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Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats

In: General Physiology and Biophysics, vol. 36, no. 4
Snjezana Romic - Katarina Krskova - Rafal Olszanecki - Lucia Balazova - Viktoria Lory - Goran Koricanac - Miroslava Slamkova - Štefan Zórad
Detaily:
Rok, strany: 2017, 399 - 406
O článku:
Fatty acid (FA) uptake and/or intramuscular triglyceride (TG) accumulation in skeletal muscle are increased in obesity, type 2 diabetes and aging. FA translocase (FAT/CD36) translocation, lipin-1 subcellular localization and nuclear factor kappa B (NF-κB) p65 protein content in quadriceps muscle of young and old obese Zucker fa/fa rats and their lean controls were analyzed by immunoblot to define obesity- and aging-related alterations in FA uptake, their subsequent metabolic fate and potential to activate pro-inflammatory signaling. As expected, obesity increased FAT/CD36 content in plasma membrane in quadriceps muscle of fa/fa rats. Aging increased cytosolic lipin-1 content in both, obese rats and their lean controls. Also, old obese rats had decreased level of nuclear extract lipin-1compared to that in old lean rats. Neither obesity nor age altered NF-κB p65 protein content in cytosol and nuclear extract of quadriceps muscle suggesting that obesity/aging-induced changes in FA handling are not accompanied by NF-κB-mediated inflammation. Increase in plasma membrane FAT/CD36 content in obese rats and failure in lipin-1 export to nucleus with progression of obesity, implying an increase in FA uptake and their different channeling into lipid intermediates synthesis pathway in old fa/fa rats versus FA usage in lean rats of the same age.
Ako citovať:
ISO 690:
Romic, S., Krskova, K., Olszanecki, R., Balazova, L., Lory, V., Koricanac, G., Slamkova, M., Zórad, Š. 2017. Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats. In General Physiology and Biophysics, vol. 36, no.4, pp. 399-406. 0231-5882.

APA:
Romic, S., Krskova, K., Olszanecki, R., Balazova, L., Lory, V., Koricanac, G., Slamkova, M., Zórad, Š. (2017). Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats. General Physiology and Biophysics, 36(4), 399-406. 0231-5882.