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Changes in cellular response to the damage induced in PC-3 prostate cancer cells by proton microbeam irradiation

In: General Physiology and Biophysics, vol. 31, no. 1
Ewelina Lipiec - Anna Wiecheć - Joanna Dulińska-Litewka - Małgorzata Kubica - Janusz Lekki - Zbigniew Stachura - Joanna Wiltowska-Zuber - Wojciech Kwiatek
Detaily:
Rok, strany: 2012, 11 - 18
O článku:
The aim of this research was to find out whether the passage number effect may influence on the PC-3 cells (the human prostate cancer line derived from bone metastases) response to proton radiation. 2 MeV horizontally focused proton microbeam was used as a radiation source. The cells were treated with a counted number of H+ ions (50–8000) corresponding to doses of 1.3–209 Gy/cell. For comparison, cell death was also induced by UVC radiation. All cells were stained with Hoechst 33342 and propidium iodide and visualized under a fluorescence microscope. Necrosis was observed at: a) 8000 protons per cell (corresponding to ∼209 Gy/cell) after 2–4 passages, b) 3200 protons per cell (corresponding to ∼84 Gy/cell) for cells after 11–14 passages and c) only 800 protons per cell (corresponding to ∼2 Gy/cell ) after 47–50 passages. Apoptosis was efficiently induced, by protons, only in cells after 50 passages. The results showed that the laboratory conditions affected cellular response of PC-3 cell line to the proton irradiation. The cellular response to the radiation treatment strongly depends on number of passages.
Ako citovať:
ISO 690:
Lipiec, E., Wiecheć, A., Dulińska-Litewka, J., Kubica, M., Lekki, J., Stachura, Z., Wiltowska-Zuber, J., Kwiatek, W. 2012. Changes in cellular response to the damage induced in PC-3 prostate cancer cells by proton microbeam irradiation. In General Physiology and Biophysics, vol. 31, no.1, pp. 11-18. 0231-5882.

APA:
Lipiec, E., Wiecheć, A., Dulińska-Litewka, J., Kubica, M., Lekki, J., Stachura, Z., Wiltowska-Zuber, J., Kwiatek, W. (2012). Changes in cellular response to the damage induced in PC-3 prostate cancer cells by proton microbeam irradiation. General Physiology and Biophysics, 31(1), 11-18. 0231-5882.