Information Page of SAS Organisation

Institute of Molecular Biology

International projects

Raw starch degrading amylases - structure/function and evolution
Amylázy degradujúce surový škrob - štruktúra/funkcia a evolúcia
Program: Bilaterálne - iné
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2012 - 31.12.2018

Amylolytic enzymes involved in degradation of starchy substrates
Amylolytické enzýmy zapojené do degradácie surového škrobu
Program: Bilaterálne - iné
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2012 - 31.12.2018

Evolution of alpha-amylases and related enzymes
Evolúcia alfa-amyláz a príbuzných enzýmov
Program: Bilaterálne - iné
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2015 - 31.12.2019

STREPSYNTH - Rewiring the Streptomyces cell factory for cost-effective production of biomolecules
Nové prepojenie bunkovej továrne Streptomyces pre efektívnu produkciu biomolekúl
Program: 7RP
Project leader: RNDr. Kormanec Ján DrSc.
Annotation:STREPSYNTH aims to set-up a Streptomyces-based new industrial production platform (SNIP) for high value added biomolecules. Streptomyces lividans was chosen as a bacterial host cell because it has been already shown to be highly efficient for the extracellular production of a number of heterologous molecules that vary chemically, has a robust tradition of industrial fermentation and is fully accessible to genetic intervention. To develop SNIP our strategy has two components: first, we will construct a collection of reduced-genome S. lividans strains. This will metabolically streamline the cell and rid it of agents (e.g. proteases) of potential harm to the heterologous polypeptides. Second, we will engineer synthetic parts and cassettes, i.e. reshuffled, rewired and repurposed genetic elements either indigenous to S. lividans or heterologous genes organized in artificial operon clusters. These elements will serve three aims: transcriptional and translational optimization, sophisticated on-demand transcriptional regulation that will provide unique fermentation control and metabolic engineering of complete cellular pathways channeling biomolecules to profuse extracellular secretion. Synthetic parts and cassettes will be either directly incorporated into the genome or be hosted in the form of plasmids. Systems biology tools will guide fine-tuning rounds of cell factory engineering and fermentation optimization. To set up SNIP we chose two classes of biomolecules with obvious immediate industrial value and application: heterologous proteins (industrial enzymes, biopharmaceuticals, biofuel enzymes, diagnostics) and small molecules (lantipeptides and indolocarbozoles) useful for multiple industrial purposes (biopharmaceuticals, additives, food technology, bioenergy). These biomolecules are of immediate interest to SMEs that participate and guide the industrial relevance of STREPSYNTH. SNIP is a modular platform that can be repurposed for diverse future applications.
Project web page:http://rega.kuleuven.be/bac/Funding/strepsynth
Duration: 1.12.2013 - 30.11.2018

Understanding Movement and Mechanism in Molecular Machines
Pochopenie pohybu a mechanizmu molekulárnych mašín
Program: COST
Project leader: RNDr. Urbániková Ľubica CSc.
Annotation:Structural biology has seen tremendous progress in the elucidation of static atomic structures of increasingly complex biomolecular systems, many of which are potential targets for future molecular medicine and biotechnology applications. However, understanding of biomolecular mechanism and function also requires knowledge of dynamics, both locally at active sites and more globally in terms of long range domain movements. These dynamic aspects are fundamentally important but difficult to study, often requiring both spectroscopic and structural complementary approaches and methods. Recent advances in the development of such methods make it timely to establish an EU network to bring together the spectroscopic and structural biology communities that will enable integration of the static and dynamic aspects of important biomolecules. This Action will promote the collaborations necessary to achieve this integration, and will disseminate knowledge and target research that will ultimately translate into new understanding of complex biochemical processes. Such knowledge will foster insights into disease states and lead to new biotechnology and future drug discoveries. It will consolidate and further enhance the world-leading role of European groups in these areas and provide a platform for training and promoting ESRs, through a tailored program of Summer Schools, Workshops, Short-Term Scientific Missions, and conferences.
Project web page:http://www.cost.eu/COST_Actions/cmst/Actions/CM1306
Duration: 3.6.2014 - 2.6.2018

Exploring Microbial Diversity and Functionality in Thermophilic Bioreactors for Innovation in Biotechnology
Prieskum diverzity a funkčnosti mikroorganizmov termofilných bioreaktorov za účelom biotechnologických inovácií
Program: Bilaterálne - iné
Project leader: Dr. Pangallo Domenico DrSc.
Duration: 1.1.2015 - 31.12.2017

