Information Page of SAS Organisation

Institute of Molecular Biology

International projects

Raw starch degrading amylases - structure/function and evolution
Amylázy degradujúce surový škrob - štruktúra/funkcia a evolúcia
Program: Bilaterálne - iné
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2012 - 31.12.2018

Amylolytic enzymes involved in degradation of starchy substrates
Amylolytické enzýmy zapojené do degradácie surového škrobu
Program: Bilaterálne - iné
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2012 - 31.12.2018

Evolution of alpha-amylases and related enzymes
Evolúcia alfa-amyláz a príbuzných enzýmov
Program: Bilaterálne - iné
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2015 - 31.12.2019

STREPSYNTH - Rewiring the Streptomyces cell factory for cost-effective production of biomolecules
Nové prepojenie bunkovej továrne Streptomyces pre efektívnu produkciu biomolekúl
Program: 7RP
Project leader: RNDr. Kormanec Ján DrSc.
Annotation:STREPSYNTH aims to set-up a Streptomyces-based new industrial production platform (SNIP) for high value added biomolecules. Streptomyces lividans was chosen as a bacterial host cell because it has been already shown to be highly efficient for the extracellular production of a number of heterologous molecules that vary chemically, has a robust tradition of industrial fermentation and is fully accessible to genetic intervention. To develop SNIP our strategy has two components: first, we will construct a collection of reduced-genome S. lividans strains. This will metabolically streamline the cell and rid it of agents (e.g. proteases) of potential harm to the heterologous polypeptides. Second, we will engineer synthetic parts and cassettes, i.e. reshuffled, rewired and repurposed genetic elements either indigenous to S. lividans or heterologous genes organized in artificial operon clusters. These elements will serve three aims: transcriptional and translational optimization, sophisticated on-demand transcriptional regulation that will provide unique fermentation control and metabolic engineering of complete cellular pathways channeling biomolecules to profuse extracellular secretion. Synthetic parts and cassettes will be either directly incorporated into the genome or be hosted in the form of plasmids. Systems biology tools will guide fine-tuning rounds of cell factory engineering and fermentation optimization. To set up SNIP we chose two classes of biomolecules with obvious immediate industrial value and application: heterologous proteins (industrial enzymes, biopharmaceuticals, biofuel enzymes, diagnostics) and small molecules (lantipeptides and indolocarbozoles) useful for multiple industrial purposes (biopharmaceuticals, additives, food technology, bioenergy). These biomolecules are of immediate interest to SMEs that participate and guide the industrial relevance of STREPSYNTH. SNIP is a modular platform that can be repurposed for diverse future applications.
Project web page:http://rega.kuleuven.be/bac/Funding/strepsynth
Duration: 1.12.2013 - 30.11.2018

Protein design and evolution of raw starch digesting amylases
Proteínový dizajn a evolúcia amyláz degradujúcich surový škrob
Program: Bilaterálne - iné
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2015 - 31.12.2019

Regulatory network of septin higher-order structures.
Regulačná sieť štruktúr vyššieho poriadku septínov.
Program: Bilaterálne - iné
Project leader: RNDr. Farkašovský Marian CSc.
Annotation:Septins are conserved guanosine phosphate–binding proteins involved in cytokinesis and other essential membrane remodelling functions. They form heterooligomeric complexes, which polymerize into nonpolar filaments and are present in most eukaryotic organisms. Septin structures serve as a scaffold for the localized assembly of various proteins at the cell division plane. They also act as diffusion barrier for membrane-associated proteins, which generate different higher-order structures depending on the particular stage of the cell cycle. The mechanisms of formation, localization of septin structures and the regulation of septin ring assembly are difficult to study and therefore poorly understood. Our main goal is to explain how the protein kinases Elm1, Gin4, Cla4 and Cdc28 are involved in the regulation of coordinated assembly of septins in different stages of the cell cycle. We will study the in vitro phosphorylation of GFP-labelled septins by fluorescence microscopy observing the production of kinase reaction products in free solution and on supported lipid bilayer. Cryo electron microscopy and single particle analysis will be employed to obtain structural details of the phosphorylation-induced changes in septins. Observation of the localization and structural changes of the septin cortex in vivo as well as localization of interacting proteins at different conditions and in different genetic backgrounds (e.g. kinase mutants) will support our in vitro findings.
Duration: 1.1.2017 - 31.12.2019

