In: General Physiology and Biophysics, vol. 28, no. 3
Danijela Krstić - Mirjana Čolović - Nada Bošnjaković-Pavlović - Anne Spasojević-De Bire - Vesna Vasić
Detaily:
Rok, strany: 2009, 302 - 308
O článku:
The in vitro influence of decameric vanadate species on Na+/K+-ATPase, plasma membrane Ca2+-ATPase (PMCA)-calcium pump and ecto-ATPase activity, using rat synaptic plasma membrane (SPM) as model system was investigated, whereas the commercial porcine cerebral cortex Na+/K+-ATPase served as a reference. The thermal behaviour of the synthesized decavanadate (V10 ) has been studied by differential scanning calorimetry and thermogravimetric analysis, while the type of polyvanadate anion was identified using the IR spectroscopy. The concentration-dependent responses to V10 of all enzymes were obtained. The half-maximum inhibitory concentration (IC50 ) of the enzyme activity was achieved at (4.74 ± 1.15) × 10−7 mol/l for SPM Na+/K+-ATPase, (1.30 ± 0.10) × 10−6 mol/l for commercial Na+/K+-ATPase and (3.13 ± 1.70) × 10−8 mol/l for Ca2+-ATPase, while ecto-ATPase is significantly less sensitive toward V10 (IC50 = (1.05 ± 0.10) × 10−4 mol/l) than investigated P-type ATPases. Kinetic analysis showed that V10 inhibited Na+/K+-ATPase by reducing the maximum enzymatic velocity and apparent affinity for ATP (increasing Km value), implying a mixed mode of interaction between V10 and P-type ATPases.
Ako citovať:
ISO 690:
Krstić, D., Čolović, M., Bošnjaković-Pavlović, N., Spasojević-De Bire, A., Vasić, V. 2009. Influence of decavanadate on rat synaptic plasma membrane ATPases activity. In General Physiology and Biophysics, vol. 28, no.3, pp. 302-308. 0231-5882.
APA:
Krstić, D., Čolović, M., Bošnjaković-Pavlović, N., Spasojević-De Bire, A., Vasić, V. (2009). Influence of decavanadate on rat synaptic plasma membrane ATPases activity. General Physiology and Biophysics, 28(3), 302-308. 0231-5882.