Protein design and evolution of raw starch digesting amylases
Proteínový dizajn a evolúcia amyláz degradujúcich surový škrob
Program: Bilaterálne - iné
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2015 - 31.12.2019

Structure/function and evolution of amylolytic enzymes
Štruktúra, funkcia a evolúcia amylolytických enzýmov
Program: Bilaterálne - iné
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2016 - 31.12.2020

Structure, function and evolution of trehalose synthases
Štruktúra, funkcia a evolúcia trehalózasyntáz
Program: Bilaterálne - iné
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2015 - 31.12.2019

Roles and Structural Determinants of Low-affinity Carbohydrate-Protein Interactions
Úlohy a štrukturálne determinanty nízkoafinitných interakcií medzi sacharidmi a proteínmi
Program: Multilaterálne - iné
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.9.2016 - 31.8.2018


National projects

Analysis of the Receptor Binding Proteins of Streptococcal, Staphylococcal, Streptomycetal and Enterococcal phages.
Analýza receptor viažucich proteínov z fágov streptokokov, stafylokokov, streptomycét a enterokokov
Program: VEGA
Project leader: RNDr. Vidová Barbora PhD.
Annotation:The phages specificity is often given by the interaction between the receptor binding proteins of the phage (RBP, tail proteins) and the specific receptors on the host cell surface, making it usable also for the identification of bacteria. Proteomic and bioinformatic studies are useful in the prediction of functional protein for therapeutic intervention. The present project is focused on the in silico study of tail proteins (receptor binding proteins) presence within the selected bacteriophage genomes and experimental analysis of the predicted genes functions. The results extend the knowledge about their presence in genomes, their types, sequence organization, and interaction with components of the cell wall, thus this work opens new perspectives on their use as in phage therapy, as well as to identify the host bacteria.
Duration: 1.1.2016 - 31.12.2019

Antibacterial and immunomodulaotry properties of bee defensin-1 in chronic wound healing process
Antibakteriálne a imunomodulačné vlastnosti včelieho peptidu defenzínu-1 v procese hojenia chronických rán
Program: VEGA
Project leader: Ing. Majtán Juraj PhD.
Duration: 1.1.2014 - 31.12.2017

ATP-dependent proteases and mitochondrial homeostasis
ATP-závislé proteázy a homeostáza mitochondrií
Program: VEGA
Project leader: Ing. Kutejová Eva CSc.
Duration: 1.1.2014 - 31.12.2017

Fylox - Phylogenomic and physiological comparison of oxidative stress responses in thermophilic and mesophilic microbes
Fylogenomický a fyziologický výskum reakcií na oxidačný stres v termofilných a mezofilných mikroorganizmoch.
Program: VEGA
Project leader: RNDr. Zámocký Marcel DrSc.
Annotation:Life on planet Earth developed to its highest levels in the oxygenic atmosphere. This dominating part of biosphere not only supported a more efficient metabolism, but possessed also dangers for aerobically living cells. On the molecular level we can follow the evolution of antioxidant defence mechanisms from ancient cyanobacteria to higher plants & animals. Majority of microbes cope with reactive oxygen species (ROS) during their entire life cycle. H2O2 is physiologically the most abundant ROS. The enzymes catalase & peroxidase evolved for hundreds of million years to fulfill an active defence of organisms against toxic levels of H2O2. Moreover, heme peroxidases evolved further to oxidize aromatic substances forming polymers to give selective advantage for cells expressing them. In our project we will investigate different responses of thermophilic vs. mesophilic microbes to various forms of oxidative stress on molecular & subcellular level. Our findings are expected to have a Great biotechnological impact.
Duration: 1.1.2014 - 31.12.2017

Complex regulation of the stress response and cell differentiation by alternative sigma factors of RNA polymerase in soil Gram-positive bacteria of the genus Streptomyces
Komplexná regulácia odozvy na stres a bunkovej diferenciácie prostredníctvom alternatívnych sigma faktorov RNA polymerázy u pôdnych Gram-pozitívnych baktérií rodu Streptomyces
Program: VEGA
Project leader: RNDr. Kormanec Ján DrSc.
Annotation:In natural habitats, bacteria are exposed to various stresses. Response to the stress is dominantly mediated by alternative stress-response sigma factors of RNA polymerase. The Streptomyces coelicolor genome uniquely encodes nine close homologues of these sigma factors and huge amount of their potential regulators (anti-sigma factors, anti-anti/sigma factors, and signalling PP2C phosphatases). In previous studies, we characterized the role of these sigma factors and showed their dual role in stress response and differentiation, as well as their mutual regulatory interconnection. In addition, we identified promoters recognised by individual sigma factors. The aim of this project is further characterization of the complex regulation of these sigma factors by their regulators in response to stress and differentiation, and investigation of their promoter specificity.
Duration: 1.1.2016 - 31.12.2019

