Information Page of SAS Organisation

Institute of Normal and Pathological Physiology

National projects

Effect of lyophylisate Cornus mas L. on cardiometabolic and inflammatory parameters in experimental metabolic syndrome
Efekt lyofilizátu Cornus mas L. na kardiometabolické a zápalové parametre pri experimentálnom metabolickom syndróme
Program: VEGA
Project leader: Doc.MUDr. Lietava Ján CSc.
Duration: 1.1.2016 - 31.12.2018

NOSCHIZ - Interaction of nitrergic, neurotrophic and endocrine signaling in the etiopathogenesis of schizophrenia
Interakcia nitrergickej, neurotrofickej a endokrinnej signalizácie v etiopatogenéze schizofrénie
Program: APVV
Project leader: MUDr. Riečanský Igor PhD.
Annotation:Schizophrenia is a common and severe mental disorder but its pathogenesis is yet poorly understood. Susceptibility to schizophrenia is largely genetic but the genetic predisposition is determined in a complex network of interactions between multiple risk genes and environmental factors, resulting in disordered brain development and function. There is increasing evidence that chronic stress and dysregulation of several signaling pathways plays role in the neurodevelopmental impairment underlying schizophrenia. In this project, by adopting a multidisciplinary approach, we will address at several levels (genetic, neurobiological and behavioral) a candidate pathophysiological pathway, involving nitrergic, neurotrophic and stress signaling, which might be importantly involved in the disordered brain development in schizophrenia. A focus on schizophrenia endophenotypes will enable us to integrate findings from human subjects at genetic risk of the disorder with those from a rodent neurodevelopmental model of schizophrenia. This project will bring important new knowledge on the etiopathogenesis of this devastating disorder and potential novel strategies of its treatment.
Duration: 1.7.2015 - 30.6.2019

KANASTA - Cardiovascular Effects of Nanoencapsulated Simvastain and Coenzyme Q10 in Experimental Hyperlipidemia (KANASTA)
Kardiovaskulárne účinky nanoenkapsulovaného simvastatínu a koenzýmu Q10 pri experimentálnej hyperlipidémii (KANASTA)
Program: Iné projekty
Project leader: RNDr. Reháková Radoslava
Annotation:The project is aimed to investigate cardiovascular effects of simvastatin together with CoQ10 in experimental hyperlipidemia and to increase bioavailability of simvastatin in the liver, thus reducing the daily dose and consequently to prevent the reduction of CoQ10 levels.
Duration: 27.11.2015 - 26.11.2018

Mechanisms involved in uric acid-induced endothelial dysfunction depending on the age and genetic predisposition to hypertension
Mechanizmy zahrnuté v endotelovej dysfunkcii indukovanej kyselinou močovou v závislosti od veku a genetickej predispozície k hypertenzii
Program: VEGA
Project leader: MUDr. RNDr. Púzserová Angelika PhD.
Annotation:Studies have shown a significant relationship between increased concentration of uric acid in the blood (hyperuricaemia) and cardiovascular diseases, including hypertension. But there is little information on the mechanisms by which uric acid can lead to end-organ damage. Hyperuricaemia combined with hypertension is associated with endothelial dysfunction. However, the mechanisms by which hyperuricaemia causes endothelial dysfunction are not clarified. This project aims to clarify the relationship of hyperuricaemia and hypertension, especially in terms of endothelial function. The aim is to bring new results highlighting the impact of elevated concentrations of uric acid on the endothelium, and to reveal the mechanisms involved in endothelial dysfunction in conductive and resistant arteries isolated from peri-pubertal and adult normotensive, prehypertensive and hypertensive rats. The results will contribute to the knowledge about pathophysiology of hyperuricaemia-induced endothelial dysfunction.
Duration: 1.1.2017 - 31.12.2020