Structure/function and evolution of amylolytic enzymes
Štruktúra, funkcia a evolúcia amylolytických enzýmov
Program: Bilaterálne - iné
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2016 - 31.12.2020

Structure, function and evolution of trehalose synthases
Štruktúra, funkcia a evolúcia trehalózasyntáz
Program: Bilaterálne - iné
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.1.2015 - 31.12.2019

Roles and Structural Determinants of Low-affinity Carbohydrate-Protein Interactions
Úlohy a štrukturálne determinanty nízkoafinitných interakcií medzi sacharidmi a proteínmi
Program: Multilaterálne - iné
Project leader: Doc. Ing. Janeček Štefan DrSc.
Duration: 1.9.2016 - 31.8.2018

FARMAZYM - Development of structural methods to the study of pharmacologically active enzymes
Vývoj štruktúrnych metód na štúdium farmakologicky aktívnych enzýmov
Program: Multilaterálne - iné
Project leader: RNDr. Urbániková Ľubica CSc.
Annotation:This project will establish the first structural biology consortium in the Danube region, creating a regional research cluster between Serbia, Slovakia and the Czech Republic. Multilateral cooperation will foster development of structural biology methods for study of pharmacologically active enzymes important for treatment of human cancers and pathogenic bacterial infections. Specifically we will merge expertise and resources in structural bioinformatics (Serbia), protein crystallization (Slovakia) and X-ray diffraction (Czech Republic) to accomplish our scientific goals at the study of endolysins, carbonic anhydrases and aldoketoreductases. Pharmacologically active enzymes affect the efficacy of clinical drugs by activation/inhibition of drug compounds, improving drug stability or delivery, or exposing new targets of action. By elucidating the molecular basis of these phenomena, this research will impact efforts to overcome drug resistance in human cancers and pathogenic infections.
Duration: 1.1.2017 - 31.12.2018


National projects

Analysis of the Receptor Binding Proteins of Streptococcal, Staphylococcal, Streptomycetal and Enterococcal phages.
Analýza receptor viažucich proteínov z fágov streptokokov, stafylokokov, streptomycét a enterokokov
Program: VEGA
Project leader: RNDr. Vidová Barbora PhD.
Annotation:The phages specificity is often given by the interaction between the receptor binding proteins of the phage (RBP, tail proteins) and the specific receptors on the host cell surface, making it usable also for the identification of bacteria. Proteomic and bioinformatic studies are useful in the prediction of functional protein for therapeutic intervention. The present project is focused on the in silico study of tail proteins (receptor binding proteins) presence within the selected bacteriophage genomes and experimental analysis of the predicted genes functions. The results extend the knowledge about their presence in genomes, their types, sequence organization, and interaction with components of the cell wall, thus this work opens new perspectives on their use as in phage therapy, as well as to identify the host bacteria.
Duration: 1.1.2016 - 31.12.2019

Evolution of amylolytic enzymes
Evolúcia amylolytických enzýmov
Program: VEGA
Project leader: Doc. Ing. Janeček Štefan DrSc.
Annotation:The project is focused on studying the amylolytic enzymes. Starch hydrolases and related alpha-glucan active enzymes are in the centre of attention that, via the retaining mechanism, catalyse the hydrolysis of alpha-glucosidic bonds in starch and related alpha-glucans, and form and modify these linkages by transglycosylations and isomerisations. They cover more than 30 enzyme specificities, e.g. alpha-amylases, cyclodextrin glucanotransferases, alpha-glucosidases, pullulanases, trehalose synthases, etc. They are represented by two families of glycoside hydrolases: (i) GH13 (32,500 sequences; clan GH-H with GH70 and GH77); and (ii) GH57 (1,500 sequences; related with GH119). The main goal of the project is, based on bioinformatics analyses of amylolytic enzymes, to contribute significantly to overall knowledge of biological processes of starch, glycogen and related alpha-glucans metabolism within a widest possible spectrum of living organisms. Results may be potentially used in protein engineering and design.
Duration: 1.1.2017 - 31.12.2020

FUNPOX - Hybrid, lignolytic and versatile heme peroxidases from Ascomycetes and Basidiomycetes
Hybridné, lignolytické a verzatilné hémové peroxidázy z askomycétnych a bazidiomycétnych húb
Program: VEGA
Project leader: RNDr. Zámocký Marcel DrSc.
Duration: 1.1.2018 - 31.12.2021