Human milk bioactive glycoprotein lactoferrin as a regulator of homeostasis.
Ľudský mliečny bioaktívny glykoproteín laktoferín ako regulátor homeostázy
Program: VEGA
Project leader: Mgr. Leksa Vladimír PhD.
Annotation:Lactoferrin is present in plasma and in most human exocrine fluids, particularly milk. During inflammation its level in body fluids increases. Lactoferrin exhibits a plethora of biological activities, the best known is its antimicrobial capacity through the iron sequestration. We have discovered that human lactoferrin directly interacts with (i) the mannose 6-phosphate/insulin-like growth factor 2 receptor (CD222), a ubiquitously expressed protein transporter; and (ii) plasminogen, a serine protease central for blood clots dissolution and cell migration. We have found that through these novel interactions lactoferrin modulates cellular and humoral immune responses, and also influences maintenance of homeostasis. The major objective of the presented project proposal is to delineate in detail molecular mechanisms underlying these effects, characterize their physiological relevance, and develop novel pharmacological tools to modulate these processes when they are impaired in inflammatory and cancer diseases.
Duration: 1.1.2017 - 31.12.2020

BryndzaRNA - Metatranscriptome of ewes' lump cheese: An RNA-based approach to determine the contribution of microorganisms to organoleptic quality of bryndza cheese
Metatranskriptóm ovčieho hrudkového syra: RNA-prístup na určenie príspevku mikroorganizmov k organoleptickej kvalite bryndze
Program: APVV
Project leader: Dr. Pangallo Domenico DrSc.
Duration: 1.7.2015 - 30.6.2018

MOPODEG - Modified polymers from renewable resources and their degradation
Modifikované polyméry z obnoviteľných zdrojov a ich degradácia
Program: APVV
Project leader: Dr. Pangallo Domenico DrSc.
Duration: 1.7.2016 - 1.4.2020

IMMUNOMOD - Immune modulation by cytomegalovirus and its immunotherapeutic potential
Modulácia imunitnej odpovede cytomegalovírusom a jej imunoterapeutický potenciál
Program: APVV
Project leader: Ing. Kutejová Eva CSc.
Duration: 1.7.2015 - 30.6.2019

Peptidoglycan hydrolases in streptomycete genomes and their phages
PEPTIDOGLAKANOVÉ HYDROLAZY V GENÓMOCH STREPTOMYCÉT A ICH FÁGOV
Program: VEGA
Project leader: RNDr. Farkašovská Jarmila CSc.
Annotation:The aim of this project is to understand the molecular structure and evolutionary relationship of peptidoglycan hydrolases from Streptomyces and their phages. Peptidoglycan hydrolases, termed also lysins or endolysins, have enormous potential as antimicrobials with respect to the increase in antibiotic resistance among pathogenic bacteria. Bioinformatic and proteomic analysis of lysins will provide the knowledge about the presence, modular organization, type of catalytic and cell wall binding domains and interaction of these enzymes with peptidoglycan. Based on these results there is potential for the construction of chimeric enzymes with different catalytic and bacterial specificities with possible future therapeutic and biotechnological applications.
Duration: 1.1.2014 - 31.12.2017

