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Institute: Institute of Molecular Biology

Evolution of alpha-amylases and related enzymes
Evolúcia alfa-amyláz a príbuzných enzýmov
Program: Bilateral - other
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2015 - 31.12.2019

Protein design and evolution of raw starch digesting amylases
Proteínový dizajn a evolúcia amyláz degradujúcich surový škrob
Program: Bilateral - other
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2015 - 31.12.2019

Regulatory network of septin higher-order structures.
Regulačná sieť štruktúr vyššieho poriadku septínov.
Program: Bilateral - other
Project leader: RNDr. Farkašovský Marian CSc.
Annotation:Septins are conserved guanosine phosphate–binding proteins involved in cytokinesis and other essential membrane remodelling functions. They form heterooligomeric complexes, which polymerize into nonpolar filaments and are present in most eukaryotic organisms. Septin structures serve as a scaffold for the localized assembly of various proteins at the cell division plane. They also act as diffusion barrier for membrane-associated proteins, which generate different higher-order structures depending on the particular stage of the cell cycle. The mechanisms of formation, localization of septin structures and the regulation of septin ring assembly are difficult to study and therefore poorly understood. Our main goal is to explain how the protein kinases Elm1, Gin4, Cla4 and Cdc28 are involved in the regulation of coordinated assembly of septins in different stages of the cell cycle. We will study the in vitro phosphorylation of GFP-labelled septins by fluorescence microscopy observing the production of kinase reaction products in free solution and on supported lipid bilayer. Cryo electron microscopy and single particle analysis will be employed to obtain structural details of the phosphorylation-induced changes in septins. Observation of the localization and structural changes of the septin cortex in vivo as well as localization of interacting proteins at different conditions and in different genetic backgrounds (e.g. kinase mutants) will support our in vitro findings.
Duration: 1.1.2017 - 31.12.2019

Structure/function and evolution of amylolytic enzymes
Štruktúra, funkcia a evolúcia amylolytických enzýmov
Program: Bilateral - other
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2016 - 31.12.2020

Structure, function and evolution of trehalose synthases
Štruktúra, funkcia a evolúcia trehalózasyntáz
Program: Bilateral - other
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2015 - 31.12.2019

The total number of projects: 5