Mitochondrial hyperpolarization and cytochrome-c release in microwave-exposed MCF-7 cells

Rok, strany: 2017, 211 - 218

This study examines the effects of a 2.1-GHz WCDMA-modulated microwave (MW) radiation on apoptotic activity and mitochondrial membrane potential (ΔΨm) in MCF-7 cells. The cells were exposed to the MW at a specific absorption rate (SAR) of 0.528 W/kg for 4 or 24 h. The antiproliferative effect of MW exposure was determined by the MTT test. Cytochrome-c and p53 levels were determined by an ELISA method. The relative ΔΨm was analysed by JC-1 staining using flow cytometer. Apoptotic rate of the cells was measured by Annexin-V-FITC staining. All assays were performed after certain time of incubations (15 min–4 h) following MW exposure. MW-exposed cells showed a significant decrease in viability when compared to unexposed cells. A significantly larger decrease was observed after longer exposure. The percentage of apoptotic cells, amount of cytochrome-c, and relative ΔΨm were significantly higher in MW-exposed cells. The percent of apoptotic cells and relative ΔΨm in 24 h MW-exposed group was significantly higher than those in 4 h MW-exposed group. However, no significant change was observed in p53 levels. These results demonstrated that exposure to 2.1-GHz WCDMA-modulated MW radiation caused hyperpolarization of mitochondria that in turn induced apoptosis in MCF-7 cells.

ISO 690:

Esmekaya, M., Canseven, A., Kayhan, H., Tuysuz , M., Sirav, B., Seyhan, N. 2017. Mitochondrial hyperpolarization and cytochrome-c release in microwave-exposed MCF-7 cells. In General Physiology and Biophysics, vol. 36, no.2, pp. 211-218. 0231-5882.

APA:

Esmekaya, M., Canseven, A., Kayhan, H., Tuysuz , M., Sirav, B., Seyhan, N. (2017). Mitochondrial hyperpolarization and cytochrome-c release in microwave-exposed MCF-7 cells. General Physiology and Biophysics, 36(2), 211-218. 0231-5882.