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Institute: Institute of Molecular Biology

Raw starch degrading amylases - structure/function and evolution
Amylázy degradujúce surový škrob - štruktúra/funkcia a evolúcia
Program: Bilateral - other
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2012 - 31.12.2018

Amylolytic enzymes involved in degradation of starchy substrates
Amylolytické enzýmy zapojené do degradácie surového škrobu
Program: Bilateral - other
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2012 - 31.12.2018

Evolution of alpha-amylases and related enzymes
Evolúcia alfa-amyláz a príbuzných enzýmov
Program: Bilateral - other
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2015 - 31.12.2019

Rewiring the Streptomyces cell factory for cost-effective production of biomolecules
Nové prepojenie bunkovej továrne Streptomyces pre efektívnu produkciu biomolekúl
Program: FP7
Project leader: RNDr. Kormanec Ján DrSc.
Annotation:STREPSYNTH aims to set-up a Streptomyces-based new industrial production platform (SNIP) for high value added biomolecules. Streptomyces lividans was chosen as a bacterial host cell because it has been already shown to be highly efficient for the extracellular production of a number of heterologous molecules that vary chemically, has a robust tradition of industrial fermentation and is fully accessible to genetic intervention. To develop SNIP our strategy has two components: first, we will construct a collection of reduced-genome S. lividans strains. This will metabolically streamline the cell and rid it of agents (e.g. proteases) of potential harm to the heterologous polypeptides. Second, we will engineer synthetic parts and cassettes, i.e. reshuffled, rewired and repurposed genetic elements either indigenous to S. lividans or heterologous genes organized in artificial operon clusters. These elements will serve three aims: transcriptional and translational optimization, sophisticated on-demand transcriptional regulation that will provide unique fermentation control and metabolic engineering of complete cellular pathways channeling biomolecules to profuse extracellular secretion. Synthetic parts and cassettes will be either directly incorporated into the genome or be hosted in the form of plasmids. Systems biology tools will guide fine-tuning rounds of cell factory engineering and fermentation optimization. To set up SNIP we chose two classes of biomolecules with obvious immediate industrial value and application: heterologous proteins (industrial enzymes, biopharmaceuticals, biofuel enzymes, diagnostics) and small molecules (lantipeptides and indolocarbozoles) useful for multiple industrial purposes (biopharmaceuticals, additives, food technology, bioenergy). These biomolecules are of immediate interest to SMEs that participate and guide the industrial relevance of STREPSYNTH. SNIP is a modular platform that can be repurposed for diverse future applications.
Duration: 1.12.2013 - 30.11.2018

Understanding Movement and Mechanism in Molecular Machines
Pochopenie pohybu a mechanizmu molekulárnych mašín
Program: COST
Project leader: RNDr. Urbániková Ľubica CSc.
Annotation:Structural biology has seen tremendous progress in the elucidation of static atomic structures of increasingly complex biomolecular systems, many of which are potential targets for future molecular medicine and biotechnology applications. However, understanding of biomolecular mechanism and function also requires knowledge of dynamics, both locally at active sites and more globally in terms of long range domain movements. These dynamic aspects are fundamentally important but difficult to study, often requiring both spectroscopic and structural complementary approaches and methods. Recent advances in the development of such methods make it timely to establish an EU network to bring together the spectroscopic and structural biology communities that will enable integration of the static and dynamic aspects of important biomolecules. This Action will promote the collaborations necessary to achieve this integration, and will disseminate knowledge and target research that will ultimately translate into new understanding of complex biochemical processes. Such knowledge will foster insights into disease states and lead to new biotechnology and future drug discoveries. It will consolidate and further enhance the world-leading role of European groups in these areas and provide a platform for training and promoting ESRs, through a tailored program of Summer Schools, Workshops, Short-Term Scientific Missions, and conferences.
Duration: 3.6.2014 - 2.6.2018

Exploring Microbial Diversity and Functionality in Thermophilic Bioreactors for Innovation in Biotechnology
Prieskum diverzity a funkčnosti mikroorganizmov termofilných bioreaktorov za účelom biotechnologických inovácií
Program: Bilateral - other
Project leader: Dr. Pangallo Domenico DrSc.
Duration: 1.1.2015 - 31.12.2017

Protein design and evolution of raw starch digesting amylases
Proteínový dizajn a evolúcia amyláz degradujúcich surový škrob
Program: Bilateral - other
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2015 - 31.12.2019

Structure/function and evolution of amylolytic enzymes
Štruktúra, funkcia a evolúcia amylolytických enzýmov
Program: Bilateral - other
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2016 - 31.12.2020

Structure, function and evolution of trehalose synthases
Štruktúra, funkcia a evolúcia trehalózasyntáz
Program: Bilateral - other
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2015 - 31.12.2019

Roles and Structural Determinants of Low-affinity Carbohydrate-Protein Interactions
Úlohy a štrukturálne determinanty nízkoafinitných interakcií medzi sacharidmi a proteínmi
Program: Multilateral - other
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.9.2016 - 31.8.2018

The total number of projects: 10