Design and implementation of visual biofeedback for the rehabilitation of mobility deficiencies in patients with low back pain
Návrh a implementácia metodiky pre rehabilitáciu pacientov s bolesťami chrbta s využitím zrakového biofeedbacku
Program: APVV
Project leader: Ing. Hlavačka František CSc.
Annotation:The main goal of this project is to design, optimize and implement a specialized method for the improved rehabilitation of mobility deficiencies in patients suffering from low back pain (LBP). The system will be equipped with accurate inertial measuring units embedded with highly sensitive micro-electro-mechanical (MEMS) accelerometers and gyroscopes, as well as a force platform providing input signals that will be processed and displayed back to the patient (visual biofeedback). LBP is a worldwide health problem affecting people of all ages, with recurring symptoms. Based on positive long-term experiences with visual biofeedback, the project strives to design an effective rehabilitation program for the improvement of impaired trunk mobility during sitting, and also impaired balance control during stance in LBP patients. The project not only includes the accurate acquisition of postural data, but also the development of software which will process, interpret and display this data in an easy to understand way. The software is intended to be easily operated and include training tasks that can be personalized to patient specific requirements. The ultimate goal of the project is to implement complex sensor systems capable of accurate measurements but process the data into an easy to use and easy to interpret rehabilitation tool for improving postural and motor function deficiencies in patients suffering from LBP.
Duration: 1.7.2017 - 30.6.2019

NO-NEW-REG - New regulatory effects of nitric oxide and their role in the development of essential hypertension
Nové regulačné účinky oxidu dusnatého a ich úloha v rozvoji esenciálnej hypertenzie
Program: APVV
Project leader: RNDr. Čačányiová Soňa PhD.
Annotation:High blood pressure is a main risk factor in sustained increased morbidity and mortality of humans suffering cardiovascular diseases. Understanding of causes leading to hypertension enable to reveal new preventive and therapeutic decisions. A new regulatory system involved in vessel tree regulation seems to be neuronal NOsynthase (nNOS) and its interactions with other regulatory systems. On the level of the kidney nNOS signal pathway interferes with renin-angiotensin system (RAS) and sulfide signalization (H2S), and the interactions among them are the unexplored area of regulatory mechanisms. nNOS in macula densa stimulate renin syntesis and via it influences RAS and sympathetic nerve system, on the other hand, H2S inhibits renin synthesis. Moreover, the regulatory pathways of nNOS and also endothelial eNOS could interact with endogenous NOS inhibitor, asymmetric dimetylarginine ADMA, on local as well as systemic level. The aim of the project is to study the effect of interactions of NO/nNOS/eNOS signalization with mentioned regulatory pathways (RAS, H2S, ADMA) on cardiovascular system and to find out their role in the specificity of nNOS and/or eNOS action in the conditions of essential hypertension. The availability of the results will be reached via using complex approach (functional, molecular, morphological). Moreover, on the level of acute experiments, we will confront selectedbiochemical markers of perivascular adipose tissue (plasma/serum) as well as vasoactive responses of arteries isolated from normotensive and hypertensive rats with biochemical markers and reactivity of vessels isolated after nephrectomy from normotensive patients and patients with essential hypertension.
Duration: 1.7.2016 - 30.6.2020

Protection of hypertensive and failure heart by I(f) channel blocker ivabradin: comparison with ACE inhibition and melatonin
Protekcia hypertenzného a zlyhávajúceho srdca blokátorom I(f) kanálu ivabradínom: porovnanie s ACE-inhibíciou a melatonímom
Program: VEGA
Project leader: doc. RNDr. Pecháňová Oľga DrSc.
Duration: 1.1.2015 - 31.12.2018

Protective effect of NO and CO donors in experimental myocardial infarction with hypertensive complications
Protektívny účinok NO a CO donorov pri experimentálnom infarkte myokardu s hypertenzívnymi komplikáciami
Program: VEGA
Project leader: doc. RNDr. Pecháňová Oľga DrSc.
Duration: 1.1.2015 - 31.12.2018

