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Institute: Institute of Molecular Biology

Raw starch degrading amylases - structure/function and evolution
Amylázy degradujúce surový škrob - štruktúra/funkcia a evolúcia
Program: Bilateral - other
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2012 - 31.12.2018

Amylolytic enzymes involved in degradation of starchy substrates
Amylolytické enzýmy zapojené do degradácie surového škrobu
Program: Bilateral - other
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2012 - 31.12.2018

Evolution of alpha-amylases and related enzymes
Evolúcia alfa-amyláz a príbuzných enzýmov
Program: Bilateral - other
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2015 - 31.12.2019

Rewiring the Streptomyces cell factory for cost-effective production of biomolecules
Nové prepojenie bunkovej továrne Streptomyces pre efektívnu produkciu biomolekúl
Program: FP7
Project leader: RNDr. Kormanec Ján DrSc.
Annotation:STREPSYNTH aims to set-up a Streptomyces-based new industrial production platform (SNIP) for high value added biomolecules. Streptomyces lividans was chosen as a bacterial host cell because it has been already shown to be highly efficient for the extracellular production of a number of heterologous molecules that vary chemically, has a robust tradition of industrial fermentation and is fully accessible to genetic intervention. To develop SNIP our strategy has two components: first, we will construct a collection of reduced-genome S. lividans strains. This will metabolically streamline the cell and rid it of agents (e.g. proteases) of potential harm to the heterologous polypeptides. Second, we will engineer synthetic parts and cassettes, i.e. reshuffled, rewired and repurposed genetic elements either indigenous to S. lividans or heterologous genes organized in artificial operon clusters. These elements will serve three aims: transcriptional and translational optimization, sophisticated on-demand transcriptional regulation that will provide unique fermentation control and metabolic engineering of complete cellular pathways channeling biomolecules to profuse extracellular secretion. Synthetic parts and cassettes will be either directly incorporated into the genome or be hosted in the form of plasmids. Systems biology tools will guide fine-tuning rounds of cell factory engineering and fermentation optimization. To set up SNIP we chose two classes of biomolecules with obvious immediate industrial value and application: heterologous proteins (industrial enzymes, biopharmaceuticals, biofuel enzymes, diagnostics) and small molecules (lantipeptides and indolocarbozoles) useful for multiple industrial purposes (biopharmaceuticals, additives, food technology, bioenergy). These biomolecules are of immediate interest to SMEs that participate and guide the industrial relevance of STREPSYNTH. SNIP is a modular platform that can be repurposed for diverse future applications.
Duration: 1.12.2013 - 30.11.2018

Protein design and evolution of raw starch digesting amylases
Proteínový dizajn a evolúcia amyláz degradujúcich surový škrob
Program: Bilateral - other
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2015 - 31.12.2019

Regulatory network of septin higher-order structures.
Regulačná sieť štruktúr vyššieho poriadku septínov.
Program: Bilateral - other
Project leader: RNDr. Farkašovský Marian CSc.
Annotation:Septins are conserved guanosine phosphate–binding proteins involved in cytokinesis and other essential membrane remodelling functions. They form heterooligomeric complexes, which polymerize into nonpolar filaments and are present in most eukaryotic organisms. Septin structures serve as a scaffold for the localized assembly of various proteins at the cell division plane. They also act as diffusion barrier for membrane-associated proteins, which generate different higher-order structures depending on the particular stage of the cell cycle. The mechanisms of formation, localization of septin structures and the regulation of septin ring assembly are difficult to study and therefore poorly understood. Our main goal is to explain how the protein kinases Elm1, Gin4, Cla4 and Cdc28 are involved in the regulation of coordinated assembly of septins in different stages of the cell cycle. We will study the in vitro phosphorylation of GFP-labelled septins by fluorescence microscopy observing the production of kinase reaction products in free solution and on supported lipid bilayer. Cryo electron microscopy and single particle analysis will be employed to obtain structural details of the phosphorylation-induced changes in septins. Observation of the localization and structural changes of the septin cortex in vivo as well as localization of interacting proteins at different conditions and in different genetic backgrounds (e.g. kinase mutants) will support our in vitro findings.
Duration: 1.1.2017 - 31.12.2019

Structure/function and evolution of amylolytic enzymes
Štruktúra, funkcia a evolúcia amylolytických enzýmov
Program: Bilateral - other
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2016 - 31.12.2020

Structure, function and evolution of trehalose synthases
Štruktúra, funkcia a evolúcia trehalózasyntáz
Program: Bilateral - other
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2015 - 31.12.2019

Development of structural methods to the study of pharmacologically active enzymes
Vývoj štruktúrnych metód na štúdium farmakologicky aktívnych enzýmov
Program: Multilateral - other
Project leader: RNDr. Urbániková Ľubica CSc.
Annotation:This project will establish the first structural biology consortium in the Danube region, creating a regional research cluster between Serbia, Slovakia and the Czech Republic. Multilateral cooperation will foster development of structural biology methods for study of pharmacologically active enzymes important for treatment of human cancers and pathogenic bacterial infections. Specifically we will merge expertise and resources in structural bioinformatics (Serbia), protein crystallization (Slovakia) and X-ray diffraction (Czech Republic) to accomplish our scientific goals at the study of endolysins, carbonic anhydrases and aldoketoreductases. Pharmacologically active enzymes affect the efficacy of clinical drugs by activation/inhibition of drug compounds, improving drug stability or delivery, or exposing new targets of action. By elucidating the molecular basis of these phenomena, this research will impact efforts to overcome drug resistance in human cancers and pathogenic infections.
Duration: 1.1.2017 - 31.12.2018

The total number of projects: 9