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Connexin 36 is expressed and associated with zonula occludens-1 protein in PC-12 cells

In: General Physiology and Biophysics, vol. 26, no. 1
S. Lu - H. Li - F. Zhou - J. Zhang - L. Wang
Detaily:
Rok, strany: 2007, 33 - 39
O článku:
Connexin 36 (Cx36) is the predominant connexin isoform expressed in the mammalian neurons of the central nervous system (CNS). PC-12 cells, a neuronal-like cell line, are widely used for neuron functional studies. Many connexins have been shown to interact with zonula oc-cludens-1 protein (ZO-1), a tight junction associated with protein. The present study is intended to investigate whether Cx36 is expressed in PC-12 cells and is associated with ZO-1. Cx36 transcripts were amplified and verified by RT-PCR. 2.9 kb Cx36 mRNA was detected in PC-12 cells through Northern blot hybridization. Western blotting showed a 36-kDa protein band in the homogenates of PC-12 cells. Immunofluorescence labeling revealed that Cx36 was present in cell-cell contacts of PC-12 cells and colocalized with ZO-1. The association of Cx36 and ZO-1 in PC-12 cells was also demonstrated by coimmunoprecipitation. In conclusion, PC-12 cells express Cx36 mRNA and Cx36 proteins that are associated with ZO-1. These results enhanced our understanding of the function of Cx36 in PC-12 cells.
Ako citovať:
ISO 690:
Lu, S., Li, H., Zhou, F., Zhang, J., Wang, L. 2007. Connexin 36 is expressed and associated with zonula occludens-1 protein in PC-12 cells. In General Physiology and Biophysics, vol. 26, no.1, pp. 33-39. 0231-5882.

APA:
Lu, S., Li, H., Zhou, F., Zhang, J., Wang, L. (2007). Connexin 36 is expressed and associated with zonula occludens-1 protein in PC-12 cells. General Physiology and Biophysics, 26(1), 33-39. 0231-5882.