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Characterization and regulation of basal calcium influx in human peripheral blood lymphocytes

In: General Physiology and Biophysics, vol. 27, no. 4
Boris Lakatoš - Jana Slováková - Karin Kaiserová - Jozef Orlický - Ľudovít Varečka
Detaily:
Rok, strany: 2008, 253 - 262
O článku:
The basal 45Ca2+ influx into resting human blood lymphocytes was measured. This process showed biphasic kinetics with first rapid phase followed by the second long-lasting and markedly slower phase. Further, it showed signs of saturability and reaches maximal values at 37°C and extracellular pH 7.2. The basal 45Ca2+ influx was stimulated by addition of submicromolar concentrations of 4β-phorbol-12-myristate-13-acetate, and this effect was abolished by protein kinase C (PKC) inhibitor Ro-31-8220. In the regulation of basal 45Ca2+ influx is probably only partially involved adenylate cyclase pathway as show results with intracellular c-AMP elevating agents (dB-c-AMP, 3-isobutyl-1-metylxantine and forskolin). Uncoupler 3,3',4',5-tetrachloro-salicylanilide (TCS) in micromolar concentrations stimulated basal 45Ca2+ influx and its effect was more significant in media with high extracellular concentration of K+.
Ako citovať:
ISO 690:
Lakatoš, B., Slováková, J., Kaiserová, K., Orlický, J., Varečka, Ľ. 2008. Characterization and regulation of basal calcium influx in human peripheral blood lymphocytes. In General Physiology and Biophysics, vol. 27, no.4, pp. 253-262. 0231-5882.

APA:
Lakatoš, B., Slováková, J., Kaiserová, K., Orlický, J., Varečka, Ľ. (2008). Characterization and regulation of basal calcium influx in human peripheral blood lymphocytes. General Physiology and Biophysics, 27(4), 253-262. 0231-5882.