Sonodynamic therapy induces apoptosis of human leukemia HL-60 cells in the presence of protoporphyrin IX

In: General Physiology and Biophysics, vol. 35, no. 2

Xiaomin Su - Xiaobing Wang - Kun Zhang - Shuang Yang - Quanhong Liu - Albert Leung - Chuanshan Xu - Pan Wang

Detaily:

Rok, strany: 2016, 155 - 164

O článku:

Sonodynamic therapy (SDT) is expected to be a novel therapeutic strategy for tumor. The protoporphyrin IX disodium salt (PpIX), a photosensitizer, can be activated by ultrasound. The present study aims to investigate apoptosis of HL-60 cells induced by PpIX-mediated SDT. 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was adopted to examine cell toxicity. Apoptosis was detected using Annexin V-PE/7-amino-actinomycin D (7-AAD) double staining. Detection of apoptotic bodies was examined by Hoechst33342 (HO) staining. Western blotting was used to analyze the protein of caspase-3 and poly ADP-ribose polymerase (PARP). Intracellular reactive oxygen species (ROS) was detected by a ﬂow cytometer after exposures. Compared with PpIX alone and ultrasound alone groups, the synergistic cytotoxicity of PpIX plus ultrasound were significantly boosted. In addition, as determined by Annexin V-PE/7-AAD staining, SDT significantly induced HL-60 cell apoptosis, the obvious nuclear condensation was also found with HO staining at 4 hours post-SDT treatment. Furthermore, Western blotting showed visible enhancement of caspase-3 and PARP cleavage in this process. Besides, intracellular ROS production was significantly enhanced after SDT. Our findings demonstrate that PpIX-mediated SDT could induce apoptosis on HL-60 cells, suggesting that apoptosis is an important mechanism of cell death induced by PpIX-mediated SDT.

Ako citovať:

ISO 690:

Su, X., Wang, X., Zhang, K., Yang, S., Liu, Q., Leung, A., Xu, C., Wang, P. 2016. Sonodynamic therapy induces apoptosis of human leukemia HL-60 cells in the presence of protoporphyrin IX. In General Physiology and Biophysics, vol. 35, no.2, pp. 155-164. 0231-5882.

APA:

Su, X., Wang, X., Zhang, K., Yang, S., Liu, Q., Leung, A., Xu, C., Wang, P. (2016). Sonodynamic therapy induces apoptosis of human leukemia HL-60 cells in the presence of protoporphyrin IX. General Physiology and Biophysics, 35(2), 155-164. 0231-5882.