ENDBIOM - Study of critical endogenous biomarkers and signaling pathways in hypertension and cardiovascular diseases
Sledovanie kritických endogénnych biomarkerov a signálnych dráh v hypertenzii a pri kardiovaskulárnych ochoreniach
Program: VEGA
Project leader: RNDr. Dovinová Ima PhD.
Annotation:Several endogenous factors and signaling pathways contribute to the development of cardiovascular diseases. Activation of the renin-angiotensin and aldosterone system (RAAS), deteriorated relaxation of vasoactive systems producing NO and H2S, as well as an imbalance of redox pathways producing reactive oxygen and nitrogen species (ROS a RNS). Hypertension is a major risk factors of cardiovascular diseases and leads to functional and structural alterations in blood vessel walls. The landmarks of developed hypertension are increased vasoconstriction regulated by RAAS, oxidative stress manifested in increased ROS and RNS, endothelial dysfunction (increased levels of assymetric dimethyl-arginine - ADMA - and homocystein) as well as cardiovascular remodeling (activation of metaloproteinases). Detection, monitoring, and regulation of the critical pathology markers can lead to a better management of hypertension- and metabolic syndrome-related diseases.
Duration: 1.1.2017 - 31.12.2019

Specific methods and innovative procedures for assessing performance in athletes and physical fitness in the general population
Špecifické metódy a inovované postupy posudzovania výkonnosti športovcov a telesnej zdatnosti bežnej populácie
Program: VEGA
Project leader: RNDr. Bzdúšková Diana PhD.
Annotation:The project will design specific tests and innovative methodological procedures for assessing performance in athletes and physical fitness in the general population. These will be based on analysis of performance in selected sports and age specificities of the general population. Methodology of measurement and data analysis with the highest accuracy of motor abilities evaluation will be verified. Their ability to differentiate between and within groups differences will also be evaluated. Part of the project will be testing guidance scales on groups of elite athletes of selected sports and large population of varied ages. This will provide a basis for testing protocols and recommendations for utilization of novel diagnostic methods in practice. Such diagnostics taking into account specific conditions of particular sports and age specificities represent significant shift in obtaining relevant information on performance in athletes and physical fitness in the general population and their longitudinal changes.
Duration: 1.1.2017 - 31.12.2019

H2S-NO - Study of biological effects of H2S/NO products and molecular mechanism of their actions
Štúdium biologických účinkov produktov H2S/NO interakcie a molekulárne mechanizmy ich pôsobenia
Program: APVV
Project leader: RNDr. Čačányiová Soňa PhD.
Annotation:Now it is well acknowledged that endogenously produced H2S affects and is involved in regulation of many physiological and pathological functions of living organisms. It is suggested that biological effects of H2S might not result from actions of H2S alone, but from its oxidation products, which come from e.g. interaction of H2S with NO. Last four years, our “international” group indentified the following products of H2S and NO interaction: nitrosopersulfide (SSNO−), polysulfides (HSn−) and dinitrososulfite [N-nitrosohydroxylamine-N-sulfonate (SULFI/NO) (Proc Natl Acad Sci U S A. 112, 2015, E4651-E4660). Biological effects and molecular mechanisms of these products are not completely understood Therefore the aim of our project is to explore biological effects of the products of H2S/NO interactions and to study their molecular mechanism of their actions. Particularly, as a continuation of our research, we will study their effects on rat blood pressure and aortic rings relaxation. To elucidate molecular mechanisms of their biological effects, we will study their influence on expression of enzymes that endogenously produce H2S (CBS, CSE and 3-MST), on intracellular membrane channels, concentration of intracellular calcium, lipid peroxidation and their antioxidant properties. Goal of the project is also to find out, if studied compounds could provide us with information leading to a drug design based on their molecular structure, what could be an object for next application studies and lead to implementation in medical praxis.
Duration: 1.7.2016 - 30.6.2020

NANOSIMKA - Effects of nanoencapsulated simvastatin on cardiovascular system in experimental metabolic syndrome
Účinok nanoenkapsulovaného simvastatínu na kardiovaskulárny systém pri experimentálnom metabolickom syndróme
Program: APVV
Project leader: doc. RNDr. Pecháňová Oľga DrSc.
Duration: 1.7.2015 - 30.6.2019