PoMoMiPaPro - Post-translation modifications in mitochondria and their role in pathological processes
Posttranslačné modifikácie v mitochondriách a ich úloha v patologických procesoch
Program: APVV
Project leader: Ing. Kutejová Eva CSc.
Annotation:Mitochondria, semi-autonomous organelles, have long been recognized as the primary source of metabolic energy for eukaryotic cells. They are also involved in calcium homeostasis and are responsible for the biosynthesis of heme and steroids. Recently, several lines of evidence have shown that the mitochondria are the main regulators of many cellular processes and that mitochondrial dysfunction is linked to a number of pathological conditions, including cancer, neurodegeneration, aging, and inflammation. For example, substantial changes in mitochondrial homeostasis have been observed in cancer cells. These changes lead to respiratory chain dysfunction and mitochondrial DNA mutations. Many of these mutations have been linked to the development of metastasis and resistance to conventional chemotherapy drugs. Recent studies have shown that post-translational modification plays a substantial role in mitochondrial stability. Proteases and kinases, two classes of proteins often involved in post-translational modification, are key players in several pathological processes connected with mitochondria. The proposed project undertakes to study the effect of post-translational modification of the human Lon protease on its stability, activity, and interactions with possible substrates. Particular attention will be paid to those proteins responsible for mtDNA stability. Emphasis will be placed on the changes affected by phosphorylation on the characteristics of Lon and its substrates; the structure and function of selected kinases which may act on Lon or its substrates will be characterized.
Duration: 1.7.2016 - 30.6.2020

PPIS - Regulation of Pericellular Proteolysis: From Molecular Mechanisms To Novel Immune Cell Subsets and Therapeutic tools
Regulácia pericelulárnej proteolýzy: od molekulárnych mechanizmov k novým subsetom imunitných buniek a terapeutickým nástrojom
Program: APVV
Project leader: Mgr. Leksa Vladimír PhD.
Annotation:Plasminogen system is one of the major proteolytic pathways. It is harnessed in many physiological processes, particularly in immune responses. The overall objective of the project is to delineate novel molecular mechanisms that control plasminogen activation in both adaptive and innate immune responses, namely in T cell migration, cytokine activation and homeostasis maintenance by macrophages. The project stems not only from our published data but also from our recent unpublished discoveries and solid preliminary results. Specific goals of the project are not mutually exclusive; there is substantial integration not only at the level of underlying cellular and molecular mechanisms and targets, but also at the level of experimental approaches, tools and devices. The major deliverables of the project will not only include deeper understanding of molecular mechanisms underlying plasminogen activation and characterization of immune cells employing these mechanisms, namely subsets of T cells and macrophages; but also novel molecular tools to pharmacologically modulate the imbalanced plasminogen activation which is associated with many human pathologies, such as chronic inflammatory diseases or cancer. The project achievements will be definitely of interest for scientists in basic research, but also for medical doctors and pharmacological companies.
Duration: 1.7.2017 - 30.6.2021

The replisome of corynephage BFK20 - study of phage replication proteins.
Replizóm korynefága BFK20 - štúdium fágových replikačných proteínov.
Program: VEGA
Project leader: RNDr. Bukovská Gabriela CSc.
Annotation:Our model system for study of essential lytic and replication processes is corynephage BFK20, the lytic phage of industrial strain Brevibacterium flavum CCM 251. The aim of our project is elucidation of corynephage BFK20 replisome structure, the study of phage replication proteins with focus on putative two helicases and polymerase and explanation their participation at DNA replication of phage BFK20 genome. Within the extent of our project we intend to solve the questions which of the host´s proteins are involved in phage replication process. Concurrently, we could acquire the knowledge about the character of specific protein-protein interactions for bacteriophages. The solving of the project will be a contribution to the understanding of bacteriophage replication mechanism and to study of new phage replication proteins.
Duration: 1.1.2014 - 31.12.2017

Bacillus subtilis spore coat – study of formation and self assembling properties of spore coat proteins.
Spórový obal Bacillus subtilis – štúdium tvorby a samo-organizujúcich sa vlastností bielkovín spórového obalu.
Program: VEGA
Project leader: Ing. Krajčíková Daniela CSc.
Annotation:This project is focused on the study of basic principles that govern the formation of spore coat, the outermost proteinaceous shell formed around the endospores of Bacillus subtilis. Spores are formed inside the mother cell cytoplasm as a result of nutritional deprivation. They are capable to withstand extreme conditions exhibiting remarkable resistance properties. The major role in spore resistance plays the spore coat, composed of more than 70 proteins which represents one of the most intricate biological structures. Exploring how the coat is self-assembling bring the knowledge about formation of complex biological arrays to another level. Our research will be pertained to several areas of interest including investigation of protein interactions within the coat and studying the biochemical and biophysical properties of proteins, particularly with morphogenetic function in coat formation. Additionally our experiments will be extended for the effort to get structural information of the key coat proteins.
Duration: 1.1.2014 - 31.12.2017

