Effects of β-hydroxybutyric acid on bovine milk leukocytes function in vitro

Rok, strany: 2007, 14 - 19

The in vitro effect of different concentrations of β-hydroxybutyric acid (βHBA) on bovine milk leukocytes was examined. βHBA level similar to those found in cows with clinical ketosis in-duced a significant inhibitory effect on the nitroblue tetrazolium reduction as a mean of assaying the metabolic integrity of macrophages after the phorbol-mirystate- acetate and opsonized zymosan stimulation. In the same way, the H2O2 production after stimulation with both soluble and par-ticulate agents decreased significantly in 33 and 26%, respectively, compared with cells incubated without ketone bodies. This result suggests a possible fault in the microbicidal oxidative activity. The macrophage phagocytosis also decreased in cells treated with different βHBA concentrations, in relation to that obtained from control cells. Neutrophils migration in agarose was determined, and the mean chemotactic response was higher when the cells were incubated with lower level or absence of ketone bodies. Considering the determined differences, we hypothesize that abnormally high levels of ketone bodies could produce a direct effect on leukocyte membranes. The induction of some modification on the receptor structure impairment the interaction ligand-receptor and this may be, in part, responsible for the higher susceptibility to local infections in mammary gland during subclinical and clinical ketosis.

ISO 690:

Cerone, S., Sansinanea, A., García, M. 2007. Effects of β-hydroxybutyric acid on bovine milk leukocytes function in vitro. In General Physiology and Biophysics, vol. 26, no.1, pp. 14-19. 0231-5882.

APA:

Cerone, S., Sansinanea, A., García, M. (2007). Effects of β-hydroxybutyric acid on bovine milk leukocytes function in vitro. General Physiology and Biophysics, 26(1), 14-19. 0231-5882.