The effect of STAT1 and ISG15 inhibitors on biochemical and morphological parameters in experimental myocardial infarction
Účinok STAT1 a ISG15 inhibítorov na biochemické a morfologické parametre pri experimentálnom infarkte myokardu
Program: VEGA
Project leader: RNDr. Cebová Martina PhD.
Annotation:Myocardial infarction (MI) remains a major cause of morbidity and mortality throughout the world. Atherosclerosis, chronic inflammation and metabolic diseases are the main causes of MI. Hypertension is the most serious risk factors which worsen the prognosis of MI via induction of oxidative and inflammatory mediators. Chronic ischemia leads to irreversible myocardial damage. Reperfusion of myocardium may potentially save myocardial function, paradoxically, this process causes further damage of the myocardium and apoptosis of cardiomyocytes along with upregulation of STAT1 and ISG15. Thus, it is important to study different molecules which may block or revers pathological processes in myocardial reperfusion injury following MI at different levels. Therefore, the aim of our study is to analyze the effects of STAT1 and ISG15 inhibitors on reperfusion injury in the experimental model of MI (ischemia-reperfusion injury after STEMI revascularization) and determination of pathophysiological changes in this process.
Duration: 1.1.2017 - 31.12.2019

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Vplyv endogénnej hladiny oxidu dusnatého a sírovodíka na tlak krvi, pulzovú vlnu, funkciu a štruktúru cievnej steny
Program: VEGA
Project leader: RNDr. Kristek František DrSc.
Duration: 1.1.2017 - 31.12.2020

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Vplyv konštitučných faktorov redoxnej regulácie na endofenotypové znaky schizofrénie
Program: VEGA
Project leader: MUDr. Riečanský Igor PhD.
Duration: 1.1.2016 - 31.12.2018

Vplyv transkraniálnej stimulácie mozgu jednosmerným prúdom na senzorimotorické vrátkovanie u človeka
Vplyv transkraniálnej stimulácie mozgu jednosmerným prúdom na senzorimotorické vrátkovanie u človeka
Program: VEGA
Project leader: MUDr. Jagla Fedor CSc.
Annotation:The term sensorimotor gating refers to a basic inhibitory process, which prevents processing of and reacting to irrelevant stimuli so that resources can be allocated to salient aspects of the environment. Disrupted gating is considered to play a causal role in the development of psychosis in schizophrenia spectrum disorders. Prefrontal cortex (PFC) is involved in inhibitory control processes and its dysfunction is a hallmark of schizophrenia. Transcranial direct current stimulation stimulation (tDCS) can be used to increase or decrease excitability of neuronal tissue and is increasingly used to modulate human brain activity and cognitive processes. In this project, we will systematically explore in healthy adults the possibility to modulate sensorimotor gating by tDCS of the PFC, which has not been investigated so far. Our findings will bring important new knowledge on the brain mechanisms of sensorimotor gating, pathogenesis of mental disorders and possibilities of their treatment.
Duration: 1.1.2017 - 31.12.2019

Effect of ultrasmall superparamagnetic iron oxide nanoparticles on the cardiovascular system of rats with high blood pressure
Vplyv ultra malých superparamagnetických nanočastíc železa na kardiovaskulárny systém potkana v podmienkach vysokého krvného tlaku
Program: VEGA
Project leader: RNDr. Bernátová Iveta DrSc.
Annotation:This project will investigate the effect of ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs) on function and structure of the arterial wall and the heart in rats with high blood pressure. We will investigate if acute stress and chronic high blood pressure can facilitate the USPIONs uptake in the arterial wall and heart, to modify cardiovascular function, including blood pressure regulation and to induce metabolic disorders, oxidative damage and alterations of the Fe2+/Fe3+ ratio in the heart and vasculature. We will investigate if L-type of voltage-dependent calcium channels is involved in iron uptake after USPIONs treatment. Results achieved in this project will contribute to better understanding of USPIONs effects on the cardiovascular system in conditions of acute stress and high blood pressure as well as on prevention of cardiovascular risk resulting from the use of USPIONs in targeted drug delivery.
Duration: 1.1.2017 - 31.12.2020

