Centre of Experimental Medicine SAS
Topic
In vitro and in vivo evaluation of medicinal effects of natural and synthetic compounds for the treatment of skin wounds by composite membranes
PhD. program
Biochemistry
Year of admission
2025
Name of the supervisor
RNDr. Katarína Valachová , PhD.
Contact:
Receiving school
Faculty of Chemical and Food Technology STU
Annotation
Hyaluronan (HA) is a high-molar-weight polysaccharide (up to 7 MDa) occurring especially in the skin, vitreous body, synovial fluid and umbilical cord. In inflammatory diseases the molar mass of HA is decreased by oxidants and reactive oxygen species.
In vitro the solutions of high-molar-mass will be exposed to free-radical degradation by using rotational viscometry as well as oximetric monitoring of oxygen consumption. i) Preventive and ii) chain-breaking effects of natural and synthetic compounds will be explored in prevention of HA degradation. Compounds with significant i) and ii) effects will be subsequently assessed by in vivo experiments of the treatment of skin wounds in ischemic rabbits, healthy and diabetic rats.
In vitro the solutions of high-molar-mass will be exposed to free-radical degradation by using rotational viscometry as well as oximetric monitoring of oxygen consumption. i) Preventive and ii) chain-breaking effects of natural and synthetic compounds will be explored in prevention of HA degradation. Compounds with significant i) and ii) effects will be subsequently assessed by in vivo experiments of the treatment of skin wounds in ischemic rabbits, healthy and diabetic rats.