Specificity of the volume-activated amino acid efflux pathway in cultured human breast cancer cells

Rok, strany: 2011, 45 - 51

It has been shown that cell swelling stimulates the efflux of taurine from MCF-7 and MDA-MB-231 cells via a pathway which has channel-like properties. The purpose of this study was to examine the specificity of the volume-activated taurine efflux pathway in both cell lines. A hyposmotic shock increased the efflux of glycine, L-alanine, AIB (α-aminoisobutyric acid), D-aspartate but not L-leucine from MDA-MB-231 and MCF-7 cells. It was evident that the time course of activation/inactivation of those amino acids whose efflux was affected by cell swelling was similar to that of volume-activated taurine efflux. The effect of exogenous ATP on swelling-induced glycine, AIB and D-aspartate efflux from MDA-MB-231 cells was similar to that found on taurine efflux. In addition, volume-activated AIB efflux from MDA-MB-231 cells, like that of swelling-induced taurine efflux, was inhibited by diiodosalicylate. Tamoxifen inhibited volume-activated taurine release from both MDA-MB-231 and MCF-7 cells. The results suggest that neutral and anionic α-amino acids are able to utilize the volume-activated taurine efflux pathway in both cell lines. The effect of tamoxifen on breast cancer growth may, in part, be related to perturbations in cell volume regulation.

ISO 690:

Shennan, D., Thomson, J. 2011. Specificity of the volume-activated amino acid efflux pathway in cultured human breast cancer cells. In General Physiology and Biophysics, vol. 30, no.1, pp. 45-51. 0231-5882.

APA:

Shennan, D., Thomson, J. (2011). Specificity of the volume-activated amino acid efflux pathway in cultured human breast cancer cells. General Physiology and Biophysics, 30(1), 45-51. 0231-5882.