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PhD. Topics

Centre of Biosciences SAS

Bacteria from the digestive tract of bees with a probiotic and bacteriogenic potential
PhD. program
Animal physiology
Name of the supervisor
RNDr. Anna Kopčáková, PhD.
Receiving school
Faculty of Science, Pavol Jozef Šafárik University in Kosice
In last few years bee significant deterioration of bee health status is observed trough the world. American Foulbrood is the most devastating bacterial disease affecting honeybee brood worldwide. The causes this disease is Gram-positive the spore-forming bacterium of Paenibacillus larvae. At the present time there is no treatment available for American foulbroad disease. Globally, the bee´s mortality is addressed radically, but in some countries antibiotics are allowed to be used to treat this disease, with the result that antibiotic residues are obtained in honey. At the moment the potential treatment method is use application of biological methods based on probiotics and other natural substances like bacteriocins. The aim of the PhD thesis will be to obtain comprehensive information on the composition of microbiome digestive tract of bees using classical cultivation methods combined with non-cultivating metagenomic approach. When analyzing the genetic diversity of bee digestive microbiome, we will be use methods based on the small subunit of bacterial rRNA gene (16S PCR DGGE and metagenomic analysis). In cultivation methods, isolates obtained will be identified by classical microbiological techniques as well as modern proteomic and genetic molecular methods (MALDI TOF, 16S rRNA sequencing, whole gene sequencing). In selected isolates from the bee´s digestive tracts we will study their probiotic and bacteriocinogenic potential.