Electronic Library of Scientific Literature - © Academic Electronic Press
Original articles Macho L, Zorad S, Radikova Z, Patterson-Buckedahl P,
Kvetnansky R (Bratislava, Slovakia and Pistacaway, NJ, USA) Medvedova L, Knopp J, Farkas R (Bratislava, Slovakia) Ostrowska Z, Kos-Kudla B, Nowak M, Swietochowska E, Marek
B, Gorski J, Kajdaniuk D, Wolkowska K (Zabrze, Poland) Review Stepan JJ, Alenfeld F, Boivin G, Feyen JHM, Lakatos P
(Praha, Czech Republic; Berlin, Germany; Lyon, France; Princeton, NJ and
Budapest, Hungary) Author Index, Vol. 37 Subject Index, Vol. 37
Volume 37 / No. 4 / 2003
Ethanol consumption affects stress response and insulin binding
in tissues of rats
Steroid regulation of terminal protein glycosyltransferase
genes: molecular and functional homologies within sialyltransferase and
fucosyltransferase families
The relationship between bone metabolism, melatonin and other
hormones in sham-operated and pinealectomized rats
Mechanisms of action of antiresorptive therapies of
postmenopausal osteoporosis
Endocrine Regulations (since 1967 to 1990 Endocrinologia Experimentalis) is an international journal on experimental and clinical endocrinology edited quarterly in English by care of the Institute of Experimental Endocrinology, Slovak Academy of Sciences (Bratislava, Slovakia) and published by the Slovak Academic Press (Bratislava, Slovakia).
This journal aims to publish original manuscripts or minireviews on experimental and clinical endocrinology and diabetes.
The submission of a manuscript to Endocrine Regulations implies that it has not been previously published or is not being submitted for publication elsewhere and that the manuscript has been approved by all authors who are ready to take public responsibility for the content.
All materials relating to human investigation will be published upon the understanding that design of the work has been approved by the local Ethical Committee or that it conforms to ethical guidelines of the Declaration of Helsinki. The animal experiments should state the conformance to guidelines on animal care.
Manuscripts in triplicate with three sets of illustrations (of which one is an original) should be sent to:
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Institute of Experimental Endocrinology,
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Itoh M, Okugawa T, Shiratori N, Ohashi H: Treatment with triiodothyronine (T3) against multinodular goiter fails to prevent the onset of Graves disease. Endocrine Regul 29, 151-156, 1995
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Mornex R, Orgiazzi JJ: Hyperthyroidism. In: The Thyroid Gland (Ed. M de Visscher), pp. 279-362, Raven Press, New York 1980
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Podoba J: Endemic goiter in Slovakia. VEDA, Bratislava, 1962
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Ladislav Macho, Stefan Zorad, Zofia Radikova, 1Patricia Patterson-Buckedahl, Richard Kvetnansky
Institute of Experimental Endocrinology, Slovak Academy of
Sciences, 833 06 Bratislava, Slovakia;
1Center of Alcohol Studies, Rutgers University, Pistacaway, N.J., USA,
e-mail: ueenlaco@savba.sk
Objective. The mechanisms of harmful effect of
excessive chronic ethanol intake on cardiovascular and neuronal systems,
metabolic processes and protective effects of low ethanol intake on
cerebrovascular and cardiovascular disturbances are not yet known. The aim of
present study was to investigate the effect of short-term and long-term ethanol
consumption on food intake, plasma levels of corticosterone (CS), glucose (G)
and insulin (INS) in intact rats and in animals exposed to immobilization stress
(IMO) or restrain stress (RESTR). The insulin binding to specific membrane
receptors in adipocytes, muscle and liver was also determined.
Methods. Adult male rats were fed liquid diet without and with ethanol (5 % per
weight) for 9-12 days (short-term intake) and at the end the animals were
exposed to acute IMO stress. The second group of rats was fed solid diet and
without or with ethanol in drinking water (6 % per volume) for 52 days
(long-term ethanol intake). A part of these animals was exposed to repeated
restrain stress during 42 days. The groups of pair fed rats with the same food
intake as in ethanol diet fed animals were in both experiments. The food intake,
plasma glucose, insulin and corticosterone levels were determined. Plasma cell
membranes were isolated from adipose, liver and skeletal muscle tissues and
insulin binding to specific receptors was determined.
Results. Decreased food consumption was observed after ethanol intake.
Increased plasma G and CS were noted in rats fed ethanol diet for a short
time. Plasma insulin levels were not affected by ethanol intake. Exposure to IMO
stress resulted in increase of plasma G levels in controls and pair fed groups.