Synper - Synthetic biology and production of peroxidases de novo
Syntetická biológia a produkcia peroxidáz de novo
Program: APVV
Project leader: RNDr. Zámocký Marcel DrSc.
Annotation:Synthetic biology is recently widely a quickly developing scientific discipline in border of biotechnology and molecular biology with exploitation of knowledge of systems biology (so called omics). In this project we will focuse to synthetic biology/reproducible production of hydroperoxidases, which have main using in enzymatic polymerization reactions as an advantageous alternative to classical organic synthesis to avoid environmental contaminations and also with lower energy costs. We will use our long year experience with construction and analysis of recombinant DNA as well as with heterologous protein expression in bacteria and/or yeasts.
Duration: 1.7.2015 - 30.6.2019

Syntetická bioló - Synthetic biology for the production of new biologically active compounds in streptomycetes
Syntetická biológia pre produkciu nových biologicky aktívnych látok u streptomycét
Program: APVV
Project leader: RNDr. Kormanec Ján DrSc.
Annotation:Bacteria of the genus Streptomyces produce large amount of secondary metabolites, including the majority of antibiotics. Genes for secondary metabolites are located on physically clustered regions. Previously, we identified a gene cluster for antibiotic auricin in Streptomyces aureofaciens CCM3239, which was located on a large stable linear plasmid pSA3239. Sequence analysis of the cluster revealed huge amount of regulatory genes. Their analysis indicated a complex regulation that is responsible for an exceptional production of auricin in a narrow interval of growth. Structural analysis of auricin and the phenotypic analysis of its biosynthetic genes revealed unique mechanism of auricin biosynthesis. The main aim of the project is application of synthetic biology for production of new biologically active compounds in Streptomyces using the biosynthetic genes from the auricin cluster and other polyketide antibiotic clusters. For this purpose the genomic, transcriptomic, and phenotypic analysis of the strain S. aureofaciens CCM3239 will be done, which will reveal new gene clusters for biologically active compounds, as well as strong regulated promoters for their application in synthetic biology. Likewise, new regulatory circuits will be investigated using regulatory genes and promoters from the auricin cluster, as well as strong promoters identified by transcriptomic analysis for their application in synthetic biology. In addition, new vectors on the basis of the large linear plasmid pSA3239 will be prepared to introduce large gene clusters for biologically active compounds into Streptomyses strains for their high and stable production. The results will contribute to the knowledge about regulation of secondary metabolites and may reveal new biologically active compounds with efficient therapeutic properties. Likewise, our results may be helpful in the stable and increased production of clinically important biologically active compounds (antibiotics, cytostatic agents).
Duration: 1.7.2016 - 31.12.2019

Structure-function study of CE16 acetylesterase from Hypocrea jecorina
Štruktúra a funkcia acetylesterázy CE16 z Hypocrea jecorina
Program: VEGA
Project leader: RNDr. Urbániková Ľubica CSc.
Annotation:Acetylesterase from Hypocrea jecorina is a component of the enzyme cocktail produced by the fungus during growth on cellulose. The proposed enzyme role seems to be deacetylation in xylopyranosides preferentially positions 3 and 4. The enzyme shows the unique enzymatic properties and is the first characterised member of the newly established and so far poorly studied carbohydrate esterases family 16. The proposed project is oriented on structural research of the enzyme, search of aminoacid residues important for substrate binding and cleavage, design the point mutations and its structure-function analysis. Obtained results will contribute to our knowledge of structural principles of the studied acetylesterase activity and specificity.
Duration: 1.1.2014 - 31.12.2017

Structural and functional studies on human ryanodine receptor 2 RIH domains together with ligands for the development of new drugs to treat cardiac arrhythmias.
Štruktúra a funkcia RIH domén ľudského ryanodínového receptora 2, ich interakcii s ligandami ako základ pre vývoj liečiv v terapii srdcových arytmií.
Program: VEGA
Project leader: Mgr. Bauerová Vladena PhD.
Annotation:Ryanodine receptor 2 is a calcium channel which releases Ca2+ from the sarcoplasmic reticulum (SR) into the cytoplasm of myocytes, thereby triggering regular heart contraction. Several mutations have been identified in the ryr2 gene which disturb the proper functioning of this channel, leading to life threatening heart arrhythmias. Recent investigations have suggested that dantrolene and K201/JTV519 may correct the abnormal RyR2-mediated Ca2+ release from SR associated with heart failure. The main aim of this project is to characterize in more detail the interactions between dantrolene and K201/JTV519 and their binding sites, which are located on the RyR2 RIH domains. This would provide a deeper understanding of how these drugs exert their effects at a molecular level, and lay a foundation for the design of new, more efficient drugs for the treatment of cardiac arrhythmias.
Duration: 1.1.2016 - 31.12.2019

