Dimethyl sulfoxide at high concentrations inhibits non-selective cation channels in human erythrocytes

Rok, strany: 2013, 23 - 32

Dimethyl sulfoxide (DMSO), a by-product of the pulping industry, is widely used in biological research, cryobiology and medicine. On cellular level DMSO was shown to suppress NMDA-AMPA channels activation, blocks Na+ channel activation and attenuates Ca2+ influx (Lu and Mattson 2001). In the present study we explored the whole-cell patch-clamp to examine the acute effect of high concentrations of DMSO (0.1–2 mol/l) on cation channels activity in human erythrocytes. Acute application of DMSO (0.1–2 mol/l) dissolved in Cl–-containing saline buffer solution significantly inhibited cation conductance in human erythrocytes. Inhibition was concentration-dependent and had an exponential decay profile. DMSO (2 mol/l) induced cation inhibition in Cl–- containing saline solutions of: 40.3 ± 3.9% for K+, 35.4 ± 3.1% for Ca2+ and 47.4 ± 1.9% for NMDG+. Substitution of Cl– with gluconate– increased the inhibitory effect of DMSO on the Na+ current. Inhibitory effect of DMSO was neither due to high permeability of erythrocytes to DMSO nor to an increased tonicity of the bath media since no effect was observed in 2 mol/l glycerol solution. In conclusion, we have shown that high concentrations of DMSO inhibit the non-selective cation channels in human erythrocytes and thus protect the cells against Na+ and Ca2+ overload. Possible mechanisms of DMSO effect on cation conductance are discussed.

ISO 690:

Nardid, O., Schetinskey, M., Kucherenko, Y. 2013. Dimethyl sulfoxide at high concentrations inhibits non-selective cation channels in human erythrocytes. In General Physiology and Biophysics, vol. 32, no.1, pp. 23-32. 0231-5882.

APA:

Nardid, O., Schetinskey, M., Kucherenko, Y. (2013). Dimethyl sulfoxide at high concentrations inhibits non-selective cation channels in human erythrocytes. General Physiology and Biophysics, 32(1), 23-32. 0231-5882.