A higher increase of CS plasma levels after IMO stress was noted in rats
with ethanol intake for a short-time, however, no changes of plasma CS were
noted after repeated exposure to restrain stress. Plasma levels of insulin were
decreased after IMO and restrain stresses, while in rats fed ethanol diet for
a short-time insulin decrease was deeper as compared to controls. The
binding capacity of insulin receptors in adipose tissue was elevated in rats fed
ethanol diet and deep decrease of insulin binding was noted in rats exposed to
stress. In liver insulin binding was elevated after short-term ethanol intake
and stress exposure resulted in decrease of insulin binding in ethanol fed rats.
The binding capacity of insulin receptors in skeletal muscle was not changed in
rats fed ethanol diet.
Conclusions. The results showed 1. differences in short term and long term
ethanol intake on basal glucose, insulin and corticosterone levels, 2. effects
of ethanol intake on changes of insulin plasma levels and insulin binding in
adipose and liver tissues after exposure to stress, 3. effects of short term
ethanol intake on the response of plasma hormones to immobilization stress.
Key words: Ethanol intake - Stress response - Glycemia - Insulinemia -
Insulin biding - Rats
ENDOCRINE REGULATIONS, Vol. 37, 195–202, 2003
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Lucia Medvedova1, Jan Knopp , Robert Farkas
Institute of Experimental Endocrinology, Slovak Academy of
Sciences, 833 06 Bratislava, Slovakia;
1Department of Genetics, Graduate Programme, Faculty of Science,
Comenius University, Bratislava, Slovakia
Objective. Terminal glycosylations such as
fucosylation and especially sialylation are crucial in maturation of proteins
for their particular function. In cells and tissues where their function is
under hormonal control, often terminal processes including glycosylations are
tightly regulated by hormones. Therefore, it is not surprising that two key
enzyme families involved in these steps, fucosyltransferases and
sialyltransferases, are directly controlled by steroid hormones.
Methods. Gene structure and protein sequences have been analyzed by set of
Unix-based complex sequence analysis programs of the Wisconsin GCG package, and
from some other resources. Protein secondary structure predictions were based on
several independent programs and applications complementing each other depending
on the algorithm used, all running on Unix platforms.
Results. Comparison of these two types of enzymes from organisms where
they are known to be under direct hormonal regulation has revealed that although
sialyltransferases are broad family of proteins, the only known enzymes to be
under hormonal control are a-2,6-sialyltransferases and in the case of fucosyl
moieties transferring enzymes these are a-1,2/6-fucosyltransferases.
Interestingly, hormonally regulated sialyltransferases from as evolutionary
distant organisms as human and fruitfly share several important features
including secondary structure characteristics encompassing sialyl L and S motifs
in which they differ from other sialyltransferases. Analogous organizational and
structural similarities were found also in hormonally regulated
fucosyltransferases.
Conclusions. Data indicate that hormonal control of these classes of
enzymes as a regulatory mechanism could be attained relatively early during the
evolution in order to obtain selective control over specific protein
modification process which plays an important role during ontogenic development.
Key words: Steroid hormones - Sialyltransferase - Fucosyltransferase -
Gene structure and expression - Protein sequence and secondary structure
ENDOCRINE REGULATIONS, Vol. 37, 203–210, 2003
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Z. Ostrowska, B. Kos-Kudla1, M. Nowak1, E. Swietochowska, B. Marek1, J. Gorski, D. Kajdaniuk1, K. Wolkowska
Department of Clinical Biochemistry,
1Department of Pathophysiology and Endocrinology, The Medical
University of Silesia, Zabrze, Poland
E-mail: ozdrasiek@poczta.onet.pl
Objective. The influence of pinealectomy and long-term
melatonin (MEL) administration on circadian oscillations of selected biochemical
markers of bone metabolism [serum alkaline phosphatase (ALP) activity,
carboxyterminal propeptide type I procollagen (PICP) and carboxyterminal
telopeptide type I collagen (ICTP) concentrations as well as urinary excretion
of hydroxyproline (HYP) and Ca] and possible involvement of circadian secretion
of IGF-I, parathyroid, thyroid, adrenal cortex and gonads function in this
mechanism was evaluated.
