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Effect of Pb2+ ions on photosynthetic apparatus

In: General Physiology and Biophysics, vol. 33, no. 1
Frantisek Sersen - Katarina Kralova - Matus Pesko - Marek Cigan
Detaily:
Rok, strany: 2014, 131 - 136
O článku:
Using model lead compounds Pb(NO3)2 and Pb(CH3CHOO)2, the mechanism and the site of action of Pb2+ ions in the photosynthetic apparatus of spinach chloroplasts were studied. Both compounds inhibited photosynthetic electron transport (PET) through photosystem 1 (PS1) and photosystem 2 (PS2), while Pb(NO3)2 was found to be more effective PET inhibitor. Using EPR spectroscopy the following sites of Pb2+ action in the photosynthetic apparatus were determined: the water-splitting complex and the Z•/D• intermediates on the donor side of PS2 and probably also the ferredoxin on the acceptor side of PS1, because cyclic electron flow in chloroplasts was impaired by treatment with Pb2+ ions. Study of chlorophyll fluorescence in suspension of spinach chloroplasts in the presence of Pb2+ ions confirmed their site of action in PS2. Using fluorescence spectroscopy also formation of complexes between Pb2+ and amino acid residues in photosynthetic proteins was confirmed and constants of complex formation among Pb2+ and aromatic amino acids were calculated for both studied lead compounds.
Ako citovať:
ISO 690:
Sersen, F., Kralova, K., Pesko, M., Cigan, M. 2014. Effect of Pb2+ ions on photosynthetic apparatus. In General Physiology and Biophysics, vol. 33, no.1, pp. 131-136. 0231-5882.

APA:
Sersen, F., Kralova, K., Pesko, M., Cigan, M. (2014). Effect of Pb2+ ions on photosynthetic apparatus. General Physiology and Biophysics, 33(1), 131-136. 0231-5882.