results after in vitro fertilization of thawed oocytes, this strategy requires further optimization. The project will be focused on increasing the survival of oocytes after thawing and minimizing freezing damages to oocytes, as well as on promoting cell division and development of preimplantation embryos in vitro derived from frozen oocytes. In this concern, besides optimizing the cryopreservation regime, we plan to modify the culture conditions by using special additives to the culture media (antioxidants, growth factors, etc.). It is expected that optimization of the methods would ensure greater cryotolerance of bovine oocytes and in vitro produced embryos for gene bank preservation purposes.
Duration: 1.7.2020 - 30.6.2024
Punicic acid: production and mechanisms of its action in yeasts
Kyselina puniková: produkcia a mechanizmy jej účinku v kvasinkách
Program: VEGA
Project leader: Mgr. Holič Roman PhD.
Annotation:Punic acid (18: 3Delta9cis, 11trans, 13cis) is the major constituent of triacylglycerols in pomegranate seed oil. It is characterized by a wide range of bioactive effects including currently highly attractive anti-cancer properties. Mechanisms of its action are unknown. The project is focused on the examination of its heterologous production and lipotoxicity in yeasts. Punicic acid production will be achieved by heterologous expression of two pomegranate genes involved in its biosynthesis. Project will focus on distribution of punicic acid in the cell, its effect on cell division, and mechanisms facilitating its efficient storage in the form of storage lipids. The acquired knowledge will contribute to the elucidation of anticancer effects of punicic acid and identification of factors necessary for its overproduction in yeast. Obtained results can be applied also in biotechnological production of other unusual fatty acids in microorganisms.
Duration: 1.1.2020 - 31.12.2023
Mechanisms of communication and adaptation of preimplantation embryo in a disturbed environment
Mechanizmy komunikácie a adaptácie preimplantačného embrya v narušenom prostredí
Program: VEGA
Project leader: RNDr. Čikoš Štefan DrSc.
Annotation:Maternal physiological condition and environmental factors can significantly influence preimplantation embryo development. Disturbances at this developmental period can have long-term consequences, including unsuccessful gravidity or impaired offspring health, and can cause economic losses in animal production. The proposed project will study mechanisms involved in the communication of early mammalian embryo with the surrounding environment and responses enabling the early embryo to adapt to changes in its environment. Expression of specific types of cell receptors (especially receptors binding small-molecule ligands) in preimplantation embryos and effects of their activation on the embryo development, especially on the cell survival and apoptosis will be studied. Mechanisms of dead cell elimination in preimplantation embryo will be studied as well. Results obtained in the mouse model will be verified in selected farm animals to identify conserved mechanisms or to reveal possible inter-species differences.
Duration: 1.1.2019 - 31.12.2022
Voltage dependent calcium channels in nociception.
Napäťovo závislé vápnikové kanály v nocicepcii
Program: VEGA
Project leader: prof. RNDr. Lacinová Ľubica DrSc.
Duration: 1.1.2019 - 31.12.2021
-
Nová generácia antidepresív - dlhodobé účinky na potomstvo
Program: SRDA
Project leader: prof. RNDr. Lacinová Ľubica DrSc.
Duration: 1.7.2020 - 30.6.2024
New generation of feed additives in animal nutrition.
Nova generácia kŕmnych aditív vo výžive zvierat.
Program: VEGA
Project leader: MVDr. Plachá Iveta PhD
Annotation:Recently, we have recorded a large number of scientific publications dedicated to the use of phytoadditives in animal nutrition. In particular those containing phenolic constituents with strong antioxidative properties. Most of the studies examined particularly the production performance. Whereas majority of scientific works is devoted to the commercial use only limited number of scientifically verified works is focused on the mechanism of action and metabolism of plant compounds. Based on our current knowledge we set a goal to research the metabolic biotransformation of the main bioactive component of Thymus vulgaris, thymol. The obtained results will enhance our current knowledge regarding its absorption and distribution in animal organism. The wide scientific community will be enriched by the results concerning the mechanism of action of main compounds of plant feed additives in animal organism as these knowledges are still lacking in scientific literature.