Characterizations of de novo isolated bacteria and its heavy-metal-resistance determinant from soil contaminated by high nickel content
Charakterizácia novo izolovanej baktérie a jej determinanta rezistencie proti ťažkým kovom z pôdy znečistenej vysokým obsahom niklu
Program: VEGA
Project leader: RNDr. Ferianc Peter CSc.
Annotation:A soil very likely represents the biggest diversity reservoirs not only for particular microbial species but also for genetic codes of useful compounds carried by these microorganisms. Sampling of diverse environments showed that the majority of cells visible under the microscope are left “uncultured”. To resolve this paradox, “the great plate count anomaly”, we have isolated, by using of diffusion chamber, until now an uncultured isolate marked as MR-CH-I15-I [KC809952] from soil contained high concentration of nickel. This isolate was characterised by high level of resistance mainly against nickel, cobalt and cadmium, and carried a resistance determinant against heavy metals encoding CzcA-like protein with similarity lower than 85 %. Only a few bacteria have been isolated from toxic-metal-contaminated environments and had their metabolic pathways studied for the detoxification of pollutants in detail. The aim of the project is the further isolate characterisation and it’s variant of toxic-metal resistance determinants.
Duration: 1.1.2017 - 31.12.2019

CTC - Identification and validation of signalling pathways associated with circulating tumor cells in breast cancer.
Identifikácia a validácia signálnych dráh asociovaných s cirkulujúcimi nádorovými bunkami pri karcinóme prsníka
Program: APVV
Project leader: Mgr. Kľučár Ľuboš PhD.
Annotation:Circulating tumor cells (CTC) are indepent prognostic factor in primary as well as in metastatic breast cancer. CTC are heterogenous population of tumor cells and play crucial role in metastatic cascade and tumor progression in process termed self-seeding. Presence of CTC in peripheral blood is a surrogate marker of tumor metastatic ability. Identification of signalling pathways associated with presense of CTC in peripheral blood could help to identifify new therapeutic targets in breast cancer. This project is aimed to identify biomarkers and subsequently signalling pathways in primary tumor associated with different subsets of CTC using using highthroughput technologies of genomics and biostatistcs through translational research involving the analysis of biological material from patients followed by their prospective validation.
Duration: 1.7.2017 - 30.6.2021

Innovative disinfection strategies: the essential oils effect on microflora and materials of cultural heritage objects
Inovatívne stratégie dezinfekcie: vplyv esenciálnych olejov na mikroflóru a materiály objektov kultúrneho dedičstva
Program: VEGA
Project leader: Dr. Pangallo Domenico DrSc.
Duration: 1.1.2017 - 31.12.2020

Complex regulation of the stress response and cell differentiation by alternative sigma factors of RNA polymerase in soil Gram-positive bacteria of the genus Streptomyces
Komplexná regulácia odozvy na stres a bunkovej diferenciácie prostredníctvom alternatívnych sigma faktorov RNA polymerázy u pôdnych Gram-pozitívnych baktérií rodu Streptomyces
Program: VEGA
Project leader: RNDr. Kormanec Ján DrSc.
Annotation:In natural habitats, bacteria are exposed to various stresses. Response to the stress is dominantly mediated by alternative stress-response sigma factors of RNA polymerase. The Streptomyces coelicolor genome uniquely encodes nine close homologues of these sigma factors and huge amount of their potential regulators (anti-sigma factors, anti-anti/sigma factors, and signalling PP2C phosphatases). In previous studies, we characterized the role of these sigma factors and showed their dual role in stress response and differentiation, as well as their mutual regulatory interconnection. In addition, we identified promoters recognised by individual sigma factors. The aim of this project is further characterization of the complex regulation of these sigma factors by their regulators in response to stress and differentiation, and investigation of their promoter specificity.
Duration: 1.1.2016 - 31.12.2019

