Centre of Experimental Medicine SAS
Evaluation of effects of natural and synthetic compounds in vitro and in vivo
Name of the supervisor
RNDr. Katarína Valachová , PhD.
Faculty of Natural Sciences Comenius University
Hyaluronan (HA), a polysaccharide of molecular weight of several megaDaltons, is present in all tissues of vertebrates. In inflammatory diseases the average molecular weight of hyaluronan is significantly decreased by action of reactive oxygen species. The solutions of hyaluronan of high molecular weight will be exposed to free radicals and oxidants in in vitro studies. Further, protective effects of antioxidants/drugs to prevent and chain-break degradation of HA will be examined. The compounds of synthetic origin such as antiinflammatory drugs, e.g. mitochondria-targeted antioxidants and natural compounds, which will function as antioxidants against in situ degradation of HA will be examined against a) oxidative demage of cell lines and b) in vivo in skin wounds of ischemic rabbits.