Duration: 1.1.2020 - 31.12.2023
-
Nový pohľad na izotiokyanáty ako látky zasahujúce do homeostázy neoplasticky transformovaných buniek
Program: VEGA
Project leader: RNDr. Imrichová Denisa PhD.
Duration: 1.1.2021 - 31.12.2024
Unravelling the mechanisms of post-translational regulation of RNA splicing factors in maintenance of genome integrity
Objasnenie mechanizmov posttranslačnej regulácie faktorov zostrihu RNA pri udržiavaní stability genómu
Program: SRDA
Project leader: Mgr. Bágeľová Poláková Silvia PhD.
Annotation:Several mutations in RNA biogenesis factors have been implicated in human cancers. Early studies linked RNA processing defects with genome instability phenotypes such as hyper-mutation and hyper-recombination. Since then, recent functional genomic studies have implicated several more aspects of RNA processing in genome instability and revealed that virtually every major aspect of RNA processing is potentially mutable to a genome instability phenotype and in some cases are coupling RNA processing defects to increased RNA:DNA hybrid- mediated R-loop formation, which in turn constitute a major source of genome instability across species. Despite it is now clear that RNA processing defects could destabilize genomes, the molecular mechanism of post-translational regulations of RNA processing and its connections to genome instability are not clear. Our project is aimed at a detailed analysis of the regulatory role of phosphorylation for functions of splicing factors that co-purified with the recently identified spliceosome-associated Nrl1 protein of fission yeast S. pombe. We showed Nrl1 protein be involved in suppression of accumulation of genome threating RNA:DNA hybrids, structures formed during RNA processing. The experimental approaches include protein purifications and phospho proteomics analysis, which will help us to identify post-translational modifications of splicing factors. Analysis of phenotypes of phospho mutants of splicing factors employ fluorescence/live-cell microscopy, analysis of splicing defects of these mutants using RT-PCR or transcriptome sequencing, followed by analysis of their sensitivity to DNA damaging agents and analysis of their defects in DNA repair pathways. The obtained data should bring especially completely new information concerning the regulatory roles of post-translational modifications associated with the defects of RNA processing leading to genome instability that may be important as possible targets for anti-cancer therapy.
Duration: 1.7.2017 - 30.6.2021
Defense mechanisms of microbial and animal cells in reducing their sensitivity to plant defensive compounds
Obranné mechanizmy mikrobiálnych a živočíšnych buniek pri znižovaní ich citlivosti na rastlinné defenzné zlúčeniny
Program: SRDA
Project leader: Ing. Sulová Zdena DrSc.
Annotation:Living organisms have developed during evolution different strategies for securing better position in the competition for vital sources. Biosynthesis of various biologically active substances enabled specific organisms to be more successful than others. Plant metabolites have important position among these substances. Many products of plant secondary metabolism are known as effective pharmacological tools. A widespread use of these plant products can be predicted in various applications in veterinary and human medicine, food safety, etc. These applications will depend on the development of knowledge in biosciences, to which the presented project aims to contribute. The ability to modify the structure of known substances of plant origin in the laboratory gives almost unlimited possibilities for the rational design of new biologically active structures. Therefore, we choose "OMNIPOTENCE" as the acronym of our project. The project is aimed primarily on several plant products and their semisynthetic analogues as possible tools for modulation of proliferation and phenotypes of neoplastic and microbial cells. Living cells have developed several defensive strategies against toxic attacks mediated by different substances. The ability to develop such strategies may be considered as a basal characteristic that enables the cell to survive in the hostile environment. In addition to response specific for various organisms, general strategies have developed during evolution that are functional in various types of living cells including mammalian neoplastically transformed cells, eukaryotic and prokaryotic microbial cells. The proposed project will focus on detailed analysis of the mechanisms how neoplastic mammalian and microbial cells respond to selected plant defensive substances and their semisynthetic derivatives.
