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Effect of normobaric oxygen treatment on oxidative stress and enzyme activities in guinea pig heart

In: General Physiology and Biophysics, vol. 31, no. 2
Zuzana Tatarkova - Ivan Engler - Andrea Calkovska - Daniela Mokrá - Anna Drgova - Stanislav Kuka - Peter Racay - Ján Lehotský - Dušan Dobrota - Peter Kaplan
Detaily:
Rok, strany: 2012, 179 - 184
O článku:
Normobaric oxygen (NBO) therapy is commonly applied for the treatment of various diseases, including myocardial infarctions, but its effectiveness is controversial. Potential adverse effects of hyperoxia are related to excessive formation of free radicals. In the present study we examined the effect of 60-h NBO treatment on lipid peroxidation (LPO), activity of manganese superoxide dismutase (Mn-SOD) and mitochondrial enzymes of energy metabolism in guinea pig heart. NBO treatment resulted in significant accumulation of thiobarbituric acid reactive substances and loss of Mn-SOD activity despite slight elevation of Mn-SOD protein content. Activity of electron transport chain complex III decreased significantly, while activity of complex IV was slightly elevated and citrate synthase was unchanged. LPO, inhibition of Mn-SOD and complex III activities were more pronounced when inhaled oxygen was partially enriched with superoxide radical. In contrast, when O2 was enriched with oxygen cation (O2●+), LPO and loss of Mn-SOD activity were prevented. Complex III activity in the O2●+-treated group remained depressed but activities of complex IV and citrate synthase were elevated. These data suggest that NBO treatment is associated with myocardial oxidative damage and attenuation of antioxidant defense, but these adverse effects can be partially attenuated by inhalation of O2 enriched with oxygen cation.
Ako citovať:
ISO 690:
Tatarkova, Z., Engler, I., Calkovska, A., Mokrá, D., Drgova, A., Kuka, S., Racay, P., Lehotský, J., Dobrota, D., Kaplan, P. 2012. Effect of normobaric oxygen treatment on oxidative stress and enzyme activities in guinea pig heart. In General Physiology and Biophysics, vol. 31, no.2, pp. 179-184. 0231-5882.

APA:
Tatarkova, Z., Engler, I., Calkovska, A., Mokrá, D., Drgova, A., Kuka, S., Racay, P., Lehotský, J., Dobrota, D., Kaplan, P. (2012). Effect of normobaric oxygen treatment on oxidative stress and enzyme activities in guinea pig heart. General Physiology and Biophysics, 31(2), 179-184. 0231-5882.