IMPACT OF INHIBITORS OF HISTONE DEACETYLASES ON SURVIVAL AND INTRACELLULAR STRESS RESPONSES OF NEUROBLASTOMA SH-SY5Y CELLS.

Strany: 37 - 57

Jazyk: eng

Abstract Objectives: Histone deacetylases (HDAC) are enzymes that catalyse removal of acetyl group from lysine side chains of histones and non-histone proteins. Inhibitors of HDAC (HDACi) are extensively studied because of their ability to induce death of tumour cells. HDACi are also studied as neuroprotective molecules in different models of neurodegenerative diseases. We have examined impact of HDACi, butyrate and vorinostat, on relative survival of SH-SY5Y cells and intracellular stress response. Methods: Relative cell survival was determined by MTT assay and light microscopy. The expression of the key stress proteins was determined by Western blot analysis. Results: We have shown that both butyrate and vorinostat have significant negative impact on relative survival of SH-SY5Y cells. Treatment of the cells with vorinostat was associated with significantly increased expression of HRD1 that is protein of ER stress response. Expression of HRD1 was also elevated in the cells treated with butyrate but the increase was not statistically significant. Both butyrate and vorinostat did not significantly affect expression of mitochondrial HSP60 as a protein of mitochondrial stress and HSP70 that is upregulated after proteasome stress. Conclusion: Negative impact of vorinostat on survival of neuronal cells could account for neuropathy observed as an adverse effect of the treatment of cutaneous T-cell lymphoma with vorinostat. The potential of HDACi including vorinostat to induce ER stress proteins is probably not associated with neuproprotective effects of HDACi.

ISO 690:

Michalička, J., Lišková, M., Gužíková , J., Račaj, P. 2025. IMPACT OF INHIBITORS OF HISTONE DEACETYLASES ON SURVIVAL AND INTRACELLULAR STRESS RESPONSES OF NEUROBLASTOMA SH-SY5Y CELLS.. In Activitas Nervosa Superior Rediviva, vol. 67, no.2, pp. 37-57. 1337-933X. DOI: https://doi.org/10.31577/ansr.2025.67.2.2

APA:

Michalička, J., Lišková, M., Gužíková , J., Račaj, P. (2025). IMPACT OF INHIBITORS OF HISTONE DEACETYLASES ON SURVIVAL AND INTRACELLULAR STRESS RESPONSES OF NEUROBLASTOMA SH-SY5Y CELLS.. Activitas Nervosa Superior Rediviva, 67(2), 37-57. 1337-933X. DOI: https://doi.org/10.31577/ansr.2025.67.2.2

Vydavateľ: Centrum Experimentálnej Medicíny SAV, v. v. i.