Duration: 1.7.2020 - 30.6.2024
A pilot study of the selective effects of a new generation of RNA interfering agents at the cellular level
Pilotná štúdia selektívneho pôsobenia novej generácie RNA interferenčných agens na bunkovej úrovni
Program: VEGA
Project leader: Ing. Poturnayová Alexandra PhD.
Duration: 1.7.2020 - 31.12.2022
Brain plasticity associated with learned vocal communication in songbirds
Plasticita mozgu v spojení s naučenou vokálnou komunikáciou u spevavcov
Program: VEGA
Project leader: Mgr. Niederová-Kubíková Ľubica PhD.
Annotation:Neuroplasticity or brain plasticity is the ability of the brain to change as a result of experience or injury. Except of other factors, neuroplasticity is enabled by neurogenesis. Impaired neurogenesis is associated with cognitive decline, behavioral changes, depression, and various neurodegenerative diseases. One of the aspects that can influence neurogenesis, and thus learning, memory or the number of new neurons in an injured area, is the circadian clock. However, its presence in various species is not clearly established. Therefore, our first aim is to determine if there are circadian oscillations in the number of newborn cells in the brain of songbirds. Songbirds share with humans some traits of neurogenesis as well as the rare ability of vocal learning. Injury in one of the areas controlling song led to changes in cerebellum that has been not studied for vocal learning up to now. Thus, in the second part of the project we propose to study the function of cerebellum for the learned vocal communication.
Duration: 1.1.2020 - 31.12.2023
Keel bone damage – causes and consequences for laying hens welfare, health and production
Poškodenia hrebeňa hrudnej kosti – príčiny a dôsledky pre welfare, zdravie a produkciu nosníc
Program: VEGA
Project leader: RNDr. Bilčík Boris PhD.
Annotation:Keel bone damage is currently one of the major problems influencing welfare of laying hens. Extremely high frequency and severity of damage adversely affects also egg quality and production. The aim of the present project is to investigate the prevalence of the keel bone damage in various types of housing technologies in Slovakia, to apply innovative methods of fracture and deformation detection and to test the possibilities of intervention in breeding, technology and management.
Duration: 1.1.2019 - 31.12.2022
Sterol quality control in utilisation of external sterols under hypoxic stress in yeast
Príjem a metabolizmus externých sterolov počas hypoxického stresu u kvasiniek
Program: VEGA
Project leader: Mgr. Valachovič Martin PhD.
Annotation:Hypoxic stress significantly affects sterol homeostasis in yeast. The proposed project addresses utilization of exogenous sterols, namely animal cholesterol and plant sterols, as an adaptation to low oxygen conditions. We will focus on the mechanism(s) that cells possess to monitor compatibility of imported sterols and yeast membranes. We named this a ‘sterol quality control‘ concept. Studied will be individual components of sterol import: cell wall proteins, plasma membrane sterol transporters and sterol esterifying enzymes. In a view of the proposed concept, the physical and physiologically relevant membrane properties in which native ergosterol has been replaced by environmental sterols will be analyzed. Since the host sterol uptake contributes to resistance of pathogenic fungi to antimycotics, the project has the potential with respect to the innovation of existing antimycotics as well as development of the new ones.
Duration: 1.1.2020 - 31.12.2023
Behavioural disturbances in poultry – influence of mother presence and definition of individual phenotypes susceptible to its development
Problémové správanie hydiny – vplyv prítomnosti matky a definovanie individuálnych fenotypov náchylných na jeho vývin
Program: VEGA
Project leader: Mgr. Pichová Katarína PhD.