Formation and regulation of higher-order septin structures
Tvorba a regulácia štruktúr vyššieho poriadku septínov
Program: VEGA
Project leader: RNDr. Farkašovský Marian CSc.
Annotation:Septins are conserved guanosine phosphate–binding proteins involved in cytokinesis and other essential membrane remodeling functions. They form heterooligomeric complexes, which polymerize into nonpolar filaments and are found in all eukaryotes, except plants. Septin structures at division plane serve as a scaffold for the localized assembly of various proteins and as diffusion barrier for membrane-associated proteins. These proteins generate different higher-order structures depending on particular stage of the cell cycle. The mechanisms of formation and localization of septin structures determine also their functionality. Posttranslational modifications and interacting partners are widely accepted regulators of septin filament function. Gic1/2, Bni5,Cdc42 and particular kinases are important for the initial septin ring, S/G2 “hour-glass” and double ring structure formation, respectively. We will focus in our project on the role of these proteins in assembly of septin complex to higher order structures.
Duration: 1.1.2015 - 31.12.2018

INTERLIPID - The role of organelle interactions in lipid homeostasis
Úloha medziorganelových interakcií v lipidovej homeostáze
Program: APVV
Project leader: RNDr. Pevala Vladimír PhD.
Duration: 1.7.2016 - 30.6.2020

SPORDIV - Assembly of protein complexes during asymmetric cell division in sporulating cells of Bacillus subtilis.
Vytváranie proteínových komplexov počas asymetrického bunkového delenia v sporulujúcich bunkách Bacillus subtilis.
Program: APVV
Project leader: RNDr. Barák Imrich DrSc.
Annotation:Bacillus subtilis is an internationally-recognized model organism, whose physiology, biochemistry and genetics has been studied for many years. This organism is used for decades to study the mechanisms of cell division and especially differentiation process, called sporulation. During this process very resistant spores are developed which can lie dormant and then germinate. This resistance of spores presents threats as well as potential benefits to human health and welfare. Botulism and tetanus are infectious diseases transmitted by spores, while spores of Clostridium difficile are responsible for hospital-acquired infections that are harmful to patients and which are expensive to treat and eradicate. Spores of Bacillus cereus cause food poisoning and present a challenge to the food industry, while spores of B. anthracis, which causes anthrax, are a concern because of their potential use as agents of bioterrorism and biowarfare. In contrast, the durability of spores has seen their development for use in nano-biotechnology and as probiotics in the food industry. Meanwhile their temperature resistance and capacity to resist dessication is leading to the development of spores as delivery systems for vaccines. Despite intensive research in the field of B. subtilis sporulation, there are still crucial molecular details missing in the complex mosaic of this process. This project proposes to study the mechanisms of early stages of sporulation in B. subtilis, especially asymmetric septum formation and engulfment. The aims of the project should be achieved by employing wide range of molecular biology, biochemical, genetic, structural biology and most advanced microscopy techniques.
Duration: 1.7.2015 - 30.6.2019

Web atlas of neuroendocrine system in insect
Webový atlas neuroendokrinného systému hmyzu
Program: VEGA
Project leader: Mgr. Stano Matej PhD.
Annotation:Neuroendocrine system of insect is a complex structure formed by specialised cells and organs, which regulate development, behaviour, reproduction and other physiological functions. Crucial signal molecules in the neuroendocrine system are peptides and derivates of amino acids (biogenic amines) and fats (ecdysteroids and juvenile hormones). The goal of this project is to extend and summarise current knowledge in insect's neuroendocrinology. The silkworm (Bombyx mori) will be employed as a model organism. The main result will be an interactive web atlas providing information on individual components of neuroendocrine system, their properties, functions, dynamics and mutual relations. It will cover neuropeptides, biogenic amines, steroid hormones, juvenile hormones, their receptors and synthesizing enzymes. Moreover,it will contain image documentation regarding cellular distribution of signal molecules and their receptors. This atlas will be accessible for scientists around the world through the web portal.
Duration: 1.1.2015 - 31.12.2017

Projects total: 32