Human milk bioactive glycoprotein lactoferrin as a regulator of homeostasis.
Ľudský mliečny bioaktívny glykoproteín laktoferín ako regulátor homeostázy
Program: VEGA
Project leader: Mgr. Leksa Vladimír PhD.
Annotation:Lactoferrin is present in plasma and in most human exocrine fluids, particularly milk. During inflammation its level in body fluids increases. Lactoferrin exhibits a plethora of biological activities, the best known is its antimicrobial capacity through the iron sequestration. We have discovered that human lactoferrin directly interacts with (i) the mannose 6-phosphate/insulin-like growth factor 2 receptor (CD222), a ubiquitously expressed protein transporter; and (ii) plasminogen, a serine protease central for blood clots dissolution and cell migration. We have found that through these novel interactions lactoferrin modulates cellular and humoral immune responses, and also influences maintenance of homeostasis. The major objective of the presented project proposal is to delineate in detail molecular mechanisms underlying these effects, characterize their physiological relevance, and develop novel pharmacological tools to modulate these processes when they are impaired in inflammatory and cancer diseases.
Duration: 1.1.2017 - 31.12.2020

Mechanisms of asymmetric cell division during sporulation of Bacillus subtilis
Mechanizmy asymetrického bunkového delenia počas sporulácie Bacillus subtilis
Program: VEGA
Project leader: RNDr. Barák Imrich DrSc.
Annotation:Bacillus subtilis is an internationally-recognized model organism, whose physiology, biochemistry and genetics has been studied for many years. Under nutrient rich conditions, this bacterium grows and multiplies by division at mid-cell. B. subtilis belongs to the bacteria which has the capacity to form dormant cell forms called spores. The capacity of spores to lie dormant and then germinate presents threats as well as potential benefits to human health and welfare. Many serious infectious diseases as botulism, tetanus, food poisoning and antrax are infectious diseases transmitted by spores. In contrast, the durability of spores has seen their development for use in nano-biotechnologies and in the food industry. Temperature resistance of B. subtilis spores and their capacity to resist desiccation is leading to the development of spores as delivery systems for vaccines. This is also a reason why this organism is used for decades to study the mechanisms of cell division and especially sporulation.
Duration: 1.1.2017 - 30.12.2020

MOPODEG - Modified polymers from renewable resources and their degradation
Modifikované polyméry z obnoviteľných zdrojov a ich degradácia
Program: APVV
Project leader: Dr. Pangallo Domenico DrSc.
Duration: 1.7.2016 - 1.4.2020

IMMUNOMOD - Immune modulation by cytomegalovirus and its immunotherapeutic potential
Modulácia imunitnej odpovede cytomegalovírusom a jej imunoterapeutický potenciál
Program: APVV
Project leader: Ing. Kutejová Eva CSc.
Duration: 1.7.2015 - 30.6.2019

PoMoMiPaPro - Post-translation modifications in mitochondria and their role in pathological processes
Posttranslačné modifikácie v mitochondriách a ich úloha v patologických procesoch
Program: APVV
Project leader: Ing. Kutejová Eva CSc.
Annotation:Mitochondria, semi-autonomous organelles, have long been recognized as the primary source of metabolic energy for eukaryotic cells. They are also involved in calcium homeostasis and are responsible for the biosynthesis of heme and steroids. Recently, several lines of evidence have shown that the mitochondria are the main regulators of many cellular processes and that mitochondrial dysfunction is linked to a number of pathological conditions, including cancer, neurodegeneration, aging, and inflammation. For example, substantial changes in mitochondrial homeostasis have been observed in cancer cells. These changes lead to respiratory chain dysfunction and mitochondrial DNA mutations. Many of these mutations have been linked to the development of metastasis and resistance to conventional chemotherapy drugs. Recent studies have shown that post-translational modification plays a substantial role in mitochondrial stability. Proteases and kinases, two classes of proteins often involved in post-translational modification, are key players in several pathological processes connected with mitochondria. The proposed project undertakes to study the effect of post-translational modification of the human Lon protease on its stability, activity, and interactions with possible substrates. Particular attention will be paid to those proteins responsible for mtDNA stability. Emphasis will be placed on the changes affected by phosphorylation on the characteristics of Lon and its substrates; the structure and function of selected kinases which may act on Lon or its substrates will be characterized.
Duration: 1.7.2016 - 30.6.2020

Terafag - Bacteriophage preparations for therapy of vaginal and urinary infection
Príprava bakteriofágov na terapiu vaginálnych a močových infekcií
Program: APVV
Project leader: RNDr. Bukovská Gabriela CSc.
Annotation:The increasing occurrence of antibiotic-resistant pathogens is a major problem in current health care.Bacteriophages are viruses capable to kill bacteria and therefore have a great potential to be used in the treatment of infectious diseases, especially in the case of resistant bacteria. The lack of knowledge about the phage biology previously often led to disappointing results. Therefore, a comprehensive study of the therapeutic phage properties is a prerequisite for their full application potential. In this project, we will isolate and characterize bacteriophages specific for selected bacterial pathogens which are causative agents of urinary tract infections or which potentially threaten newborns. We will prepare a phage cocktail which will be effective in eliminating of model pathogens.
Duration: 1.7.2017 - 30.6.2021