Annotation:The poultry industry and especially egg industry in Europe undergoes significant changes. The primary objective of these changes is to improve animal welfare, quality of life of animals. Despite of the efforts producers have to face large economical and welfare problems such as feather pecking, cannibalism and aggressive behaviour. There is increasing pressure to solve these problems in a way friendly towards the animals that will not cause additional stress or pain. The aim of the project is to extend information on damaging behaviour in poultry and to contribute to the understanding of the factors influencing a development of this behaviour, such as the influence of rearing with mother or the impact of individual differences, personality, susceptibility to the development of disturbances, and in cooperation the European network of scientists to propose solutions feasible for agricultural practice.
Duration: 1.1.2019 - 31.12.2022
Dbl2 protein as a novel regulator of genome stability and dynamics in fission yeast
Proteín Dbl2 ako nový regulátor stability a dynamiky genómu v kvasinkách Schizosaccharomyces pombe
Program: SRDA
Project leader: Mgr. Bágeľová Poláková Silvia PhD.
Annotation:Sexual reproduction is primarily dependent on meiosis, a specialized cell division that generates haploid cells from diploid progenitors. To identify novel regulators of meiosis, we screened a library of fission yeast deletion mutants and found that deletion of the dbl2 gene led to missegregation of chromosomes during meiosis. Analysis of live dbl2Δ cells by fluorescence microscopy showed that chromosomes frequently failed to segregate during meiotic or mitotic division. Further cytological and biochemical analyses revealed that this segregation defect is due to persistent intermediates of DNA double-strand break repair, also called DNA joint molecules. Our results indicate that Dbl2 is required for formation of Fbh1 DNA helicase foci at the sites of DNA double-strand break repair in order to process DNA joint molecules and allow segregation of chromosomes during meiotic or mitotic divisions. Our new data suggests that in addition to performing a function in meiotic recombination and DNA repair, Dbl2 is also a component of a genome mechanism that is used to decrease the levels of both protein- coding and non-coding RNAs. This study intends to determine the molecular mechanism by which Dbl2 regulates genome stability and gene expression. We will use classical genetic and biochemical methods, advanced microscopy, RNA and CHIP-sequencing as well as reconstitution of hypothetical cell biological events in vitro to uncover its function. Importantly, the findings of this research will have broad implications to the field of sexual reproduction and cancer research.
Duration: 1.7.2019 - 30.6.2022
Regulation of the Swi5-Sfr1 complex by protein phosphorylation.
Regulácia komplexu Swi5-Sfr1 pomocou fosforylácie.
Program: SRDA
Project leader: Mgr. Bágeľová Poláková Silvia PhD.
Annotation:Homologous recombination is important for repair of damaged DNA in vegetative cells but is also required for proper segregation of chromosomes during meiosis, a specialized cell division, which produces haploid gametes such as eggs and sperm cells in mammals and spores in yeast. Swi5-Sfr1 heterodimer complex stimulates the Rad51-mediated exchange of DNA strands, a key step in homologous recombination. We have identified swi5 and sfr1 deletion mutants in a screening for mutants defective in chromosome segregation during meiosis in the fission yeast Schizosaccharomyces pombe. Our initial characterization revealed new phosphorylation sites on both Swi5 and Sfr1. In this project, we want to decipher how phoshorylation regulates the role of Swi5 and Sfr1 in both vegetative and meiotic S. pombe cells. This will help us to understand the process of homologous recombination and reduction of chromosome number during meiosis. Both Swi5 and Sfr1 proteins are evolutionarily conserved, raising the possibility that our results will be relevant to studies in various organisms including mammalian cells.
Duration: 1.7.2018 - 30.6.2022
Regulation of ryanodine receptors (RYR) from the rat brain under physiological and pathophysiological conditions
Regulácia ryanodínových receptorov (RYR) z mozgu potkana vo fyziologických a patofyziologických podmienkach
Program: VEGA
Project leader: Mgr. Gaburjáková Marta PhD.