Methods. Studies were performed in 192 adult male Wistar rats weighing 145±9 g
which were subjected to pinealectomy or sham operation. In half of the animals
from each group MEL (Sigma, USA) in a dose of 50 mg/100 g b.w. was
administered intraperitonealy (daily between 17.00 and 18.00 h for a 4-week
period). Material for studies (blood and urine) was collected every 3 hours
during a day. Hormones, PICP and ICTP concentrations were determined with
the use of RIA methods, whereas ALP, HYP and Ca values - spectrophotometrically.
Results. The study has shown that pinealectomy had an inducing, while
exogenous MEL a suppressing effect upon the level of investigated
biochemical markers of bone metabolism. Furthermore, substantial changes in the
values of amplitude and phase of their circadian oscillations were shown.
Distinct, dependent on the time of day disturbances in circadian fluctuations of
PICP, ICTP, HYP and Ca showing generally negative correlation with changes in
endogenous MEL concentrations and positive with IGF-I and corticosterone (B)
levels were found. In addition, changes in circadian oscillations of ALP and
PICP correlated negatively with daily oscillations of calciotropic hormones and
B. However, ICTP, HYP and Ca concentrations correlated positively with circadian
fluctuations of B and FT3 (the latter only in sham operated rats receiving MEL).
Conclusions. This study showed that both pinealectomy and long-term MEL
administration influence the circadian rhythm of bone metabolism and that an
important role in the mechanism of this dependence is played by the changes of
endogenous MEL levels. Secondary changes in circadian oscillations of
calciotropic hormones, IGF-I and corticosterone concentrations, caused by
pinealectomy and long-term MEL administration result in altered bone metabolism
rhythm.
Key words: Pinealectomy - Melatonin - Bone metabolism - Hormones -
Circadian rhythm - Male rats
ENDOCRINE REGULATIONS, Vol. 37, 211–224, 2003
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J. J. Stepan, 1F. Alenfeld, 2G. Boivin, 3J. H. M. Feyen, 4P. Lakatos
Charles University School of Medicine, Prague, Czech
Republic,
1 Zentrum für Muskel- und Knochenforschung, Klinikum Benjamin
Franklin, Berlin, B.R.D
2 INSERM Unité 403, Faculté de Médecine R. Laennec, 69372
Lyon Cedex 08, France,
3 Bristol-Myers Squibb, Princeton NJ, USA,
41st Department of Medicine, Semmelweis University Medical
School, Budapest, Hungary
E-mail: jstepan@vol.cz
In the treatment of osteoporosis, the aim of the
antiresorptive therapy is to restore bone density by decreasing bone remodeling.
The process of bone remodeling plays a role in plasma calcium homeostasis
and serves to modify bone architecture in order to meet changing mechanical
needs, to maintain osteocyte viability, and to repair microdamage in bone
matrix. Estrogen deficiency results in a number of detrimental effects on
bone, including suppression of osteocyte survival as well as impairment of
osteoblast response to mechanical stimuli and repair of ageing bone. In this
review, effects of available antiresorptive therapies on endocrine regulations
of bone metabolism in postmenopausal osteoporosis are compared.
The aim of antiresorptive treatment is to ensure adequate bone remodeling,
reparation of microdamage of bone, and increased bone strength. Ideally, this
effect should be maintained long-term. Several agents are approved for the
treatment of osteoporosis. Calcitonin transiently inhibits osteoclast activity
without decreasing osteoblast collagen synthesis. Aminobisphosphonates decrease
bone remodeling by decreasing osteoclast activity and by inducing osteoclast
apoptosis. This allows more time for secondary mineralization to proceed to
completion in the existing bone tissue mass, so increasing the mechanical
resistance of bone to loading. Estrogens and raloxifene (a selective estrogen
receptor modulator that acts as an estrogen agonist in bone) suppress bone
remodeling to the premenopausal range, maintaining the function of osteoblasts
and osteocytes. In the placebo-controlled osteoporosis treatment trials, all the
above treatments reduced the risk of fractures. Raloxifene therapy was also
associated with a favorable or neutral effect in the cardiovascular system,
and a reduced incidence of breast cancer. Selection of appropriate drug for
treatment of postmenopausal osteoporosis should take into account the long-term
effect of the antiresorptive agent on bone. Moreover, the effects on other
tissues ++should also be considered, and this encompasses both safety concerns,
as well as the potentially beneficial effects on other tissues. Further
investigation is needed to evaluate the different modes of action of these
agents, and their long-term effects on bone and other tissues.
Key Words: Apoptosis - Bisphosphonates - Calcitonin - Cytokines -
Estrogen - Osteoblasts -Osteoclasts - SERM - Raloxifene
ENDOCRINE REGULATIONS, Vol. 37, 227–240, 2003
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