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Exposure to fractionated dose of 60 Gy affects molecular response of HL‐60 cells to irradiation

In: General Physiology and Biophysics, vol. 29, no. 3
Jiřina Vávrová - Aleš Tichý - Martina Řezáčová - Lenka Zárybnická - Zuzana Šinkorová - Jan Österreicher
Detaily:
Rok, strany: 2010, 275 - 281
O článku:
In this work we evaluated changes in molecular response of human promyelocyte leukemia cells HL‐60 and HL‐60‐IR cells (HL‐60 irradiated by 10 cycles of radiation with total dose of 60 Gy, given over a period of 3 months) to irradiation by the dose of 2 and 8 Gy. Analysis of CD11b and apoptosis by flow‐cytometry revealed that on 3rd day after irradiation by 8 Gy the HL‐60‐IR are more resistant to radiation‐induced apoptosis and more differentiated (increase in CD11b in non‐apoptotic cells) than regular HL‐60. We found that both types of cells have high basal level of phosphorylated extracellular signal‐regulated kinases Erk1/2 . Irradiation induces decrease in Erk1/2 phosphorylation after 4 and 8 h in both cell types. However, in HL‐60‐IR cells Erk1/2 phosphorylation is restored faster than in HL‐60. Also it was found that in contrary to HL‐60 cells, the HL‐60‐IR cells react to 2 Gy irradiation by p53 independent increase in p21(WAF1/Cip1), and not by activation of checkpoint kinase Chk‐2. Therefore we concluded that relatively high dose of radiation (6 Gy) does not lead after 10 repetitive irradiations to eradication of HL‐60 cells, but instead increases their radioresistance, increases the ability to differentiate, alters MAPK response, increases amount of p21(WAF1/Cip1), and decreases induction of apoptosis by ionizing radiation. p21(WAF1/Cip1) might prevent apoptosis induction and trigger permanent cell‐cycle arrest, which can also contribute to regression of this leukaemia after therapy.
Ako citovať:
ISO 690:
Vávrová, J., Tichý, A., Řezáčová, M., Zárybnická, L., Šinkorová, Z., Österreicher, J. 2010. Exposure to fractionated dose of 60 Gy affects molecular response of HL‐60 cells to irradiation. In General Physiology and Biophysics, vol. 29, no.3, pp. 275-281. 0231-5882.

APA:
Vávrová, J., Tichý, A., Řezáčová, M., Zárybnická, L., Šinkorová, Z., Österreicher, J. (2010). Exposure to fractionated dose of 60 Gy affects molecular response of HL‐60 cells to irradiation. General Physiology and Biophysics, 29(3), 275-281. 0231-5882.