Annotation:The ryanodine receptor (RYR) plays a key role in neuronal Ca2+ signaling that is substantially involved in neurotransmission. In the brain, all three RYR isoforms are expressed that likely complicates study of their function and regulation at the single channel level. Our aim is to reliably recognize individual RYR isoforms using specific functional parameters and assess whether there exist tissue-specific properties of them. Further, we will monitor RYR channels from the rat brain under pathophysiological conditions that will be achieved by stripping FKBP12 (the RYR associated protein) from the channel complex employing the endogenous phosphorylation machinery. For the RYR2 isoform (the dominant one in the brain), we will test our hypothesis about the inhibition effect of dantrolene, which is likely manifested only under some conditions. Our ambition is to understand the molecular mechanism of the therapeutic effect of dantrolene on some neurodegenerative diseases that are linked to RYR dysregulation.
Duration: 1.1.2020 - 31.12.2023
-
Sledovanie prepojenia molekulárnych dejov vedúcich k rozvoju viacliekovej rezistencie v leukemických bunkách
Program: VEGA
Project leader: Ing. Sulová Zdena DrSc.
Duration: 1.1.2021 - 31.12.2024
Study and use of the bacteriocins in prevention of American foulbrood
Štúdium a využitie bakteriocínov v prevencii moru včielieho plodu
Program: VEGA
Project leader: RNDr. Kopčáková Anna PhD.
Duration: 1.1.2019 - 31.12.2022
Investigation of the mechanism of action of novel putative antidepressant drugs: pyridoindole derivatives and trace amine-associated receptor-1 (TAAR1) ligands
Štúdium mechanizmov účinku nových antidepresív: pyridoindolové deriváty a ligandy „trace amine-associated receptor one“ (TAAR1)
Program: VEGA
Project leader: MMedSc Dremencov Eliyahu PhD
Annotation:The aim of the project will be to investigate the mechanism of action of the novel potential antidepressant drugs, such as pyridoindole derivatives and ligands of trace amine-associated receptor-1 (TAAR1). These drugs will be administered to the laboratory rats and their effects on their anxiety and depressive-like behavior and excitability of glutamate, serotonin, norepinephrine, and dopamine-secreting neurons in vivo and in neuronal primary cultures will be examined. This project will be performed in a collaboration with the industrial biopharmaceutical sector.
Duration: 1.1.2018 - 31.12.2021
Study of tetraspanin family proteins in cattle reproduction process
Štúdium proteínov tetraspanínovej rodiny v procese reprodukcie hovädzieho dobytka
Program: VEGA
Project leader: Ing. Jankovičová Jana PhD.
Annotation:Tetraspanins are membrane proteins of various types of somatic cells of many animal species involved in numerous functions. Based on the described expression and functional experiments on mouse gametes, it is clear that they are also involved in the fertilization process. Our analyses confirmed the presence of CD9 and CD81 tetraspanins on bull sperm as well as bovine and porcine oocytes and embryos. Since these proteins act within the complex, so-called tetraspanin web, the aim of this project is to study other molecular partners, whether from the family of tetraspanins or others in terms of their importance in the process of bovine reproduction. The results obtained would not only extend the knowledge in the field of physiology of mammalian reproduction, but could also contribute to the identification of new markers useful in practice, as the reproduction of livestock is an exclusively controlled process.
Duration: 1.1.2020 - 31.12.2023
The role of protein-protein interactions in regulation of the cardiac ryanodine receptor
Úloha proteín-proteínových interakcií v regulácii srdcového ryanodínového receptora
Program: VEGA
Project leader: Mgr. Gaburjáková Jana PhD.
Annotation:Ca2+ ions, essential for contraction of cardiomyocytes, are released from the sarcoplasmic reticulum (SR) via the cardiac ryanodine receptor (RYR2), an indispensable component of the excitation-contraction coupling machinery. Function of the RYR2 channel is modified by multiple associated proteins, located on both cytoplasmic and luminal RYR2 faces. The goal of this project is to investigate new aspects of protein-protein interactions involved in RYR2 regulation from the luminal side where calsequestrin (CSQ2) presumably senses and translates changes in luminal Ca2+ to the RYR2 channel. However, the RYR2 channel possesses also intrinsic luminal Ca2+ sensor within its first luminal loop. The physiological roles of such luminal sensors and their co-existence remain to be defined. We will examine what structural changes in CSQ2 induced by Ca2+ are communicated to the RYR2 channel and whether the regulation role of CSQ2 could be replaced by calreticulin, which is overexpressed in the SR in the absence of CSQ2.