PPIS - Regulation of Pericellular Proteolysis: From Molecular Mechanisms To Novel Immune Cell Subsets and Therapeutic tools
Regulácia pericelulárnej proteolýzy: od molekulárnych mechanizmov k novým subsetom imunitných buniek a terapeutickým nástrojom
Program: APVV
Project leader: Mgr. Leksa Vladimír PhD.
Annotation:Plasminogen system is one of the major proteolytic pathways. It is harnessed in many physiological processes, particularly in immune responses. The overall objective of the project is to delineate novel molecular mechanisms that control plasminogen activation in both adaptive and innate immune responses, namely in T cell migration, cytokine activation and homeostasis maintenance by macrophages. The project stems not only from our published data but also from our recent unpublished discoveries and solid preliminary results. Specific goals of the project are not mutually exclusive; there is substantial integration not only at the level of underlying cellular and molecular mechanisms and targets, but also at the level of experimental approaches, tools and devices. The major deliverables of the project will not only include deeper understanding of molecular mechanisms underlying plasminogen activation and characterization of immune cells employing these mechanisms, namely subsets of T cells and macrophages; but also novel molecular tools to pharmacologically modulate the imbalanced plasminogen activation which is associated with many human pathologies, such as chronic inflammatory diseases or cancer. The project achievements will be definitely of interest for scientists in basic research, but also for medical doctors and pharmacological companies.
Duration: 1.7.2017 - 30.6.2021

Synper - Synthetic biology and production of peroxidases de novo
Syntetická biológia a produkcia peroxidáz de novo
Program: APVV
Project leader: RNDr. Zámocký Marcel DrSc.
Annotation:Synthetic biology is recently widely a quickly developing scientific discipline in border of biotechnology and molecular biology with exploitation of knowledge of systems biology (so called omics). In this project we will focuse to synthetic biology/reproducible production of hydroperoxidases, which have main using in enzymatic polymerization reactions as an advantageous alternative to classical organic synthesis to avoid environmental contaminations and also with lower energy costs. We will use our long year experience with construction and analysis of recombinant DNA as well as with heterologous protein expression in bacteria and/or yeasts.
Duration: 1.7.2015 - 30.6.2019

Syntetická bioló - Synthetic biology for the production of new biologically active compounds in streptomycetes
Syntetická biológia pre produkciu nových biologicky aktívnych látok u streptomycét
Program: APVV
Project leader: RNDr. Kormanec Ján DrSc.
Annotation:Bacteria of the genus Streptomyces produce large amount of secondary metabolites, including the majority of antibiotics. Genes for secondary metabolites are located on physically clustered regions. Previously, we identified a gene cluster for antibiotic auricin in Streptomyces aureofaciens CCM3239, which was located on a large stable linear plasmid pSA3239. Sequence analysis of the cluster revealed huge amount of regulatory genes. Their analysis indicated a complex regulation that is responsible for an exceptional production of auricin in a narrow interval of growth. Structural analysis of auricin and the phenotypic analysis of its biosynthetic genes revealed unique mechanism of auricin biosynthesis. The main aim of the project is application of synthetic biology for production of new biologically active compounds in Streptomyces using the biosynthetic genes from the auricin cluster and other polyketide antibiotic clusters. For this purpose the genomic, transcriptomic, and phenotypic analysis of the strain S. aureofaciens CCM3239 will be done, which will reveal new gene clusters for biologically active compounds, as well as strong regulated promoters for their application in synthetic biology. Likewise, new regulatory circuits will be investigated using regulatory genes and promoters from the auricin cluster, as well as strong promoters identified by transcriptomic analysis for their application in synthetic biology. In addition, new vectors on the basis of the large linear plasmid pSA3239 will be prepared to introduce large gene clusters for biologically active compounds into Streptomyses strains for their high and stable production. The results will contribute to the knowledge about regulation of secondary metabolites and may reveal new biologically active compounds with efficient therapeutic properties. Likewise, our results may be helpful in the stable and increased production of clinically important biologically active compounds (antibiotics, cytostatic agents).
Duration: 1.7.2016 - 31.12.2019