Age-related changes in sensory control of balance during sit-to-stand and gait
Vplyv veku na senzorickú reguláciu rovnováhy pri vstávaní zo sedu a chôdzi
Program: VEGA
Project leader: Ing. Hlavačka František CSc.
Annotation:The ability to reliably perform functional movements like sit-to-stand, gait or maintaining the erect posture is a basis for the independent living. Difficulties with conducting these motor activities increase with age. Early diagnostics and suitable therapeutic interventions can help to moderate these problems and prevent falls. The goal of our project is to obtain new knowledge about the physiological mechanisms of sit-to-stand movement and gait in young and elderly adults, and also to expand information about the sensory influence on these motor functions. We will focus on analysis of balance control in static and dynamic conditions during sit-to-stand, step initiation and gait using unique 6-camera motion capture system BTS SMART-DX. We assume that results of our project would be worthwhile in designing of perspective rehabilitative methods for elderly people with mobility deficit to improve their balance and functional mobility, and also to enhance diagnostics of early stages of neurological disorders.
Duration: 1.1.2016 - 31.12.2018

RIDD - Research of magnetic forms of iron in development of cardiovascular diseases and behavioural disorders
Výskum magnetických foriem železa v rozvoji kardiovaskulárnych chorôb a porúch správania
Program: APVV
Project leader: RNDr. Bernátová Iveta DrSc.
Annotation:This project proposal is focused on the investigation of the role of iron in development of cardiovascular and behavioural disorders, prevalence of which is increasing during aging. The aim of this project is to investigate the impact of aging on the metabolism of biogenic iron and its magnetic properties in association with metabolic and functional alterations in the cardiovascular system and brain in rats with various genetic predispositions to hypertension. We will determine the molecular biological changes on the level of gene expression, their encoded proteins and the activities of the enzymes involved in the endogenous antioxidant protection, the regulation of nitric oxide production and cell death due to ferroptosis in course of aging. We will also investigate the impact of exogenously administered iron in the form of the biocompatible ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs) on blood pressure regulation and function of the heart and blood vessels in conditions of normotension, chronically increased blood pressure and acute stress (i.e. acutely elevated blood pressure). Results achieved in this project will contribute to better understanding of the effects of the altered iron metabolism, magnetic forms of bodily iron, as well as iron in the form of USPIONs, on the cardiovascular and central nervous systems and to prevention of cardiovascular risk resulting from the use of USPIONs in targeted drug delivery or as the contrast materials for new imaging methods in medicine.
Project web page:http://www.bionanoiron.sav.sk
Duration: 1.7.2017 - 30.6.2021

Research on possibilities and development of SQUID magnetometry for selected applications in biomedicine and material research
Výskum možností a rozvoj SQUID magnetometrie pre vybrané aplikácie v biomedicíne a materiálovom výskume
Program: VEGA
Project leader: RNDr. Bernátová Iveta DrSc.
Annotation:Project has an interdisciplinary character.The aim is to show the possibilities of use of the SQUID magnetometry in study of the actual processes in medicine, biology and material research: -in analysis of the properties and magnetic characterization of the nanoparticles and nanoliquids, especially ultra-small superparamagnetic nanoparticles based on iron oxides (USPIONs) -in investigation of the influence of the USPIONs on the function and structure of the blood vessels and heart, on development of the oxidative damage and in study of processes of the USPIONs transport through cell membranes, blood vessels and organs of rats with normal and high blood pressure -in development of the procedures and methods of quantification of the magnetic substances content in the human and animal cell cultures and organs - in study and development of the aluminate glasses with photoluminescence properties and other applications.
Duration: 1.1.2017 - 31.12.2020

Projects total: 20