Duration: 1.1.2021 - 31.12.2024
Multidrug resistance of leukemia cells - Phenotype caused by interference of multimodal molecular reasons
Viaclieková rezistencia u leukemických buniek - fenotyp spôsobený interferenciou viacerých molekulárnych príčin
Program: SRDA
Project leader: Ing. Sulová Zdena DrSc.
Annotation:The multidrug resistance (MDR) of neoplastic cells represents a serious problem in the treatment of cancer. Tumor transformed cells could develop a wide range of defense mechanisms that enable them to escape from apoptosis induced by chemotherapy. In our project, we will focus on research concerning the development and particularly identification of mechanisms involved in resistance of leukemic cells. The most common molecular feature responsible for development of MDR in neoplastic cells represent expressions and transport activity of transporters belonging from the ABC gene family, and particularly the best-known member of this family ABCB1 protein also known as P-glycoprotein (P-gp). Our findings in the previous period has shown that during development of multidrug resistance associated with P-gp overexpression several alterations of processes lead to cell death (such as: response to endoplasmic reticulum stress, deregulation of calcium dependent processes in the intracellular space or induction and progression of programed cell death – apoptosis) could occurred. These alterations are associated with the remodeling of surface proteins and their ubiquitination and glycosylation. These changes, and probably many others, create a complex insight of the molecular changes responsible for the reduced response of leukemia cells to the drugs induced apoptosis. In the current project we will focus to bring novel results important for understanding of the interplay of various molecular changes involved in development of multidrug resistance in leukemia cells. As a model we will use cell sublines obtained by adaption of parental leukemia cells to stressors of endoplasmic reticulum, proteasome inhibitors, hypomethylating agents and vincristine.
Duration: 1.7.2020 - 30.6.2024
Effect of endoplasmic reticulum-stress inducing substances on cell cycle regulatory mechanisms in cell lines with MDR phenotype
Vplyv látok vyvolávajúcich stres endoplazmatického retikula na regulačné mechanizmy bunkového cyklu buniek s viacliekovou rezistenciou
Program: VEGA
Project leader: Mgr. Šereš Mário PhD.
Duration: 1.1.2019 - 31.12.2021
The effect of polyphenols on the functions of signal proteins involved in proteins N-glycosylation in the endoplasmic reticulum of leukemia cells, depending on the expression of P-glycoprotein
Vplyv polyfenolov na funkcie signálnych proteínov zahrnutých v N-glykozylácii proteínov v endoplazmatickom retikule leukemických buniek v závislosti od expresie P-glykoproteínu
Program: VEGA
Project leader: RNDr. Boháčová Viera CSc.
Annotation:The ability of cells to withstand the action of substances with different structure and mechanism of action is called multidrug resistance - MDR. The aim of many studies is to understand mechanisms of MDR formation and ways to suppress it. P-glycoprotein (P-gp, member of the ABC transporter family) is probably the most commonly reported cause of MDR development in neoplastic cells. The transport function of P-gp may be affected by different groups of substances, e.g. polyphenols (quercetin). An important source of various natural polyphenols are green tea leaves (Camellia sinensis). Tea extract has healing effects, it contains different types of catechins, which are considered as bioactive substances. This study focuses on the influence of bioactive substances on the chaperons expression, supporting proteins needed for maturation, stability and maintenance of active proteins in cells. Furthermore, we will focus on the cell cycle and expression of transcriptional proteins p53 and p21.
Duration: 1.1.2018 - 31.12.2021
-
Vplyv vičenca vikolistého (Onobrychis viciifolia) na bachorovú fermentáciu, protozoálnu populáciu a hematologický profil u jahniat s hemonchózou
Program:
Project leader: Ing. Petrič Daniel
Duration: 1.1.2021 - 31.12.2021
Iron intake and antibiotic resistance in food animal Enterobacterales.