Structural and functional studies on human ryanodine receptor 2 RIH domains together with ligands for the development of new drugs to treat cardiac arrhythmias.
Štruktúra a funkcia RIH domén ľudského ryanodínového receptora 2, ich interakcii s ligandami ako základ pre vývoj liečiv v terapii srdcových arytmií.
Program: VEGA
Project leader: Mgr. Bauerová Vladena PhD.
Annotation:Ryanodine receptor 2 is a calcium channel which releases Ca2+ from the sarcoplasmic reticulum (SR) into the cytoplasm of myocytes, thereby triggering regular heart contraction. Several mutations have been identified in the ryr2 gene which disturb the proper functioning of this channel, leading to life threatening heart arrhythmias. Recent investigations have suggested that dantrolene and K201/JTV519 may correct the abnormal RyR2-mediated Ca2+ release from SR associated with heart failure. The main aim of this project is to characterize in more detail the interactions between dantrolene and K201/JTV519 and their binding sites, which are located on the RyR2 RIH domains. This would provide a deeper understanding of how these drugs exert their effects at a molecular level, and lay a foundation for the design of new, more efficient drugs for the treatment of cardiac arrhythmias.
Duration: 1.1.2016 - 31.12.2019

Formation and regulation of higher-order septin structures
Tvorba a regulácia štruktúr vyššieho poriadku septínov
Program: VEGA
Project leader: RNDr. Farkašovský Marian CSc.
Annotation:Septins are conserved guanosine phosphate–binding proteins involved in cytokinesis and other essential membrane remodeling functions. They form heterooligomeric complexes, which polymerize into nonpolar filaments and are found in all eukaryotes, except plants. Septin structures at division plane serve as a scaffold for the localized assembly of various proteins and as diffusion barrier for membrane-associated proteins. These proteins generate different higher-order structures depending on particular stage of the cell cycle. The mechanisms of formation and localization of septin structures determine also their functionality. Posttranslational modifications and interacting partners are widely accepted regulators of septin filament function. Gic1/2, Bni5,Cdc42 and particular kinases are important for the initial septin ring, S/G2 “hour-glass” and double ring structure formation, respectively. We will focus in our project on the role of these proteins in assembly of septin complex to higher order structures.
Duration: 1.1.2015 - 31.12.2018

Formation of proteinaceous shell of Bacillus subtilis spores– studies of protein-protein interactions and self-assembly of coat proteins.
Tvorba proteínového obalu spór Bacillus subtilis– štúdium proteín-proteínových interakcií a samo-organizujúcich sa vlastností obalových proteínov.
Program: VEGA
Project leader: Ing. Krajčíková Daniela CSc.
Annotation:B. subtilis spore coat, the outermost proteinaceous layer of spore, has been a captivating object of research because of several reasons. Highly resilient spores of Bacillus and Clostridium species represent infection particles for a wide range of diseases, costing billions of euros for animal and human healthcare and causing significant economical losses in food industry. Moreover, spores are able to withstand the extreme ambient conditions and the spore coat is one of the most complex bacterial structures. Our research will be focused on the study of self-assembly of individual proteins. Also, we are interested how coat proteins are interacting thus forming such a tough shell. Contemplating the attractive potential of spores and spore coat proteins for practical application, we are going to explore the possibility of functionalization of spore or individual coat proteins, that could be, considering their ability to form highly ordered structures, used for a development of new bio-nanomaterials.
Duration: 1.1.2018 - 31.1.2020

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Úloha kalpainom vytváraných degrónov v regulácii transportérov neurotransmiterov
Program: VEGA
Project leader: RNDr. Jurský František CSc.
Duration: 1.1.2017 - 31.12.2020

INTERLIPID - The role of organelle interactions in lipid homeostasis
Úloha medziorganelových interakcií v lipidovej homeostáze
Program: APVV
Project leader: RNDr. Pevala Vladimír PhD.
Duration: 1.7.2016 - 30.6.2020

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Úloha PDZ interakcií v regulácii transportérov neurotransmiterov
Program: VEGA
Project leader: Mgr. Baliová Martina PhD.
Duration: 1.1.2017 - 31.12.2020

