In: General Physiology and Biophysics, vol. 28, no. 3
Bohumila Tarabová - Marie Novakova - Ľubica Lacinová
Detaily:
Rok, strany: 2009, 249 - 259
O článku:
Effects of haloperidol on L-type CaV 1.2 channel were studied. Calcium current was measured in whole cell patch-clamp using calcium as a charge carrier. Inhibition by haloperidol was investigated in CaV 1.2 channel natively expressed in rat cardiac myocytes and recombinant cardiac (CaV 1.2a ) and vascular (CaV 1.2b ) splice variants of the channel expressed in HEK 293 cells. Haloperidol inhibited L-type calcium current in a concentration-dependent manner with a threshold of 1 nmol/l. 1 µmol/l haloperidol inhibited 20.6 ± 3.6% of calcium current amplitude in cardiomyocytes, 25.4 ± 2.6% of current amplitude through the CaV 1.2b channel and 28.0 ± 2.7% of current through the CaV 1.2a channel. Inhibition was not accompanied by alteration of current waveform or by shift of current-voltage relation. In a current clamp haloperidol suppressed action potential generation. 1 µmol/l of the drug shortened the action potential duration in part of the cells and suppressed fully action potential in other cells. Moderate inhibition of the L-type calcium channels by haloperidol might cause shortening of action potential. Complete abolishment of action potential must have been mediated by inhibition of another, likely sodium channel.
Ako citovať:
ISO 690:
Tarabová, B., Novakova, M., Lacinová, Ľ. 2009. Haloperidol moderately inhibits cardiovascular L-type calcium current. In General Physiology and Biophysics, vol. 28, no.3, pp. 249-259. 0231-5882.
APA:
Tarabová, B., Novakova, M., Lacinová, Ľ. (2009). Haloperidol moderately inhibits cardiovascular L-type calcium current. General Physiology and Biophysics, 28(3), 249-259. 0231-5882.