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DNA–DOPE–gemini surfactants complexes at low surface charge density: from structure to transfection efficiency

In: General Physiology and Biophysics, vol. 37, no. 1
Lukáš Hubčík - Dominika Galliková - Petra Pullmannová - Ľubica Lacinová - Zdena Sulová - Mária Hanulová - Sergio Funari - Ferdinand Devínsky - Daniela Uhrikova
Detaily:
Rok, strany: 2018, 57 - 69
O článku:
DNA condensation, structure and transfection efficiency of complexes formed by gemini surfactants alkane-α,ω-diyl-bis(dodecyldimethylammonium bromide)s (CnGS12, n = 3, 6 and 12 is the number of alkane spacer carbons), dioleoylphosphatidylethanolamine (CnGS12/DOPE = 0.3 mol/mol) and DNA at low surface charge density were investigated through different techniques. Small angle X-ray diffraction showed a condensed lamellar phase with marked dependence of DNA-DNA distance on (+/-) charge ratio. High ionic strength of hydrating medium screens the interaction DNA - CnGS12/DOPE and complexed DNA represented maximally ~ 45–60% of total DNA in the solution as derived from fluorescence and UV-VIS spectroscopy. The in vitro transfection efficiency of CnGS12/DOPE liposomes on mammalian HEK 293 cell line was spacer length-dependent. C12GS12/DOPE/DNA complexes exhibited the best transfection efficiency (~ 18% GFP-expressing cells relative to all viable cells) accompanied by ~ 89% cell viability.
Ako citovať:
ISO 690:
Hubčík, L., Galliková, D., Pullmannová, P., Lacinová, Ľ., Sulová, Z., Hanulová, M., Funari, S., Devínsky, F., Uhrikova, D. 2018. DNA–DOPE–gemini surfactants complexes at low surface charge density: from structure to transfection efficiency. In General Physiology and Biophysics, vol. 37, no.1, pp. 57-69. 0231-5882.

APA:
Hubčík, L., Galliková, D., Pullmannová, P., Lacinová, Ľ., Sulová, Z., Hanulová, M., Funari, S., Devínsky, F., Uhrikova, D. (2018). DNA–DOPE–gemini surfactants complexes at low surface charge density: from structure to transfection efficiency. General Physiology and Biophysics, 37(1), 57-69. 0231-5882.