Influence of sub‐chronic diabetes mellitus on functional properties of renal Na⁺,K⁺‐ATPase in both genders of rats

Rok, strany: 2010, 266 - 274

For characterization of Na⁺,K⁺‐ATPase, a key enzyme involved in maintenance of intracellular sodium homeostasis, expression of α1 subunit and the ATP‐ and Na⁺‐binding properties were investigated by Western blot analysis and by enzyme kinetics, respectively. Previous studies documented time‐dependent alteration of properties of renal Na⁺,K⁺‐ATPase from its mobilization after 8 days to serious deteriorations after 16 weeks of diabetes in rats. Characterizing the critical period during development of the disease, when mobilization of Na⁺,K⁺‐ATPase observed in the acute phase turns to its damage, we examined the enzyme properties after 8 weeks lasting diabetes which was induced by a single intraperitoneal administration of streptozotocin in a dose of 65 mg·kg⁻¹. The unchanged expression of Na⁺,K⁺‐ATPase α1‐subunit in both genders indicates that 8 weeks represent the time when the mobilization of enzyme synthesis observed previously in acute diabetes is lost. In this time the renal Na⁺,K⁺‐ATPase undergoes structural changes in the vicinity of Na⁺‐binding site resulting in worsened affinity to sodium in both genders as indicated by 13% and 18% increase of KNa value in female and male rats, respectively. However, gender specific was the diabetes‐induced decrease in affinity to ATP by 18% which occurred in female rats only.

ISO 690:

Javorková, V., Mézešová, L., Vlkovičová, J., Vrbjar, N. 2010. Influence of sub‐chronic diabetes mellitus on functional properties of renal Na⁺,K⁺‐ATPase in both genders of rats. In General Physiology and Biophysics, vol. 29, no.3, pp. 266-274. 0231-5882.

APA:

Javorková, V., Mézešová, L., Vlkovičová, J., Vrbjar, N. (2010). Influence of sub‐chronic diabetes mellitus on functional properties of renal Na⁺,K⁺‐ATPase in both genders of rats. General Physiology and Biophysics, 29(3), 266-274. 0231-5882.