Effect of honeybee glucose oxidase on honey antibacterial properties and characterisation its production and activity in hypopharyngeal glands of honeybee (Apis mellifera)
Vplyv včelieho enzýmu glukózooxidáza na antibakteriálne vlastnosti medu a charakterizácia jeho produkcie a aktivity v podhltanových žľazách včely medonosnej (Apis mellifera)
Program: VEGA
Project leader: Ing. Majtán Juraj PhD.
Duration: 1.1.2018 - 31.12.2021

DIAWAT - Research of boron doped diamond films for highly effective removal of pharmaceuticals, drugs and resistant types of microorganisms from waters
Výskum bórom dopovaných diamantových vrstiev pre vysokoúčinné odstraňovanie liečiv, drog a rezistentných typov mikroorganizmov z vôd
Program: APVV
Project leader: Dr. Pangallo Domenico DrSc.
Duration: 1.7.2017 - 30.6.2020

SPORDIV - Assembly of protein complexes during asymmetric cell division in sporulating cells of Bacillus subtilis.
Vytváranie proteínových komplexov počas asymetrického bunkového delenia v sporulujúcich bunkách Bacillus subtilis.
Program: APVV
Project leader: RNDr. Barák Imrich DrSc.
Annotation:Bacillus subtilis is an internationally-recognized model organism, whose physiology, biochemistry and genetics has been studied for many years. This organism is used for decades to study the mechanisms of cell division and especially differentiation process, called sporulation. During this process very resistant spores are developed which can lie dormant and then germinate. This resistance of spores presents threats as well as potential benefits to human health and welfare. Botulism and tetanus are infectious diseases transmitted by spores, while spores of Clostridium difficile are responsible for hospital-acquired infections that are harmful to patients and which are expensive to treat and eradicate. Spores of Bacillus cereus cause food poisoning and present a challenge to the food industry, while spores of B. anthracis, which causes anthrax, are a concern because of their potential use as agents of bioterrorism and biowarfare. In contrast, the durability of spores has seen their development for use in nano-biotechnology and as probiotics in the food industry. Meanwhile their temperature resistance and capacity to resist dessication is leading to the development of spores as delivery systems for vaccines. Despite intensive research in the field of B. subtilis sporulation, there are still crucial molecular details missing in the complex mosaic of this process. This project proposes to study the mechanisms of early stages of sporulation in B. subtilis, especially asymmetric septum formation and engulfment. The aims of the project should be achieved by employing wide range of molecular biology, biochemical, genetic, structural biology and most advanced microscopy techniques.
Duration: 1.7.2015 - 30.6.2019

IMcheck - Assessing immune-checkpoints in B cell malignancies
Zhodnotenie imunitných kontrolných bodov u B bunkových malignít
Program: APVV
Project leader: Mgr. Kľučár Ľuboš PhD.
Annotation:Cancer immunotherapy is the idea of boosting the tumor-specific adaptive immune activities instead of directly targeting cancer cells. However, cancer cells can avoid immune surveillance by suppressing immunity through activation of specific inhibitory signaling pathways, referred to as immune checkpoints. Recently, the blockade of checkpoint molecules such as PD-1, PD-L1 and CTLA-4, with monoclonal antibodies targeting these checkpoint molecules has enabled the development of breakthrough therapies in oncology, with ongoing preclinical and clinical trial in B-cell malignancies. The overall objective of this proposal is study of B cell malignancies focusing on immune checkpoints molecules evolve either in clonal tumor or immune accessory populations of tumor microenvironment. Moreover, we will evaluate blockade effect of immune checkpoints neutralizing antibodies either alone or in combination together (or with novel immunomodulatory drugs) to increase their efficacy as potential therapy for patients with B-cell malignancy. Therefore, characterizing blockage effect of immune checkpoint inhibitors, either alone or in combination, on tumor cells within the tumor microenvironment will help to better understand lymphomagenesis providing the framework of development of new immune-based therapeutic strategies.
Duration: 1.7.2017 - 30.6.2021

WineZymesNGS - Improvement of organoleptic quality of wine by application of non- Saccharomyces co-starters optimized on the basis of microflora profiling using NGS and aroma profiling
Zvýšenie organoleptickej kvality vína aplikáciou nesacharomycétových koštartérov optimalizovanou na základe analýzy mikroflóry použitím NGS a analýzy arómy
Program: APVV
Project leader: Dr. Pangallo Domenico DrSc.
Duration: 1.7.2017 - 30.6.2020

Projects total: 37