Vychytávanie železa a rezistencia na antibiotiká u Enterobacterales potravinových zvierat.
Program: VEGA
Project leader: RNDr. Bujňáková Dobroslava PhD
Annotation:The main objective of the proposed project will be to obtain a new knowledge about the relationship between antibiotic resistance, virulence, ferric ions uptake and biofilm formation on model species of food animal bacteria i. e. Enterobacterales and Escherichia concerning to extraintestinal - ExPEC, avian pathogenic - APEC and verotoxigenic – VTEC Escherichia coli, Klebsiella, Enterobacter. Research will be focused on the most important mechanisms of resistance: plasmid ampicillinases, cephalosporinases, carbapenemases, plasmid resistance to fluoroquinolones and resistance to colistin. Bacterial clonal relatedness and sequence type (ST) of selected Escherichia coli isolates will be also determined. The achieved results will be applicable in bacteriological diagnostic of resistance mechanisms by interpreting reading of minimal inhibitory concentration of antibiotic and in student education.
Duration: 1.1.2021 - 31.12.2024
Study of alteration in leukemia cells phenotype after induction of ABCB1 membrane transporter
Výskum zmien vo fenotype leukemických buniek po indukcii membránového transportéra ABCB1.
Program: VEGA
Project leader: prof. Ing. Breier Albert DrSc.
Annotation:The development of knowledge and methodology of biological sciences has provided opportunities for accurate analysis of genome, proteome, glycome and lipidome. This allows characterization of genotypic and phenotypic changes of patients and impact of these changes for choice of optimal therapeutic procedure and overall prognosis. Exact genotyping and phenotyping allows more successes in the design of treatment procedures also in patients with blood malignancies. However, multidrug resistance (MDR) may take part in chemotherapy of leukemia and represents a negative prognostic factor. The most frequent molecular cause of MDR is overexpression of membrane transporters from the ABC family (especially of P-glycoprotein, P-gp). P-gp in leukemic cells causes the development of MDR to its substrates. The expression of P-gp in leukemic cells is linked to extensive changes in protein profiles and their glycosylation that leads to altered leukemic cells phenotype. This phenomenon will be studied in the present project.
Duration: 1.1.2019 - 31.12.2022
The application of myrosinase for sulforaphane activation in development of a novel product exhibiting cancer prevention effects
Využitie myrozinázy na aktiváciu sulforafanu pre vývoj preparátu s preventívnymi účinkami nádorových ochorení
Program: SRDA
Project leader: Ing. Sulová Zdena DrSc.
Duration: 1.7.2017 - 30.6.2021
-
Vzťah mikroarchitektúry mitochondriálnej siete a jej funkčného spriahnutia v kardiomyocytoch
Program: VEGA
Project leader: Mgr. Macková Katarína PhD.
Duration: 1.1.2018 - 31.12.2021
-
Vzťah neurogenézy a spevu u spevavcov
Program:
Project leader: Mgr. Mertušová Justína
Annotation:New neurons are formed and integrated into brain regions not just in embryonal brains but also in adult brains of most vertebrates. Important yet still unknown is the function of neurogenesis. Songbirds represent a good model for understanding the neurogenesis function in adults as different neuronal cell types are continually incorporated into the brain areas controlling song learning, its perception and production. In some songbird species, higher neurogenesis is related to higher song variability. It has been proposed that newly generated neurons may facilitate behavioral variability. The aim of this study is to broaden the knowledge of this relationship among six other species belonging to different genus of the same songbird family Estrildidae. Monitoring the relationship between newly generated cells and/or neurons incorporated into the vocal areas and song variability parameters will bring a better understanding of the neurogenesis function as well as possible inter- and intra-species differences.
Duration: 1.1.2020 - 30.6.2021

The total number of projects: 48